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The map label for this gene is nrdF [H]

Identifier: 209400851

GI number: 209400851

Start: 3631888

End: 3632847

Strand: Direct

Name: nrdF [H]

Synonym: ECH74115_3920

Alternate gene names: 209400851

Gene position: 3631888-3632847 (Clockwise)

Preceding gene: 209398305

Following gene: 209398437

Centisome position: 65.18

GC content: 48.54

Gene sequence:

>960_bases
ATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAATCGCCTGAC
CAGCAATTTCTGGCTACCAGAAAAGGTGCCGCTGTCGAACGATATTCCTGCCTGGCAGACATTAACTGTCGTAGAACAAC
AACTGACGATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCGCTGATGCCC
GATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTC
GATTTTCTCGACGCTGTGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCGCCGTTGCAGCGAA
AAGCACAGATTATTCAGCAACATTACCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTT
TTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCT
GATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGAC
AACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTG
TACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTA
CGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATC
ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAATATGAAGACTGGAATTTCTGA

Upstream 100 bases:

>100_bases
CAAAGGTATCAAAACGCTCTATTACATCCGGCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCT
GTGCACTTTAAGGAATATCT

Downstream 100 bases:

>100_bases
GGGTGTTATTTTCAAAAATATCACTACCCGCGACAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATATTTT
GTGGTGATCTACACTGATAC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF

Sequences:

>Translated_319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF
>Mature_319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=319, Percent_Identity=99.6865203761756, Blast_Score=658, Evalue=0.0,
Organism=Escherichia coli, GI1788567, Length=265, Percent_Identity=24.9056603773585, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36492; Mature: 36492

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
HHHHHHHHHHHCCCCEEEEEEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HHCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
GSSYVMGKAVETEYEDWNF
CCCEEECCHHCCCCCCCCC
>Mature Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYTDEL
HHHHHHHHHHHCCCCEEEEEEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HHCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
GSSYVMGKAVETEYEDWNF
CCCEEECCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2649479; 8195103 [H]