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The map label for this gene is ntpA [H]

Identifier: 209399929

GI number: 209399929

Start: 2456404

End: 2456913

Strand: Reverse

Name: ntpA [H]

Synonym: ECH74115_2601

Alternate gene names: 209399929

Gene position: 2456913-2456404 (Counterclockwise)

Preceding gene: 209396974

Following gene: 209400343

Centisome position: 44.09

GC content: 51.76

Gene sequence:

>510_bases
ATGAGCATAGATAACTATGTGAATGGGATGAGCGAAGGCAGTCAACGAAGAGGCAGCGTGAAGGATAAAGTGTATAAGCG
TCCCGTTTCGATCTTAGTGGTCATCTACGCACAAGATACGAAACGGGTGCTGATGTTGCAGCGGCGTGATGACCCCGATT
TCTGGCAGTCGGTAACCGGCAGCGTGGAAGAGGGTGAAACCGCGCCGCAAGCTGCCATGCGCGAAGTAAAGGAAGAGGTC
ACCATTGATGTTGTCGCTGAACAACTGACCTTAATTGACTGTCAGCGCACGGTAGAGTTTGAAATTTTTTCACATTTACG
TCATCGCTATGCGCCGGGCGTGACGCGTAATACGGAATCATGGTTCTGTCTTGCGCTTCCGCATGAGCGGCAGATCGTTT
TCACTGAACATCTGGCTTACAAGTGGCTTGATGCGCCTGCTGCGGCGGCGCTCACTAAGTCCTGGAGCAACCGGCAGGCG
ATTGAACAGTTTGTAATTAACGCTGCCTGA

Upstream 100 bases:

>100_bases
GCTGAGCATTCAGGTTGTGAAGAAGGCTGAGAATAACTGATATGACTCAAATACACGAAATCATTCGCGTTGCATCGAGG
CGGCAACTGAGTGAACTCCC

Downstream 100 bases:

>100_bases
ACAGGCAGGGCTATCTGGAGATATTTTTATGGCAGGTCATAGTAAATGGGCCAACACCAGACATCGTAAAGCTGCGCAGG
ATGCTAAGCGCGGTAAAATC

Product: dATP pyrophosphohydrolase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MSIDNYVNGMSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEV
TIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQA
IEQFVINAA

Sequences:

>Translated_169_residues
MSIDNYVNGMSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEV
TIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQA
IEQFVINAA
>Mature_168_residues
SIDNYVNGMSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVT
IDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAI
EQFVINAA

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=150, Percent_Identity=99.3333333333333, Blast_Score=311, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 19344; Mature: 19213

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDNYVNGMSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTG
CCCCHHHCCCCCCHHHCCCHHHHHHHCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCC
SVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTES
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
EEEEECCCCCEEEEHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SIDNYVNGMSEGSQRRGSVKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTG
CCCHHHCCCCCCHHHCCCHHHHHHHCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCC
SVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTES
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
EEEEECCCCCEEEEHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]