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The map label for this gene is oppA [H]

Identifier: 209400038

GI number: 209400038

Start: 1665477

End: 1667153

Strand: Direct

Name: oppA [H]

Synonym: ECH74115_1729

Alternate gene names: 209400038

Gene position: 1665477-1667153 (Clockwise)

Preceding gene: 209399730

Following gene: 209398544

Centisome position: 29.89

GC content: 47.7

Gene sequence:

>1677_bases
ATGCTGGTTAATACCAGTAATTATAATGAGGGAGTCCAAAAAACAATGACCAACATCACCAAGAGAAGTTTAGTAGCAGC
TGGCGTTCTGGCTGCGCTAATGGCAGGGAATGTCGCGCTGGCAGCTGATGTACCCGCAGGCGTCACACTGGCGGAAAAAC
AAACGCTGGTACGTAACAATGGTTCAGAAGTTCAGTCATTAGATCCGCACAAAATTGAAGGTGTTCCGGAGTCTAATATC
AGCCGAGACCTGTTTGAAGGCTTACTGGTCAGCGATCTTGACGGCCATCCGGCACCTGGCGTCGCTGAATCCTGGGATAA
TAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACT
TTGTGTATAGCTGGCAACGTTCTGTTGATCCGAACACCGCTTCTCCGTATGCCAGTTATCTGCAATATGGGCATATCGCC
GGTATTGATGAAATTCTTGAAGGGAAAAAACCGATTACCGATCTCGGCGTGAAAGCTATTGATGATCACACATTAGAAGT
CACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCG
AGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAAC
GAACGAATCGTTCTTGAACGCAGCCCAACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCCAT
TGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACTTATAACAACATGCCGATCGAATTGT
TCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTATTATTACGAAATTAACAAC
CAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTGGGTATGGACCGCGATATCATTGTTAATAAAGT
GAAAGCGCAGGGCGACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCGGAATGGT
TTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTG
ACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACAT
TGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCC
GTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTGTCGAACAGCTCGATGAATACCGCG
CATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTA
CACTAAAGCAGAACAACAGCTGGATAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAAC
CGTGGGTTGGTGGTTATACCGGCAAAGATCCGCTGGATAATACCTACACCCGGAATATGTACATTGTGAAGCACTGA

Upstream 100 bases:

>100_bases
ACCAGAATAAATAAAGTCGGTGATAGCAATCCGTAACGATAAAGTAACCTGACAGCAGAAAGTCTCCGAGCCTGTGCAGG
GTCCCAATCCGGGATTACAC

Downstream 100 bases:

>100_bases
TGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTA
GGGCAATGTTAAAATTTATT

Product: oligopeptide ABC transporter, periplasmic oligopeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MLVNTSNYNEGVQKTMTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRNNGSEVQSLDPHKIEGVPESNI
SRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHLRKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIA
GIDEILEGKKPITDLGVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVN
ERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDEVHVDPYLCTYYYEINN
QKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPL
TINLLYNTSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNTA
HYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH

Sequences:

>Translated_558_residues
MLVNTSNYNEGVQKTMTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRNNGSEVQSLDPHKIEGVPESNI
SRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHLRKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIA
GIDEILEGKKPITDLGVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVN
ERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDEVHVDPYLCTYYYEINN
QKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPL
TINLLYNTSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNTA
HYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH
>Mature_558_residues
MLVNTSNYNEGVQKTMTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRNNGSEVQSLDPHKIEGVPESNI
SRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHLRKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIA
GIDEILEGKKPITDLGVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVN
ERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDEVHVDPYLCTYYYEINN
QKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPL
TINLLYNTSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNTA
HYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=99.4475138121547, Blast_Score=1117, Evalue=0.0,
Organism=Escherichia coli, GI87081878, Length=531, Percent_Identity=48.0225988700565, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=44.3396226415094, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1787052, Length=515, Percent_Identity=26.2135922330097, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787762, Length=518, Percent_Identity=26.2548262548263, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789966, Length=488, Percent_Identity=25.4098360655738, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789887, Length=500, Percent_Identity=25.2, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787551, Length=549, Percent_Identity=23.4972677595628, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62656; Mature: 62656

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVNTSNYNEGVQKTMTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRNN
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEHHHHHHHHCC
GSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHLRKD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECC
AKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDLGVKAI
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCHHHHCCEEE
DDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVN
CCCEEEEEECCCCCHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCCEEEHHHHCC
ERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKE
CEEEEECCCCCCCCHHHHHHHHHEECCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
IPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTP
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHEEHHCCCCCCCCCCCC
PYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYNTSDLHKKLAIAAS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHH
SLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNTA
HHHHHCCCCEEEEECHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCC
HYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEECCCCCCCC
GKDPLDNTYTRNMYIVKH
CCCCCCCCEECCEEEEEC
>Mature Secondary Structure
MLVNTSNYNEGVQKTMTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRNN
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEHHHHHHHHCC
GSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHLRKD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECC
AKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDLGVKAI
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCHHHHCCEEE
DDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVN
CCCEEEEEECCCCCHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCCEEEHHHHCC
ERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKE
CEEEEECCCCCCCCHHHHHHHHHEECCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
IPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTP
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHEEHHCCCCCCCCCCCC
PYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYNTSDLHKKLAIAAS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHH
SLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNTA
HHHHHCCCCEEEEECHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCC
HYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVGGYT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEECCCCCCCC
GKDPLDNTYTRNMYIVKH
CCCCCCCCEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]