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The map label for this gene is yhjK [H]

Identifier: 209400445

GI number: 209400445

Start: 4528845

End: 4530833

Strand: Reverse

Name: yhjK [H]

Synonym: ECH74115_4894

Alternate gene names: 209400445

Gene position: 4530833-4528845 (Counterclockwise)

Preceding gene: 209396215

Following gene: 209398499

Centisome position: 81.31

GC content: 51.99

Gene sequence:

>1989_bases
TTGCGCGTAAGTCGCTCGTTAACAATCAAGCAGATGGCAATGGTGGCAGCCGTTGTCCTGGTGTTCGTTTTTATTTTTTG
CACCGTTTTGCTGTTCCATCTGGTCCAGCAGAATCGCTATAACACGGCTACGCAACTGGAAAGCATTGCTCGCTCTGTCC
GCGAACCTTTATCTTCAGCCATTTTGAAAGGTGATATTCCCGAAGCGGAAGCTATTCTTGCCAGCATTAAACCGGCAGGC
GTGGTCAGCCGTGCCGATGTAGTGCTGCCTAACCAGTTCCAGGCGCTGCGTAAAAGTTTTATTCCAGAGCGTCCGGTGCC
GGTAATGGTTACTCGCCTGTTTGAGCTACCGGTTCAAATCTCGCTGGGCGTCTACTCGCTCGAACGTCCGGCAAATCCGC
AGCCAATTGCCTATCTGGTGCTACAGGCGGATTCCTTCCGTATGTATAAGTTCGTGATGAGCACCCTCTCAACGTTAGTG
ACCATTTACTTACTTTTGTCGTTAATATTGACGGTGGCGATTAGCTGGTGCATTAACCGCCTGATTTTGCATCCGTTACG
CAATATTGCTCGCGAACTTAACGCCATCCCAGCCCAGGAGCTTGTTGGTCACCAACTGGCATTACCGCGTCTGCATCAGG
ACGATGAAATCGGTATGTTGGTGCGCAGTTACAACCTCAACCAGCAATTGCTGCAGCGCCATTATGAAGAACAGAACGAA
AATGCGATGCGCTTCCCGGTGTCGGATTTGCCGAACAAAGCCTTGCTGATGGAGATGCTGGAGCAGGTTGTCGCGCGTAA
ACAAACCACCGCGCTGATGATCATCACCTGTGAAACTCTGCGTGATACTGCGGGCGTGCTGAAAGAGGCGCAACGAGAAA
TTCTGCTGCTGACGCTGGTGGAAAAACTCAAATCGGTACTGTCGCCACGTATGATCCTCGCGCAGATTAGCGGTTATGAC
TTTGCTGTCATTGCCAACGGTGTACATGAACCGTGGCACGCAATCACTTTGGGTCAGCAAGTGCTCACTATCATGAGCGA
GCGCCTGCCGATTGAACGTATTCAACTCCGTCCGCACTGTAGCATTAGCGTGGCGATGTTCTACGGCGATCTCACCGCCG
AACAGCTTTACAGTCGCGCTATTTCTGCGGCATTTACCGCTCGCCATAAAGGCAAGAATCAGATTCAGTTCTTTGATCCG
CAGCAGATGGAAGCCGCCCAGAAGCGGTTGACGGAAGAGAGCGATATCCTTAATGCACTGGAAAATCATCAGTTTGCTAT
TTGGTTACAGCCACAGGTCGAGATGACCAGCGGTAAACTGGTCAGTGCGGAAGTGTTACTGCGTATCCAGCAACCGGATG
GCAGTTGGGACCTGCCGGATGGCTTAATCGATCGCATTGAGTGCTGTGGGCTGATGGTTACCGTCGGTCACTGGGTGCTG
GAAGAGTCCTGTCGATTGCTTGCAGCCTGGCAAGAGCGCGGCATTATGCTGCCCTTGTCGGTAAACCTCTCTGTGCTGCA
ACTGATGCACCCGAATATGGTGGCGGATATGCTGGAACTGTTAACCCGCTATCGCATTCAGCCGGGAACACTGATTCTGG
AAGTGACAGAAAGCCGACGTATTGACGACCCTCATGCTGCGGTGGCAATCCTCCGTCCGCTGCGTAATGCCGGAGTTCGG
GTGGCGCTGGATGATTTCGGCATGGGCTACGCAGGGCTGCGTCAGCTGCAGCATATGAAATCGTTGCCAATCGACGTTCT
GAAAATCGACAAAATGTTTGTTGAAGGCTTGCCGGAAGATAGCAGCATGATTGCTGCAATTATCATGCTGGCGCAGAGCC
TGAACTTACAAATGATTGCCGAAGGCGTGGAGACTGAAGCACAACGCGACTGGCTGGCAAAAGCGGGCGTTGGTATTGCC
CAGGGCTTCCTTTTTGCTCGTCCACTCCCTATTGAAATCTTCGAAGAGAGTTACCTGGAAGAAAAGTAG

Upstream 100 bases:

