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The map label for this gene is yqiG [H]

Identifier: 209400765

GI number: 209400765

Start: 352453

End: 353595

Strand: Reverse

Name: yqiG [H]

Synonym: ECH74115_0345

Alternate gene names: 209400765

Gene position: 353595-352453 (Counterclockwise)

Preceding gene: 209399836

Following gene: 209398292

Centisome position: 6.35

GC content: 44.09

Gene sequence:

>1143_bases
ATGACAAATAAACACCCATCATTGTTTTCTCCTTTTATGCTGACAGAAAAAATAAAGCTACGTAATCGTATCGTTATGGC
TCCTATGACGACCTGGTCAGCGAATCCTGATGGAACCATCTCTGAGCAAGAACTCGAATTTTATAAACGTCGCTCACAAA
ATGTTGGTTTAGTAATAACAGGATGTACCTATGTCACGCCAAGCGGCATTGGGTTTACTCATGAATTTGCCGCGTATGAC
GACCGTTTCATTAACAGCCTTGAAAAGTTAGCAGCGGCAGCCAAAAGTGGCGGTGCTCCGGCTATTTTGCAGATTTTCCA
CGCCGGTAATAAAGCCATCCCGGAGCTTGTACCAAATAACGATGTTATTAGTGCCAGCGCATCAAGCGTAAAATCTGGTG
ACTTTATGAAGAGAGTGGTACAAAGCAGGGAAATGACTGAAAACGAAATTCAGGAAACCATTAGAGCATTCGGAGATGTC
ACAAAAAGGGCGATTAAAGCAGGTTTTGATGGCATTGAACTTCATGGTGCGCACGGATTCCTTCTACAGAATTTTTTCTC
CCCTCTATTTAACCAGCGTAATGACCGATGGGGAGGAGATCTCGAAGGACGTATGCGCTTCCCGCTGGCCGTATTACAGG
AAGTAAAAAATGTAGTCTATGAATATGCGACAAAGCCCTTTGCCATCGGTTACAGAATATCTCCTGAAGAATCTGCAACA
GGGGGGCTGCGGATAGAGGATACTTATAAACTCCTTGATCGGCTCATTTCATCCGGTATTTCGTATATACATACCTCGCT
GGTCAGTATTAACGATAGCTACCCTGTTGAGTCGCCAAATGGCCCCCGAACTATTGAATTGATTTTAAATCATATTGCAG
GCAGAGTGCCGGTAATCGCTGCAGGTAAAATACGCACACCGTCTCAGGCTCAAGAAGCAATTTCCACTGGCCTGCCACTT
GTTGCGATCGGTAAAGGGCTGGTGATAAATCCAGAATGGGTGACTTTAGCTGAATCAGGTAGAGGCCATGAAATTCAAAC
AACCCTTAATCCACAACTTGTTCCTGAATTAACCATTCCGGATAAACTATGGGATCAAATTCAGGCATCGAAAGGAACTG
GCTGGTTTCCGTTAATGGATTAA

Upstream 100 bases:

>100_bases
GGGATTATAAATAAAGCCAGAACTCTTGGTTCCAAAGCTGGCAACAACTAACCAGTTAAAACATACGCATCAAAATCAGA
TAAACATTTGAGGTATTGTT

Downstream 100 bases:

>100_bases
ATATCTATTACTCTCCCGAGAGTAAAACTATCAAATCAATGAGTAACCGATACAGCCTCACAAATTCTTTCAGATAATTA
ACCCGTCACTGCGGCACGTT

Product: oxidoreductase, FAD/FMN-binding

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYD
DRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPL
VAIGKGLVINPEWVTLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD

Sequences:

>Translated_380_residues
MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYD
DRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPL
VAIGKGLVINPEWVTLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD
>Mature_379_residues
TNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDD
RFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVT
KRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESATG
GLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLV
AIGKGLVINPEWVTLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=352, Percent_Identity=28.4090909090909, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=28.3333333333333, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=278, Percent_Identity=28.0575539568345, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=375, Percent_Identity=26.1333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=260, Percent_Identity=28.4615384615385, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=277, Percent_Identity=27.0758122743682, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=272, Percent_Identity=27.2058823529412, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=221, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=185, Percent_Identity=29.7297297297297, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=355, Percent_Identity=25.6338028169014, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325086, Length=358, Percent_Identity=24.5810055865922, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 41825; Mature: 41694

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVIT
CCCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNN
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
DVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGIELHGAHGF
CEEECCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
LLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
HHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
AGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWVTLAESGRGHEIQTTLNPQLVPELTIP
ECCCCCCHHHHHHHHCCCCEEEECCCEEECCCEEEEECCCCCCEEEEECCCCCCCCCCCC
DKLWDQIQASKGTGWFPLMD
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQELEFYKRRSQNVGLVIT
CCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNN
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
DVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDVTKRAIKAGFDGIELHGAHGF
CEEECCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHH
LLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNVVYEYATKPFAIGYRISPEESAT
HHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
GGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPVESPNGPRTIELILNHIAGRVPVIA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
AGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWVTLAESGRGHEIQTTLNPQLVPELTIP
ECCCCCCHHHHHHHHCCCCEEEECCCEEECCCEEEEECCCCCCEEEEECCCCCCCCCCCC
DKLWDQIQASKGTGWFPLMD
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]