Click here to switch to the map view.

The map label for this gene is gidA [H]

Identifier: 209400287

GI number: 209400287

Start: 4820283

End: 4822172

Strand: Reverse

Name: gidA [H]

Synonym: ECH74115_5177

Alternate gene names: 209400287

Gene position: 4822172-4820283 (Counterclockwise)

Preceding gene: 209400883

Following gene: 209399374

Centisome position: 86.54

GC content: 54.44

Gene sequence:

>1890_bases
ATGTTTTATCCGGATCCTTTTGACGTCATCATCATTGGCGGGGGTCATGCAGGCACCGAGGCCGCGATGGCTGCGGCGCG
TATGGGTCAACAGACTCTGCTTTTGACACACAATATCGACACTCTGGGGCAGATGAGCTGCAACCCGGCGATCGGCGGTA
TTGGGAAGGGACATCTGGTAAAAGAAGTGGATGCACTCGGCGGTCTGATGGCGAAAGCGATCGATCAGGCGGGTATCCAG
TTTAGGATACTAAACGCAAGCAAGGGACCGGCAGTTCGCGCTACCCGAGCTCAGGCGGATCGTGTGCTCTACCGTCAGGC
GGTACGTACGGCGCTGGAGAACCAACCGAACCTGATGATCTTCCAGCAGGCGGTTGAAGATCTTATTGTCGAAAACGATC
GTGTGGTCGGTGCTGTTACCCAAATGGGACTGAAGTTCCGTGCCAAAGCCGTCGTGCTCACCGTTGGGACGTTCCTCGAC
GGTAAAATTCATATCGGTCTGGATAACTACAGCGGTGGCCGTGCTGGTGATCCGCCGTCCATTCCGCTTTCTCGCCGTTT
GCGTGAACTTCCGCTGCGCGTTGGTCGTCTGAAAACCGGGACACCACCGCGTATTGATGCTCGAACCATCGACTTTAGCG
TGCTTGCGCAACAGCATGGCGATAACCCAATGCCGGTATTCTCTTTTATGGGCAATGCGTCCCAGCATCCACAGCAGGTG
CCGTGTTATATCACCCATACCAACGAGAAAACCCATGATGTGATCCGCAGTAACCTCGATCGTAGCCCAATGTACGCAGG
GGTGATCGAAGGTGTCGGCCCACGCTACTGCCCGTCGATCGAAGACAAAGTCATGCGCTTCGCCGACAGAAATCAGCATC
AGATCTTCCTTGAACCGGAAGGGCTGACTTCTAACGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTG
CAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTT
CTTCGATCCTCGCGACCTGAAACCGACGCTGGAGAGCAAGTTTATCCAGGGGCTGTTCTTTGCTGGTCAGATTAACGGCA
CTACCGGTTACGAAGAAGCCGCTGCGCAAGGTTTGCTGGCCGGTCTTAACGCTGCCCGTCTGTCTGATGACAAAGAAGGT
TGGGCTCCGGCGCGTTCTCAGGCCTATCTCGGCGTACTGGTTGATGACCTGTGCACTTTAGGAACCAAAGAACCGTATCG
TATGTTTACCTCGCGCGCAGAATATCGTCTGATGCTGCGCGAAGATAATGCGGATCTGCGTTTGACTGAAATCGGTCGTG
AACTGGGCCTGGTGGATGACGAACGTTGGGCGCGCTTTAACGAGAAACTTGAGAATATCGAGCGTGAGCGTCAGCGTCTG
AAATCGACCTGGGTAACCCCGTCGGCGGAAGCTGCAGCCGAAGTGAATGCTCACCTGACTGCGCCGCTTTCCCGTGAAGC
CAGTGGTGAAGATCTGCTGCGTCGTCCGGAAATGACTTATGAAAAATTAACCACGCTGACGCCGTTTGCCCCTGCGTTGA
CAGACGAACAGGCGGCGGAACAGGTTGAGATTCAGGTTAAATACGAAGGTTATATCGCGCGCCAGCAAGATGAGATCGAA
AAGCAGCTGCGTAACGAGAACACCTTGCTACCCGCGACACTGGATTACCGCCAGGTATCCGGTCTTTCTAACGAAGTGAT
CGCCAAACTTAACGATCACAAACCGGCCTCTATCGGCCAGGCTTCACGTATTTCTGGCGTCACGCCTGCGGCCATCTCCA
TTCTGCTGGTGTGGCTGAAAAAACAGGGTATGCTGCGTCGTAGCGCATAA

Upstream 100 bases:

>100_bases
TACCCAGGATCCCAGGTCTTTCTCAAGCCGACAAAGTTGAGTAGAATCCACGGCCCGGGCTTCAATCCATTTTCATACCG
CGTTATGCGAGGCAATCACC

Downstream 100 bases:

>100_bases
CGCATTAAAAATGCCTGGTAAGCCCCCGCTTACCAGGCAACGCATCAAGAACAGGTAATCACCGTGCTCAACAAACTCTC
CTTACTGCTGAAAGACGCAG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSDDKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA

Sequences:

>Translated_629_residues
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSDDKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA
>Mature_629_residues
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSDDKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=47.5435816164818, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=45.7317073170732, Blast_Score=516, Evalue=1e-146,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=44.7093889716841, Blast_Score=510, Evalue=1e-144,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=99.8410174880763, Blast_Score=1295, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=637, Percent_Identity=41.1302982731554, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=46.0420032310178, Blast_Score=524, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24658174, Length=643, Percent_Identity=47.1228615863142, Blast_Score=533, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69566; Mature: 69566

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
CCHHHHHHHCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSDDKEGWAPARSQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAE
HHHCCCCCHHHHHHCCCEEECCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCHHCCC
QVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEECEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGVTPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
CCHHHHHHHCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCHHHHHH
FIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSDDKEGWAPARSQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAE
HHHCCCCCHHHHHHCCCEEECCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCHHCCC
QVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEECEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGVTPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA