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The map label for this gene is sppA [H]

Identifier: 209400845

GI number: 209400845

Start: 2354643

End: 2356499

Strand: Direct

Name: sppA [H]

Synonym: ECH74115_2486

Alternate gene names: 209400845

Gene position: 2354643-2356499 (Clockwise)

Preceding gene: 209399515

Following gene: 209400024

Centisome position: 42.26

GC content: 52.13

Gene sequence:

>1857_bases
ATGCGAACCCTTTGGCGATTTATTGCCGGATTTTTTAAATGGACGTGGCGTCTGCTGAATTTCGTCCGTGAAATGGTACT
TAACCTGTTCTTTATTTTTCTCGTGCTGGTTGGTGTGGGGATCTGGATGCAGGTCAGTGGTGGTGATTCGAAAGAAACGG
CCAGTCGTGGTGCACTGCTGCTGGACATTTCTGGTGTGATCGTCGATAAACCCGACAGTTCTCAGCGGTTTAGTAAATTA
AGCCGCCAACTACTTGGTGCCAGTTCCGATCGTCTGCAGGAAAACTCACTGTTTGATATCGTCAATACCATTCGCCAGGC
GAAGGATGACCGCAATATCACCGGTATCGTGATGGATCTGAAAAACTTCGCAGGCGGCGACCAACCGTCTATGCAGTACA
TCGGCAAAGCCCTGAAAGAGTTTCGTGACAGCGGGAAACCGGTTTATGCCGTTGGCGAGAACTACAGCCAGGGGCAATAT
TATCTCGCCAGTTTCGCCAATAAAATTTGGCTGTCACCGCAAGGCGTGGTGGATTTGCACGGTTTTGCTACCAACGGTCT
GTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAAC
CGTTTATTCGTGATGACATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTG
AATACTGTTGCCGCTAACCGGCAGATCCCTGCTCAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTTTAACCAA
AACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAG
CACTGACCAAAGAGTTCGGCTGGAGTAAGGCTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACG
CCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTCGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGT
TGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATA
GCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTT
GTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCAC
CCTGACCGGTTCTATTGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGTTCATACTGATG
GTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCAGATGATGCAGTTAAGC
ATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGCTGATGCGCGTCATTCGACGCCGGAGCAGATTGATAAAATCGC
CCAGGGCCACGTCTGGACCGGTCAGGACGCAAAAGCTAACGGGCTGGTCGATAGTCTCGGGGATTTCGATGATGCGGTCG
CCAAAGCAGCAGAGCTGGCAAAAGCGAAACAGTGGCATCTGGAATACTACGTTGATGAACCGACCTTCTTCGACAAAGTG
ATGGACAACATGTCTGGTTCTGTCCGGGCAATGTTGCCAGATGCGTTCCAGGCCATGTTACCTGCACCGCTGGCCTCGGT
AGCCTCTACCGTTAAAAGTGAAAGCGACAAGCTGGCGGCGTTTAACGACCCACAAAACCGTTATGCGTTTTGCCTGACCT
GCGCCAACGTGCGTTAA

Upstream 100 bases:

>100_bases
AGCACAGTCCGCAATTCCTGCTGACAAGTGCCGGTTGGGTCATTACGATAACCACATCTATTGCGCCTGTGACAGGTGTG
ACCTTAAGTTGGGAGAATAC

Downstream 100 bases:

>100_bases
ATCCTGCAAAAATGTGGCCGATATGCTGTCGGCCACACTCCTGAATCGGACTGTCTTTCACTCCCTCTCTCCACTACATT
CCCTTGCTCATTTAGCATCT

Product: protease 4

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKL
SRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
NTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKT
PADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVV
VSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQMMQLS
IENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKV
MDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR

Sequences:

>Translated_618_residues
MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKL
SRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
NTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKT
PADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVV
VSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQMMQLS
IENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKV
MDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR
>Mature_618_residues
MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKL
SRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
NTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKT
PADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVV
VSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQMMQLS
IENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKV
MDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=618, Percent_Identity=99.1909385113269, Blast_Score=1261, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=175, Percent_Identity=29.1428571428571, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67144; Mature: 67144

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCEEE
LDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDL
EEECCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
KNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGVVDLH
HHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEC
GFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
CEECCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
NTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFG
HHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WSKADKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDAR
CCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHCC
LDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANY
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEECCCCE
IVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQMMQLS
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHH
IENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELA
HHCCHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHHHHHH
KAKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAA
HHHHEEEEEEECCCHHHHHHHHCCCCCEEECCHHHHHHHCCCHHHHHHHHHHCCCCCEEE
FNDPQNRYAFCLTCANVR
CCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCEEE
LDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDL
EEECCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
KNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGVVDLH
HHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEC
GFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
CEECCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
NTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFG
HHHHCCCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WSKADKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDAR
CCCCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHCC
LDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANY
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEECCCCE
IVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQQMMQLS
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHH
IENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELA
HHCCHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCCCHHHHCCHHHHHHHHHHHH
KAKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAA
HHHHEEEEEEECCCHHHHHHHHCCCCCEEECCHHHHHHHCCCHHHHHHHHHHCCCCCEEE
FNDPQNRYAFCLTCANVR
CCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]