>100_bases
ACCTTACGCCGACTGGTAAGTTTTCAGATAGCGCCTCTCTTAATGCCGCTGCGATCGGGTATACTCGGGCGGCAATCTGG
GATTTCCGGGGGGAGACAAT

Downstream 100 bases:

>100_bases
CTACCCCAAACTGATTACAAAACTTTAAAAAGTGCTGGTTTGTGCGAGCCAGCTCAAACTTTTTAACCTTTTTGTTTCAA
TTATGATCCAGGTACATTTC

Product: putative phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSAILKGDIPEAEAILASIKPAG
VVSRADVVLPNQFQALRKSFIPERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIGMLVRSYNLNQQLLQRHYEEQNE
NAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIITCETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYD
FAVIANGVHEPWHAITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQFFDP
QQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDGSWDLPDGLIDRIECCGLMVTVGHWVL
EESCRLLAAWQERGIMLPLSVNLSVLQLMHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVR
VALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIA
QGFLFARPLPIEIFEESYLEEK

Sequences:

>Translated_662_residues
MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSAILKGDIPEAEAILASIKPAG
VVSRADVVLPNQFQALRKSFIPERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIGMLVRSYNLNQQLLQRHYEEQNE
NAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIITCETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYD
FAVIANGVHEPWHAITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQFFDP
QQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDGSWDLPDGLIDRIECCGLMVTVGHWVL
EESCRLLAAWQERGIMLPLSVNLSVLQLMHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVR
VALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIA
QGFLFARPLPIEIFEESYLEEK
>Mature_662_residues
MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSAILKGDIPEAEAILASIKPAG
VVSRADVVLPNQFQALRKSFIPERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIGMLVRSYNLNQQLLQRHYEEQNE
NAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIITCETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYD
FAVIANGVHEPWHAITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQFFDP
QQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDGSWDLPDGLIDRIECCGLMVTVGHWVL
EESCRLLAAWQERGIMLPLSVNLSVLQLMHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVR
VALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIA
QGFLFARPLPIEIFEESYLEEK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI226510982, Length=662, Percent_Identity=99.2447129909366, Blast_Score=1338, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=428, Percent_Identity=30.8411214953271, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081921, Length=416, Percent_Identity=31.4903846153846, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI87081743, Length=237, Percent_Identity=35.8649789029536, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1790496, Length=251, Percent_Identity=37.0517928286853, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786507, Length=243, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI87081845, Length=250, Percent_Identity=31.2, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081980, Length=262, Percent_Identity=31.6793893129771, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788502, Length=253, Percent_Identity=31.2252964426877, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788849, Length=252, Percent_Identity=28.968253968254, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI87082096, Length=211, Percent_Identity=33.175355450237, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787055, Length=267, Percent_Identity=25.8426966292135, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787410, Length=153, Percent_Identity=32.6797385620915, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00672 HAMP [H]

EC number: NA

Molecular weight: Translated: 74522; Mature: 74522

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50885 HAMP ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIPERPVPVMVTRLFELPVQI
HHCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
SLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLVTIYLLLSLILTVAISWCINR
EECEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIGMLVRSYNLNQQLLQRHYEEQNE
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCC
NAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIITCETLRDTAGVLKEAQREILLLTLV
CEEECCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
EKLKSVLSPRMILAQISGYDFAVIANGVHEPWHAITLGQQVLTIMSERLPIERIQLRPHC
HHHHHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
SISVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQFFDPQQMEAAQKRLTEESDILNAL
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCHHHHHHHHH
ENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDGSWDLPDGLIDRIECCGLMVTVGHWVL
HCCCEEEEECCCEEECCCCEEEEEHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EESCRLLAAWQERGIMLPLSVNLSVLQLMHPNMVADMLELLTRYRIQPGTLILEVTESRR
HHHHHHHHHHHHCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
IDDPHAAVAILRPLRNAGVRVALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPED
CCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCC
EK
CC
>Mature Secondary Structure
MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIPERPVPVMVTRLFELPVQI
HHCCCCCHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
SLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLVTIYLLLSLILTVAISWCINR
EECEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIGMLVRSYNLNQQLLQRHYEEQNE
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCC
NAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIITCETLRDTAGVLKEAQREILLLTLV
CEEECCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
EKLKSVLSPRMILAQISGYDFAVIANGVHEPWHAITLGQQVLTIMSERLPIERIQLRPHC
HHHHHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
SISVAMFYGDLTAEQLYSRAISAAFTARHKGKNQIQFFDPQQMEAAQKRLTEESDILNAL
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCHHHHHHHHH
ENHQFAIWLQPQVEMTSGKLVSAEVLLRIQQPDGSWDLPDGLIDRIECCGLMVTVGHWVL
HCCCEEEEECCCEEECCCCEEEEEHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EESCRLLAAWQERGIMLPLSVNLSVLQLMHPNMVADMLELLTRYRIQPGTLILEVTESRR
HHHHHHHHHHHHCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
IDDPHAAVAILRPLRNAGVRVALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPED
CCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCC
EK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 10493123 [H]