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The map label for this gene is yfeA [H]

Identifier: 209400107

GI number: 209400107

Start: 3346543

End: 3348732

Strand: Reverse

Name: yfeA [H]

Synonym: ECH74115_3625

Alternate gene names: 209400107

Gene position: 3348732-3346543 (Counterclockwise)

Preceding gene: 209400602

Following gene: 209399083

Centisome position: 60.1

GC content: 48.95

Gene sequence:

>2190_bases
ATGTTTGTGGAGCATAACCTGATAAAAAATATCAAGATATTCACACTAGCGTTTACGCTCACCGTGGTACTTATTCAGCT
ATCCCGTTTTATTTCGCCACTTGCCATTATCCATTCCAGTTATATCTTTCTGGCGTGGATGCCACTGTGCGTAATGCTGT
CAATCTTGTTTATCTTTGGCTGGCGCGGTGTCGTTCCCGTTTTATGCGGGATGTTTTGCACCAATCTGTGGAACTTTCAT
CTCTCTTTTTTACAGACTGCGGTCATGCTCGGTAGCCAGACGTTTGTCGTGTTGTGTGCCTGCGCAATATTACGCTGGCA
GCTGGGGACGCGTTGGCGTTATGGATTGACCAGCCGATATGTCTGGCAACGTCTGTTCTGGCTTGGTTTGGTGACGCCGA
TCGGCATCAAATGCAGCATGTATCTTGTGGGGAGCTTCTTTGATTTCCCGCTAAAGATATCCACCTTTTTCGGCGATGCG
GATGCCATTTTCACGGTCGTTGATTTGCTAAGCCTTTTCACCGCTGTGCTGATTTACAACATGCTTTTCTACTATCTCAC
TCGCATGATTGTAAGTCCCCACTTTGCGCAGATATTGTGGCGCAGGGATATCGCTCCGTCGTTGGGCAAAGAGAAACGCG
CATTTACCTTAAGCTGGCTGGCAGCGTTAAGCGTGCTGCTACTTCTGTTGTGCACACCTTATGAAAACGACTTTATTGCC
GGTTACCTGGTACCCGTTTTCTTCATCATCTTTACCCTCGGGGTCGGTAAGCTTCGCTATCCGTTTTTAAATCTCACTTG
GGCTGTTTCAACGCTTTGCCTGCTGAATTACAACCAGAACTTTTTGCAAGGGGTGGAAACCGAATATTCGCTGGCATTTA
TTCTCGCGGTGCTGATTTCCTTTAGCGTTTGCCTGCTCTATATGGTGCGCATTTATCATCGCAGTGAATGGCTTAATCGC
CGCTGGCATTTGCAGGCGCTGACCGATCCGTTAACGCTCCTGCCCAACTTTCGTGCGTTGGAACAAGCGCCGGAGCAAGA
GGCGGGCAAGAGTTTTTGCTGCCTGCGCATTGATAATCTTGAGTTTATGAGTCGTCATTACGGGTTGATGATGCGTGTTC
ACTGTATCCGATCAATTTACCGTACGCTGCTGCCATTGATGCAGGAAAACGAAAAGTTGTATCAATTGCCGGGTAGTGAA
CTGCTGTTAGTGCTGAGCGGGCCTGAAACGGAAGGGCGACTCCAGCATATGGTTAACATCCTGAATAGTCGGCAAATTCA
CTGGAACAATACCGGGCTGGATATGGGCTATGGTGCTGCCTGGGGGCGTTTTGATGGAAATCAGGAAACCCTGCAACCCT
TGTTGGGGCAGTTAAGCTGGCTGGCGGAGCAATCCTGCGCACATCATCATGTGCTGGCGCTGGATAGCCGAGAGGAGATG
GTTTCCGGGCAGACCACTAAACAGGTGCTATTGCTGAATACCATTCGCACGGCGTTAGATCAGGGTGATTTGCTGCTCTA
CGCCCAGCCAATTCGCAACAAAGAGGGTGAAGGTTATGATGAGGTCCTCGCGCGACTGAAATATGACGGCGGCATTATGA
CCCCGGATAAGTTTCTGCCCCTTATTGCTCAGTTTAACCTTAGCGCGCGTTTTGATTTGCAAGTGCTGGAATCCTTGTTG
AAGTGGCTGGCAACACACCCTTGCGACAAAAAAGGCCCGCGCTTTTCAGTTAATTTAATGCCGCTCACGCTGCTGCAAAA
GAATATTGCCGGGCGGATTATTCGTCTATTTAAGCGTTATCACATCTCCCCGCAGGCGGTCATTCTTGAGATCACCGAGG
AGCAGGCGTTTTCTAACGCAGAAAGCAGCATGTACAACATCGAGCAGCTGCATAAGTTTGGTTTCCGGATTGCGATTGAT
GACTTTGGCACCGGCTATGCCAACTACGAACGGTTAAAGCGTTTGCAGGCTGATATCATCAAAATTGATGGCGTCTTTGT
GAAAGATATTGTCACGAATACGCTGGATGCGATGATTGTGAGATCAATTACCGATCTGGCGAAAGCGAAGTCATTGAGTG
TGGTCGCGGAGTTTGTCGAGACGCAACAGCAACAGGCGCTATTGCATAAGCTCGGGGTGCAATATCTGCAAGGGTATTTG
ATTGGTCGCCCGCAGCCATTAGCTGATTAA

Upstream 100 bases:

>100_bases
ATGAAAAATCGGCAACAGGATGGCCCCTGTTTGCTTCGCAATGCGAATAAACTTATTATTTGTGTGCCTGAAAACCCCGA
TCAGTGAGAGTAGTGTACTC

Downstream 100 bases:

>100_bases
CAGGCGTAAAAAAGCCGGGGAATTATCCCCGGCTTTTTTATGTATGGTCTTACAGCACCAGTGCTGCGATTGACGCAGAC
AGCACACTCACCAGGGTAGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFLAWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFH
LSFLQTAVMLGSQTFVVLCACAILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLSWLAALSVLLLLLCTPYENDFIA
GYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLNYNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNR
RWHLQALTDPLTLLPNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEKLYQLPGSE
LLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQETLQPLLGQLSWLAEQSCAHHHVLALDSREEM
VSGQTTKQVLLLNTIRTALDQGDLLLYAQPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLL
KWLATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSMYNIEQLHKFGFRIAID
DFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRSITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYL
IGRPQPLAD

Sequences:

>Translated_729_residues
MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFLAWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFH
LSFLQTAVMLGSQTFVVLCACAILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLSWLAALSVLLLLLCTPYENDFIA
GYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLNYNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNR
RWHLQALTDPLTLLPNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEKLYQLPGSE
LLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQETLQPLLGQLSWLAEQSCAHHHVLALDSREEM
VSGQTTKQVLLLNTIRTALDQGDLLLYAQPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLL
KWLATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSMYNIEQLHKFGFRIAID
DFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRSITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYL
IGRPQPLAD
>Mature_729_residues
MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFLAWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFH
LSFLQTAVMLGSQTFVVLCACAILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLSWLAALSVLLLLLCTPYENDFIA
GYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLNYNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNR
RWHLQALTDPLTLLPNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEKLYQLPGSE
LLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQETLQPLLGQLSWLAEQSCAHHHVLALDSREEM
VSGQTTKQVLLLNTIRTALDQGDLLLYAQPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLL
KWLATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSMYNIEQLHKFGFRIAID
DFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRSITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYL
IGRPQPLAD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87082096, Length=729, Percent_Identity=99.7256515775034, Blast_Score=1494, Evalue=0.0,
Organism=Escherichia coli, GI1788849, Length=711, Percent_Identity=29.676511954993, Blast_Score=310, Evalue=3e-85,
Organism=Escherichia coli, GI1790496, Length=235, Percent_Identity=31.4893617021277, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=32.2448979591837, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787541, Length=427, Percent_Identity=25.5269320843091, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787055, Length=294, Percent_Identity=29.9319727891156, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI226510982, Length=211, Percent_Identity=33.6492890995261, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081980, Length=242, Percent_Identity=30.1652892561983, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788502, Length=243, Percent_Identity=30.4526748971193, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI87081845, Length=235, Percent_Identity=30.2127659574468, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786507, Length=210, Percent_Identity=32.3809523809524, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081921, Length=262, Percent_Identity=27.8625954198473, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787410, Length=167, Percent_Identity=25.748502994012, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: NA

Molecular weight: Translated: 83571; Mature: 83571

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCC
>Mature Secondary Structure
MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFLAWMPLCVMLSILFIFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACAILRWQLGTRWRYGLTSRY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDADAIFTVVDLLSLFTAVLIYN
HHHHHHHHHHHCCCCCEEHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLSWLAALSVLLLLLCTPYENDFIA
HHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLNYNQNFLQGVETEYSLAFILAVLIS
HHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
FSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLLPNFRALEQAPEQEAGKSFCCLRIDNL
HHHHHHHHHHHHHHHHHHCCCEEHHHHCCHHHHCCCCHHHHCCCHHHCCCCEEEEEECCH
EFMSRHYGLMMRVHCIRSIYRTLLPLMQENEKLYQLPGSELLLVLSGPETEGRLQHMVNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCEEEEEECCCCHHHHHHHHHH
LNSRQIHWNNTGLDMGYGAAWGRFDGNQETLQPLLGQLSWLAEQSCAHHHVLALDSREEM
HHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
VSGQTTKQVLLLNTIRTALDQGDLLLYAQPIRNKEGEGYDEVLARLKYDGGIMTPDKFLP
HCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
LIAQFNLSARFDLQVLESLLKWLATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRY
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
HISPQAVILEITEEQAFSNAESSMYNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADII
CCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
KIDGVFVKDIVTNTLDAMIVRSITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGRPQPLAD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2201776 [H]