Click here to switch to the map view.

The map label for this gene is adrB [H]

Identifier: 209400930

GI number: 209400930

Start: 2406550

End: 2408148

Strand: Direct

Name: adrB [H]

Synonym: ECH74115_2544

Alternate gene names: 209400930

Gene position: 2406550-2408148 (Clockwise)

Preceding gene: 209400418

Following gene: 209399568

Centisome position: 43.19

GC content: 50.22

Gene sequence:

>1599_bases
ATGCAAAAAGCACAACGGATCATTAAAACCTATCGCCGTAATCGAATGATTGTTTGTACGATTTGCGCACTCGTTACGCT
CGCTTCGACCCTGAGCGTGCGATTTATTTCACAGCGTAACTTAAATCAACAACGGGTAGTACAATTCGCCAATCACGCTG
TAGAGGAATTAGATAAAGTACTGCTTCCCCTACAGGCAGGTAGCGAAGTCTTGCTTCCGCTGATTGGTCTGCCCTGCTCT
GTCGCCCATTTGCCATTACGTAAACAGGCGGCAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACT
TTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTT
TACGCCTGACGACCGACCGTGTCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGAGCAGTAGCAAT
GCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAGCGC
CAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCACAACCTGAAGACAATGAAA
ACTATCAGGTTTCTTCGCAACGCTTTTCTTTTACCATTAACGTTAATGGTCCGGGGGCTCCGGCGCTGGCATGGCACTAT
CTTCCAACACAATTACCGCTGGCGGTACTGCTAAGTTTACTGGTGGGCTACATCGCCTGGCTGGCGACCGCTTACCGAAT
GAGCTTTTCCCGCGAAATCAATCTGGGCCTGGCGCAACATGAGTTCGAATTGTTCTGTCAGCCTTTGCTTAATGCGCGCA
GCCAGCAATGTATTGGTGTAGAGATTTTGCTGCGCTGGAACAATCCGCGTCAGGGCTGGATTTCACCGGATGTGTTTATT
CCTATCGCGGAAGAACATCATTTAATTGTGCCACTGACCCGCTATGTGATGGCAGAAACCATTCGTCAGCGCCATGTTTT
CCCGATGAGTAGTCAGTTTCATGTTGGCATTAACGTCGCACCCAGCCATTTTCGCCGTGGTGTGCTGATAAAAGATCTCA
ATCAGTACTGGTTTAGCGCTCACCCGATTCAGCAACTGATCCTCGAAATCACCGAACGCGATGCCTTACTGGATGTTGAT
TATCGGATTGCCCGCGAGCTACATCGTAAAAACGTCAAACTGGCGATTGATGACTTCGGCACCGGCAACAGTTCATTTTC
CTGGCTTGAAACATTACGTCCTGACGTGCTGAAAATTGATAAGTCATTTACCGCAGCTATAGGTTCTGACGCGGTTAACT
CGACGGTGACCGATATCATCATCGCGCTGGGGCAAAGACTGAATATTGAACTGGTGGCGGAGGGCGTGGAAACGCAAGAA
CAGGCGAAGTATTTGCGCCGTCATGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTT
TCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAA

Upstream 100 bases:

>100_bases
GGCGAATTTATCCCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCAT
ACGCTTACAGAGTGGCCCGC

Downstream 100 bases:

>100_bases
CGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGA
TGGTGATATGCAGTGGCCCT

Product: cyclic diguanylate phosphodiesterase (EAL) domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQRVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCS
VAHLPLRKQAAKLQTVRSIGLVQDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVSSQRFSFTINVNGPGAPALAWHY
LPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFI
PIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVD
YRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAEGVETQE
QAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR

Sequences:

>Translated_532_residues
MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQRVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCS
VAHLPLRKQAAKLQTVRSIGLVQDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVSSQRFSFTINVNGPGAPALAWHY
LPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFI
PIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVD
YRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAEGVETQE
QAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR
>Mature_532_residues
MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQRVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCS
VAHLPLRKQAAKLQTVRSIGLVQDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVSSQRFSFTINVNGPGAPALAWHY
LPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFI
PIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVD
YRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAEGVETQE
QAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR

Specific function: May serve as a negative regulator of cellulose synthesis (as has been suggested for S.typhimurium); over-expression inhibits cell aggregation in strains able to produce adhesive curli fimbriae. Cyclic-di-GMP is a second messenger which controls cell surfa

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI87081980, Length=532, Percent_Identity=99.0601503759399, Blast_Score=1078, Evalue=0.0,
Organism=Escherichia coli, GI1788502, Length=515, Percent_Identity=28.3495145631068, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87081845, Length=532, Percent_Identity=27.4436090225564, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786507, Length=247, Percent_Identity=39.2712550607287, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790496, Length=259, Percent_Identity=35.1351351351351, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87081921, Length=261, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081743, Length=277, Percent_Identity=32.4909747292419, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI226510982, Length=262, Percent_Identity=31.6793893129771, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787541, Length=259, Percent_Identity=30.5019305019305, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788849, Length=239, Percent_Identity=31.3807531380753, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87082096, Length=242, Percent_Identity=29.7520661157025, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787055, Length=254, Percent_Identity=30.3149606299213, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 59744; Mature: 59744

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQRVVQFANHAVEELDKV
CCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHH
LLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLVQDGTLYCSSIFGYRNVPV
HHCCCCCCHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCH
VDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSNAGVMEMINIDLLTAMLLEPQ
HHHHHHCCCCCHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
LPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVSSQRFSFTINVNGPGAPALAWHY
CCCCCCCEEEEECCEEEEECCHHHCCCCCCCCCCEEEECEEEEEEEEECCCCCCCEEEEC
LPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHCCCCCCCEEE
EILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGINVA
EEEEEECCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
PSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVKLAIDDFG
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCEEEEECCC
TGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAEGVETQE
CCCCCHHHHHHCCCHHEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
QAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQRVVQFANHAVEELDKV
CCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHEEHHHCCCCHHHHHHHHHHHHHHHHHH
LLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLVQDGTLYCSSIFGYRNVPV
HHCCCCCCHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCH
VDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSNAGVMEMINIDLLTAMLLEPQ
HHHHHHCCCCCHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
LPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVSSQRFSFTINVNGPGAPALAWHY
CCCCCCCEEEEECCEEEEECCHHHCCCCCCCCCCEEEECEEEEEEEEECCCCCCCEEEEC
LPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQCIGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHCCCCCCCEEE
EILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHVGINVA
EEEEEECCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
PSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVKLAIDDFG
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCEEEEECCC
TGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIELVAEGVETQE
CCCCCHHHHHHCCCHHEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
QAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIMPLR
HHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]