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The map label for this gene is nudG [H]

Identifier: 209400758

GI number: 209400758

Start: 2347296

End: 2347703

Strand: Direct

Name: nudG [H]

Synonym: ECH74115_2479

Alternate gene names: 209400758

Gene position: 2347296-2347703 (Clockwise)

Preceding gene: 209397120

Following gene: 209399515

Centisome position: 42.13

GC content: 54.17

Gene sequence:

>408_bases
ATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGA
TCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCGGATGAAAGCCAGCAGCAGGCGCTGGTGCGTGAGTTAA
ACGAAGAATTGGGCATCGAAGCAACTGTGGGTGAGTATGTTGCCAGCCATCAGCGAGAAGTTTCGGGCCGGATTATCCAT
CTTCATGCCTGGCACGTACCCGACTTCCACGGGACGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGA
AGAGGCGCTGCAATATCCGCTGGCTCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAG
CGGATTAG

Upstream 100 bases:

>100_bases
TGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCT
TATCTGCCCGTGTGTAATTT

Downstream 100 bases:

>100_bases
TGCTAAGGGTTTTGTCATCCCGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTTA
TCCTGATAAACGCAGCACTG

Product: pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDESQQQALVRELNEELGIEATVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMALRAARPAD

Sequences:

>Translated_135_residues
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDESQQQALVRELNEELGIEATVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMALRAARPAD
>Mature_135_residues
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDESQQQALVRELNEELGIEATVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMALRAARPAD

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=135, Percent_Identity=98.5185185185185, Blast_Score=268, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 14976; Mature: 14976

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDESQQQALVRELNEELGIE
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
ATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIP
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCH
LLEAFMALRAARPAD
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDESQQQALVRELNEELGIE
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
ATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIP
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCH
LLEAFMALRAARPAD
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]