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The map label for this gene is narK

Identifier: 209400219

GI number: 209400219

Start: 1644799

End: 1646190

Strand: Direct

Name: narK

Synonym: ECH74115_1707

Alternate gene names: 209400219

Gene position: 1644799-1646190 (Clockwise)

Preceding gene: 209400976

Following gene: 209399352

Centisome position: 29.52

GC content: 53.45

Gene sequence:

>1392_bases
ATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCGGAAGATCCTGCGTTCTGGCA
ACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATTTCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGT
TGTTCAGCGCTGTTGCGGTGAACTTACCGAAAGTCGGTTTTAATTTTACGACCGATCAGCTATTTATGTTGACTGCGTTG
CCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGTGGTCGTCGCTGGACGGCGTT
CAGCACCGGTATTCTGATTATTCCTTGTGTCTGGCTGGGTTTTGCCGTGCAGGATACCTCCACGCCTTATAGCGTCTTCA
TCATCATCTCTCTGCTGTGCGGCTTTGCTGGCGCGAACTTCGCATCCAGTATGGCAAACATCAGCTTCTTCTTTCCGAAA
CAGAAGCAGGGTGGCGCGCTGGGCCTGAATGGTGGTCTGGGCAACATGGGCGTTAGCGTTATGCAGTTGGTTGCTCCGCT
GGTGGTATCACTGTCGATTTTCGCAGTATTTGGTAGCCAGGGCGTCAAACAGCCGGATGGGACTGAGCTGTATCTGGCGA
ATGCGTCCTGGATATGGGTGCCGTTCCTTGCCATCTTCACCATTGCGGCGTGGTTTGGCATGAACGATCTTGCTACCTCG
AAAGCCTCCATCAAGGAGCAGTTGCCGGTACTCAAACGGGGTCATCTGTGGATTATGAGCCTGCTGTATCTGGCAACCTT
CGGTTCCTTCATCGGCTTCTCCGCGGGCTTTGCGATGCTGTCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACG
CTTTCTTCGGGCCGTTTATTGGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTC
ACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAG
CTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTT
CCGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCA
ACCGACACGGCGGCGGCGTTGGGTTTCATCTCTGCGATTGGCGCGATTGGTGGCTTCTTTATCCCGAAAGCGTTCGGTAG
CTCGCTGGCATTAACGGGTTCGCCAGTCGGCGCAATGAAAGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCT
GGGCGGTATATGGTCGGCATTCTAAAAAATAA

Upstream 100 bases:

>100_bases
CAATGATAGATAAAGTTATCTTATCGTTTGATTTACATCAAATTGCCTTTAGCTACAGACACTAAGGTGGCAGACATCGA
AACGAGTATCAGAGGTGTCT

Downstream 100 bases:

>100_bases
ATCTTAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTT
ATATGGTTTATTTCGCCGGA

Product: nitrite extrusion protein 1

Products: NA

Alternate protein names: Nitrite facilitator 1

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTAL
PSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATS
KASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRV
TLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAA
TDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKK

Sequences:

>Translated_463_residues
MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTAL
PSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATS
KASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRV
TLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAA
TDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKK
>Mature_462_residues
SHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALP
SVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPKQ
KQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATSK
ASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVT
LVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAAT
DTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family

Homologues:

Organism=Escherichia coli, GI1787475, Length=463, Percent_Identity=100, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI1787743, Length=454, Percent_Identity=75.7709251101322, Blast_Score=658, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARK_ECOLI (P10903)

Other databases:

- EMBL:   X15996
- EMBL:   X69189
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X13360
- PIR:   S05239
- RefSeq:   AP_001851.1
- RefSeq:   NP_415741.1
- ProteinModelPortal:   P10903
- STRING:   P10903
- EnsemblBacteria:   EBESCT00000001420
- EnsemblBacteria:   EBESCT00000001421
- EnsemblBacteria:   EBESCT00000015606
- GeneID:   945783
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1214
- KEGG:   eco:b1223
- EchoBASE:   EB0636
- EcoGene:   EG10642
- eggNOG:   COG2223
- GeneTree:   EBGT00050000008841
- HOGENOM:   HBG307423
- OMA:   SPAGAMK
- ProtClustDB:   CLSK880005
- BioCyc:   EcoCyc:NARK-MONOMER
- Genevestigator:   P10903
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737
- TIGRFAMs:   TIGR00886

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49694; Mature: 49563

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e7a8db0)-; HASH(0x1f4fe1a4)-; HASH(0x1f5c535c)-; HASH(0x1e76f7b8)-; HASH(0x201c335c)-; HASH(0x1f9fcda0)-; HASH(0x1f18b9c8)-; HASH(0x1fdbe770)-; HASH(0x201b5e58)-; HASH(0x1fecd04c)-; HASH(0x1fedcab8)-; HASH(0x1fa24c78)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPCLLLAFCVWMLFSAVAV
CCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHH
NLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLG
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCEEEECCCCHHHHHHHHHC
FAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSV
EEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCCCCEEEECCCCCHHHHHH
MQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHH
KASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFI
HHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALARSAGGALSDRLGGTRVTLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFL
HHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHH
TAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFF
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
>Mature Secondary Structure 
SHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPCLLLAFCVWMLFSAVAV
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHH
NLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLG
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCEEEECCCCHHHHHHHHHC
FAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSV
EEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCCCCEEEECCCCCHHHHHH
MQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHH
KASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFI
HHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALARSAGGALSDRLGGTRVTLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFL
HHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHH
TAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFF
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKK
HHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652