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The map label for this gene is munIM [H]

Identifier: 209400932

GI number: 209400932

Start: 3294203

End: 3294829

Strand: Direct

Name: munIM [H]

Synonym: ECH74115_3576

Alternate gene names: 209400932

Gene position: 3294203-3294829 (Clockwise)

Preceding gene: 209399242

Following gene: 209399670

Centisome position: 59.12

GC content: 49.76

Gene sequence:

>627_bases
ATGACGAAAAAATACACTCTCATTTACGCAGATCCCCCCTGGACATTCCGAGACAAAGCAACCGATGGTCAACGCGGTGC
AAGTTTTAAATATCCGGTCATGAGTCTTCTGGATATCTGCCGCCTCCCGGTATGGGAACTGGCAGCCGATAATTGCCTGT
TGGCTATGTGGTGGGTGCCTACACAACCACTTGAAGCATTGAAGGTTGTAGAAGCGTGGGGCTTTCGTCTGGTGACGATG
AAAGGATTAACCTGGAACAAATGCGGGAAAAGACAGACCGACAAGCTGGTCATGGGTATGGGTAGCACCACTCGCGCTAA
CAGCGAAGACTGCCTTTTTGCAGTGAAAGGAAATCTGCCTGAACGCATTAACGCCGGAATAATCCAGTCATTCACTGCAC
CGCGCCTTGATCACTCCCGCAAGCCGGATATGGCGCGAGAAAAACTTGTGCAACTTCTTGGCGATGTTCCCCGGATAGAA
CTGTTCGCCCGTCACACCTCGCATGGATTTGATGTCTGGGGTAACCAATGCGGAACACCATCCATTGAGATGGTTCCGGG
TATTGTTAAATTTCTGGAGAAAACCAATGAGCGAAAAAACGACGTTGACAAAGGCATCACCAGTTGA

Upstream 100 bases:

>100_bases
ATATCTATGGTCATAGCTTCGGTGCTCCACATGACGCAGCCGATTTCAGACGATGCGTTGCACTTGTTGCACTTTACATT
GATGAGTTTGAAAACGCGCT

Downstream 100 bases:

>100_bases
ATTAAGGCAGTGCCTGGAAATCGCAAATCAACTTGCCAGAAGTGGAATACGCTTTGTTCCGATCCCGATCACAGCAGATA
CAGAACTTCATCTGTTTGGT

Product: adenine methylase

Products: NA

Alternate protein names: Adenine-specific methyltransferase MunI; M.MunI [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPVWELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTM
KGLTWNKCGKRQTDKLVMGMGSTTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS

Sequences:

>Translated_208_residues
MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPVWELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTM
KGLTWNKCGKRQTDKLVMGMGSTTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS
>Mature_207_residues
TKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPVWELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMK
GLTWNKCGKRQTDKLVMGMGSTTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIEL
FARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS

Specific function: This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease [H]

COG id: COG4725

COG function: function code TK; Transcriptional activator, adenine-specific DNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MT-A70-like family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR007757 [H]

Pfam domain/function: PF05063 MT-A70 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 23502; Mature: 23370

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00092 N6_MTASE ; PS51143 MT_A70

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPVWELAADNCLLAMWWVP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCEEEEEEECC
TQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGSTTRANSEDCLFAVKGNLP
CCHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCC
ERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIELFARHTSHGFDVWGNQCGTP
HHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCC
SIEMVPGIVKFLEKTNERKNDVDKGITS
CHHHHHHHHHHHHHHCHHHHHHHCCCCC
>Mature Secondary Structure 
TKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPVWELAADNCLLAMWWVP
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCEEEEEEECC
TQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGSTTRANSEDCLFAVKGNLP
CCHHHHHHHHHHCCEEEEEECCCCHHHCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCC
ERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIELFARHTSHGFDVWGNQCGTP
HHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCC
SIEMVPGIVKFLEKTNERKNDVDKGITS
CHHHHHHHHHHHHHHCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181741 [H]