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The map label for this gene is garP

Identifier: 209400778

GI number: 209400778

Start: 4115891

End: 4117225

Strand: Reverse

Name: garP

Synonym: ECH74115_4439

Alternate gene names: 209400778

Gene position: 4117225-4115891 (Counterclockwise)

Preceding gene: 209398713

Following gene: 209397366

Centisome position: 73.89

GC content: 50.64

Gene sequence:

>1335_bases
ATGATTCTGGACACCGTTGACGAAAAAAAGAAAGGCGTGCATACCCGCTATTTAATATTACTGATTATTTTTATTGTTAC
CGCCGTTAACTACGCCGATCGTGCAACGCTGTCTATTGCTGGTACCGAAGTGGCAAAAGAGTTGCAGTTAAGTGCGGTTT
CGATGGGTTACATCTTTTCTGCTTTTGGCTGGGCCTACTTGCTGATGCAAATCCCCGGCGGCTGGCTGCTTGATAAGTTT
GGCTCGAAAAAAGTTTACACCTACAGCCTCTTTTTCTGGTCGCTATTCACCTTCCTGCAAGGCTTTGTTGATATGTTCCC
GCTGGCCTGGGCAGGGATCTCCATGTTCTTTATGCGCTTTATGCTCGGCTTCTCGGAAGCGCCATCATTCCCGGCGAACG
CCCGTATTGTCGCCGCCTGGTTCCCGACGAAAGAACGTGGTACTGCCTCCGCCATCTTTAACTCGGCGCAATATTTCTCG
CTGGCGCTCTTTTCGCCTCTGCTTGGCTGGCTGACTTTCGCCTGGGGCTGGGAGCACGTCTTTACCGTTATGGGGGTGAT
TGGTTTTGTGCTGACGGCGCTGTGGATCAAATTGATTCATAACCCGACAGATCACCCACGTATGTCTGCGGAAGAGCTGA
AGTTTATCTCTGAAAATGGCGCGGTGGTCGATATGGACCACAAAAAGCCGGGCAGTGCGGCAGCAAGCGGACCCAAACTG
CATTACATCAAGCAATTGCTCTCTAACCGCATGATGTTGGGCGTATTTTTCGGACAATATTTTATCAACACCATCACCTG
GTTCTTCCTCACCTGGTTCCCGATTTATCTGGTGCAGGAAAAAGGTATGTCGATTCTGAAAGTGGGTCTGGTCGCCTCGA
TTCCAGCACTGTGTGGTTTTGCGGGCGGCGTGCTGGGAGGTGTCTTCTCGGATTATCTGATCAAACGCGGTTTATCCCTG
ACCCTGGCACGTAAGCTACCGATTGTGCTGGGAATGTTGCTGGCTTCCACCATCATCTTATGTAACTACACCAACAACAC
CACGCTGGTGGTCATGCTGATGGCGCTGGCTTTCTTTGGTAAAGGATTTGGTGCGCTGGGCTGGCCGGTGATTTCTGACA
CCGCGCCTAAAGAGATTGTTGGCCTCTGCGGCGGCGTCTTTAACGTCTTTGGCAATGTTGCCTCCATTGTCACTCCACTG
GTGATTGGCTACCTGGTAAGTGAACTGCACTCCTTCAATGCAGCACTGGTTTTCGTGGGATGTTCAGCGCTAATGGCGAT
GGTCTGCTACCTCTTCGTAGTTGGCGACATTAAACGTATGGAATTGCAGAAATAA

Upstream 100 bases:

>100_bases
CTAATTCCCGAGTTCATTGTCGTTACGGCATAAATGAAACTCATAAATAACATCTTCCCCATAATAATAAAAATCAGCAT
AAGTACCCGAGGTAAATAAA

Downstream 100 bases:

>100_bases
GCAAAGGTATAAGCGATGAATAACGATGTTTTCCCGAATAAATTCAAAGCCGCACTGGCTGCGAAACAGGTACAAATTGG
TTGCTGGTCAGCACTCTCTA

Product: galactarate permease GarP

Products: NA

Alternate protein names: D-galactarate permease

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKF
GSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSL
TLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPL
VIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK

Sequences:

>Translated_444_residues
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKF
GSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSL
TLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPL
VIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK
>Mature_444_residues
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKF
GSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSL
TLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPL
VIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK

Specific function: Uptake of D-galactarate

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Phthalate permease family

Homologues:

Organism=Homo sapiens, GI9945322, Length=425, Percent_Identity=24.7058823529412, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI21322234, Length=420, Percent_Identity=24.5238095238095, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI148743791, Length=375, Percent_Identity=26.1333333333333, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI9966811, Length=433, Percent_Identity=23.094688221709, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4885441, Length=377, Percent_Identity=27.8514588859416, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI6912666, Length=425, Percent_Identity=24, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI5031955, Length=337, Percent_Identity=27.5964391691395, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI148728191, Length=313, Percent_Identity=25.5591054313099, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI154350196, Length=417, Percent_Identity=23.9808153477218, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI223718438, Length=222, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789515, Length=444, Percent_Identity=100, Blast_Score=897, Evalue=0.0,
Organism=Escherichia coli, GI1789152, Length=434, Percent_Identity=57.6036866359447, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI87082320, Length=435, Percent_Identity=40.9195402298851, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI2367379, Length=434, Percent_Identity=30.184331797235, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI2367193, Length=410, Percent_Identity=25.609756097561, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1788573, Length=438, Percent_Identity=23.5159817351598, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI115533556, Length=437, Percent_Identity=26.5446224256293, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI115533554, Length=434, Percent_Identity=26.4976958525346, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17552542, Length=377, Percent_Identity=24.9336870026525, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535049, Length=419, Percent_Identity=23.6276849642005, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17560030, Length=403, Percent_Identity=22.8287841191067, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71997452, Length=351, Percent_Identity=25.0712250712251, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17570335, Length=247, Percent_Identity=26.7206477732794, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141318, Length=404, Percent_Identity=20.2970297029703, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25141316, Length=404, Percent_Identity=20.2970297029703, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193205400, Length=283, Percent_Identity=24.3816254416961, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71991448, Length=210, Percent_Identity=30, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71991453, Length=210, Percent_Identity=30, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32565916, Length=289, Percent_Identity=22.4913494809689, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25141341, Length=375, Percent_Identity=20.2666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25151055, Length=247, Percent_Identity=25.9109311740891, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32565914, Length=274, Percent_Identity=21.1678832116788, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17569727, Length=338, Percent_Identity=23.6686390532544, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI86561091, Length=286, Percent_Identity=22.3776223776224, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554148, Length=405, Percent_Identity=22.962962962963, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193207426, Length=389, Percent_Identity=23.1362467866324, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648416, Length=454, Percent_Identity=24.6696035242291, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356189, Length=454, Percent_Identity=24.6696035242291, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24654215, Length=468, Percent_Identity=23.5042735042735, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922400, Length=460, Percent_Identity=23.9130434782609, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20130135, Length=426, Percent_Identity=25.5868544600939, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655271, Length=423, Percent_Identity=25.2955082742317, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24639764, Length=422, Percent_Identity=23.696682464455, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45550460, Length=414, Percent_Identity=24.8792270531401, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583789, Length=413, Percent_Identity=25.90799031477, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28573708, Length=475, Percent_Identity=24.6315789473684, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28573707, Length=475, Percent_Identity=24.6315789473684, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641682, Length=405, Percent_Identity=22.4691358024691, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24655255, Length=376, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24584153, Length=430, Percent_Identity=24.4186046511628, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653727, Length=372, Percent_Identity=25.8064516129032, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI62484226, Length=389, Percent_Identity=25.706940874036, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI20130167, Length=469, Percent_Identity=24.0938166311301, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129235, Length=395, Percent_Identity=26.0759493670886, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24580790, Length=381, Percent_Identity=24.1469816272966, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550364, Length=453, Percent_Identity=25.1655629139073, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24658658, Length=449, Percent_Identity=24.0534521158129, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28573299, Length=434, Percent_Identity=25.1152073732719, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920686, Length=327, Percent_Identity=22.6299694189602, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GARP_ECO57 (P0AA81)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85974
- PIR:   E91129
- RefSeq:   NP_289698.1
- RefSeq:   NP_312032.1
- ProteinModelPortal:   P0AA81
- EnsemblBacteria:   EBESCT00000028019
- EnsemblBacteria:   EBESCT00000058911
- GeneID:   916162
- GeneID:   958584
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4479
- KEGG:   ecs:ECs4005
- GeneTree:   EBGT00050000008818
- HOGENOM:   HBG338043
- OMA:   STSEDKC
- ProtClustDB:   CLSK880597
- BioCyc:   ECOL83334:ECS4005-MONOMER
- InterPro:   IPR004744
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00893

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49010; Mature: 49010

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11269410)-; HASH(0xc0de6f8)-; HASH(0xff2a9a0)-; HASH(0x1064f228)-; HASH(0xecbb5d4)-; HASH(0xb726834)-; HASH(0xefe9d4c)-; HASH(0x114d45c0)-; HASH(0x113324d4)-; HASH(0x10eebdd8)-; HASH(0x118176c8)-; HASH(0x1159a994)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFS
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
AFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRF
HHHHHHHHHHCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHV
HHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCHH
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHC
KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
CSALMAMVCYLFVVGDIKRMELQK
HHHHHHHHHHHHHHCCHHHHHCCH
>Mature Secondary Structure
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFS
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
AFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRF
HHHHHHHHHHCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHV
HHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCHH
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHC
KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
CSALMAMVCYLFVVGDIKRMELQK
HHHHHHHHHHHHHHCCHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796