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The map label for this gene is ytfL

Identifier: 209400045

GI number: 209400045

Start: 5370535

End: 5371878

Strand: Reverse

Name: ytfL

Synonym: ECH74115_5735

Alternate gene names: 209400045

Gene position: 5371878-5370535 (Counterclockwise)

Preceding gene: 209398598

Following gene: 209398881

Centisome position: 96.41

GC content: 50.37

Gene sequence:

>1344_bases
ATGTTAAACAGTATTTTAGTCATACTCTGCTTGATCGCTGTAAGTGCGTTCTTCTCGATGTCTGAGATCTCACTTGCCGC
CTCACGCAAAATCAAACTTAAACTGCTGGCTGATGAAGGCAATATAAATGCCCAACGCGTTCTGAATATGCAGGAAAATC
CCGGCATGTTCTTTACCGTGGTCCAAATCGGTCTGAACGCAGTGGCGATTCTCGGCGGTATCGTCGGTGATGCGGCATTT
TCTCCAGCTTTTCACAGCCTGTTCTCCCGCTATATGTCGGCAGAGCTCTCTGAGCAACTGAGCTTTATTCTCTCTTTCTC
GTTAGTGACTGGCATGTTTATCCTGTTTGCGGATTTAACCCCGAAACGCATCGGTATGATTGCGCCAGAAGCTGTGGCTT
TGCGTATCATCAACCCGATGCGCTTCTGCCTGTACGTTTGCACCCCGCTGGTGTGGTTCTTCAACGGCCTGGCGAACATA
ATCTTCCGTATTTTCAAACTGCCAATGGTACGTAAAGATGACATCACTTCTGATGACATCTACGCGGTAGTGGAAGCCGG
TGCGCTGGCGGGCGTGTTACGTAAACAGGAACACGAGCTGATTGAAAACGTCTTTGAGCTGGAATCCCGTACCGTTCCGT
CTTCAATGACACCGCGTGAAAACGTGATTTGGTTTGATCTCCACGAAGATGAGCAAAGTCTGAAAAATAAGGTGGCGGAA
CATCCGCACTCTAAGTTCCTCGTCTGTAATGAAGATATTGACCACATCATCGGCTATGTCGATTCTAAAGACCTGCTGAA
CCGCGTGCTGGCTAACCAAAGCCTGGCACTGAACAGCGGCGTACAAATTCGCAACACGCTGATTGTGCCGGATACGTTAA
CCCTTTCAGAGGCGTTGGAAAGTTTTAAAACCGCAGGAGAAGACTTCGCGGTGATCATGAACGAGTACGCGCTGGTAGTG
GGGATCATCACCCTCAACGACGTGATGACCACGCTGATGGGCGATCTGGTCGGTCAGGGACTGGAAGAGCAGATTGTCGC
CCGTGATGAGAACTCATGGCTGATTGACGGCGGCACTCCGATTGACGACGTCATGCGCGTGCTGGATATTGACGAGTTCC
CGCAGTCGGGCAACTACGAAACCATCGGCGGCTTTATGATGTTTATGCTGCGTAAGATCCCGAAACGCACCGATTCGGTG
AAATTCGCCGGCTACAAATTTGAAGTGGTGGATATCGATAACTACCGCATCGACCAGCTGCTGGTGACCCGGATCGACAG
CAAGGCCACAGCCCTTTCGCCAAAACTGCCTGACGCTAAAGATAAAGAAGAAAGCGTCGCGTAA

Upstream 100 bases:

>100_bases
AATGCCGGTTCACTTTCTTCGAATCGGCTTTCAATGTGTATTTCACACAAATTAATCAACTTCCCCTTCCGAGGATCTGG
CCTGAAAGGTCGGATAAGAT

Downstream 100 bases:

>100_bases
CCCCCATAAACATCAACGGCTCCTGAATCAGGAGCCGTCTTATTACTGCATAGCACTTTTTACTGCATAGCACTTTGGTT
AAGCCATCTCTGTTTGCAGA

Product: putative transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINAQRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAF
SPAFHSLFSRYMSAELSEQLSFILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTPRENVIWFDLHEDEQSLKNKVAE
HPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLALNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVV
GIITLNDVMTTLMGDLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRKIPKRTDSV
KFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA

Sequences:

>Translated_447_residues
MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINAQRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAF
SPAFHSLFSRYMSAELSEQLSFILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTPRENVIWFDLHEDEQSLKNKVAE
HPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLALNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVV
GIITLNDVMTTLMGDLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRKIPKRTDSV
KFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA
>Mature_447_residues
MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINAQRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAF
SPAFHSLFSRYMSAELSEQLSFILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTPRENVIWFDLHEDEQSLKNKVAE
HPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLALNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVV
GIITLNDVMTTLMGDLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRKIPKRTDSV
KFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA

Specific function: Unknown

COG id: COG1253

COG function: function code R; Hemolysins and related proteins containing CBS domains

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains

Homologues:

Organism=Homo sapiens, GI310128564, Length=380, Percent_Identity=25, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1790664, Length=447, Percent_Identity=100, Blast_Score=912, Evalue=0.0,
Organism=Escherichia coli, GI145693175, Length=421, Percent_Identity=22.5653206650831, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082033, Length=238, Percent_Identity=32.3529411764706, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788119, Length=240, Percent_Identity=26.6666666666667, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786879, Length=262, Percent_Identity=24.0458015267176, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YTFL_ECO57 (P0AE47)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D86119
- PIR:   D91278
- RefSeq:   NP_290850.1
- RefSeq:   NP_313223.1
- ProteinModelPortal:   P0AE47
- PRIDE:   P0AE47
- EnsemblBacteria:   EBESCT00000027922
- EnsemblBacteria:   EBESCT00000059655
- GeneID:   913900
- GeneID:   959903
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5829
- KEGG:   ecs:ECs5196
- GeneTree:   EBGT00050000010862
- HOGENOM:   HBG673071
- OMA:   ENSWLVE
- ProtClustDB:   CLSK880871
- BioCyc:   ECOL83334:ECS5196-MONOMER
- InterPro:   IPR016169
- InterPro:   IPR000644
- InterPro:   IPR002550
- InterPro:   IPR005170
- Gene3D:   G3DSA:3.30.465.10
- SMART:   SM00116

Pfam domain/function: PF00571 CBS; PF03471 CorC_HlyC; PF01595 DUF21

EC number: NA

Molecular weight: Translated: 49763; Mature: 49763

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS51371 CBS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x139cc59c)-; HASH(0x13ca03b8)-; HASH(0x139e6880)-; HASH(0x139b858c)-; HASH(0x13c045dc)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINAQRVLNMQENPGMFFTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHEECCCCCCCEEEEH
VQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSFILSFSLVTGMFILFADLT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANIIFRIFKLPMVRKDDITSDDI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTPRENVIWFDLHEDEQSLKNKVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
HPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLALNSGVQIRNTLIVPDTLTLSEALE
CCCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCEEECCCCEEEEEEECCCCHHHHHHHH
SFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMGDLVGQGLEEQIVARDENSWLIDGGTP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCC
IDDVMRVLDIDEFPQSGNYETIGGFMMFMLRKIPKRTDSVKFAGYKFEVVDIDNYRIDQL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEEECCCCCHHHH
LVTRIDSKATALSPKLPDAKDKEESVA
HHHHHCCCHHCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINAQRVLNMQENPGMFFTV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHEECCCCCCCEEEEH
VQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSFILSFSLVTGMFILFADLT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANIIFRIFKLPMVRKDDITSDDI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTPRENVIWFDLHEDEQSLKNKVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
HPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLALNSGVQIRNTLIVPDTLTLSEALE
CCCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCEEECCCCEEEEEEECCCCHHHHHHHH
SFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMGDLVGQGLEEQIVARDENSWLIDGGTP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCC
IDDVMRVLDIDEFPQSGNYETIGGFMMFMLRKIPKRTDSVKFAGYKFEVVDIDNYRIDQL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEEECCCCCHHHH
LVTRIDSKATALSPKLPDAKDKEESVA
HHHHHCCCHHCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796