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The map label for this gene is leuE

Identifier: 209400042

GI number: 209400042

Start: 2388019

End: 2388657

Strand: Reverse

Name: leuE

Synonym: ECH74115_2524

Alternate gene names: 209400042

Gene position: 2388657-2388019 (Counterclockwise)

Preceding gene: 209395982

Following gene: 209396150

Centisome position: 42.87

GC content: 44.91

Gene sequence:

>639_bases
GTGTTCGCTGAATACGGGGTTCTGAATTACTGGACCTATCTGGTTGGGGCCATTTTTATTGTGTTGGTGCCAGGGCCAAA
TACCCTGTTTGTACTCAAAAATAGCGTCAGTAGCGGTATGAAAGGCGGTTATCTTGCGGCCTGCGGTGTATTTATTGGCG
ATGCGGTATTGATGTTTCTGGCATGGGCTGGAATGGCGACATTAATTAAGACCACCCCGATATTATTCAACATTGTACGT
TATCTTGGTGCGTTTTATTTGCTCTATCTGGGGAGTAAAATTCTTTACGCGACCCTGAAGGGTAAAAATAACGAGGCCAA
ATCCGATGAGCCCCAATACGGTGCTATTTTTAAACGCGCGTTAATTTTGAGCCTGACTAATCCGAAAGCCATTTTGTTCT
ATGTGTCGTTTTTCGTACAGTTTATCGATGTTAATGCCCCACATACGGGAATTTCATTCTTTATTCTGGCGACGACGCTG
GAACTGGTGAGTTTCTGCTATTTGAGCTTCCTGATTATATCTGGTGCTTTTGTCACGCAGTACATACGTACCAAAAAGAA
ACTGGCTAAAGTGGGCAACTCACTGATTGGTTTGATGTTCGTGGGTTTCGCTGCCCGACTGGCGACGCTGCAATCCTGA

Upstream 100 bases:

>100_bases
TCAGGTTAAAACCTGATTTTCTCCTTTCTAAGCCGCTACAGATTGGTTAGCATATTCACCTTTAATCGCGCATGATCGAA
AGATAATTAAAGAGGTTAAT

Downstream 100 bases:

>100_bases
TGCTTTCAGCCCGCGTTGTCGCGGGCTTCCCATCTATAATCCTCCCTGATTTTTCTCTGATATGGTGCTAAAAAGTAACC
AATAAATGGTATTTAAAAAG

Product: leucine export protein LeuE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVR
YLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS

Sequences:

>Translated_212_residues
MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVR
YLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS
>Mature_212_residues
MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVR
YLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS

Specific function: Exporter of leucine

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family

Homologues:

Organism=Escherichia coli, GI1788099, Length=212, Percent_Identity=98.5849056603774, Blast_Score=415, Evalue=1e-118,
Organism=Escherichia coli, GI1786522, Length=200, Percent_Identity=30.5, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994979, Length=203, Percent_Identity=27.0935960591133, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI48994978, Length=197, Percent_Identity=27.9187817258883, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUE_ECO57 (Q8XDS6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90942
- PIR:   G85790
- RefSeq:   NP_288234.1
- RefSeq:   NP_310534.1
- EnsemblBacteria:   EBESCT00000028958
- EnsemblBacteria:   EBESCT00000060213
- GeneID:   912651
- GeneID:   961771
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2841
- KEGG:   ecs:ECs2507
- GeneTree:   EBGT00050000008903
- HOGENOM:   HBG759042
- OMA:   GMLRSAW
- ProtClustDB:   PRK10958
- InterPro:   IPR001123

Pfam domain/function: PF01810 LysE

EC number: NA

Molecular weight: Translated: 23290; Mature: 23290

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2168f560)-; HASH(0x2152dd88)-; HASH(0x211bbca4)-; HASH(0x20f54e24)-; HASH(0x2091a1e8)-; HASH(0x1f8fec04)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFL
CCCCCHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
AWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCHHHHHHHHH
LILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
HHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFL
CCCCCHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
AWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGKNNEAKSDEPQYGAIFKRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCHHHHHHHHH
LILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
HHHEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796