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The map label for this gene is folM [H]

Identifier: 209399782

GI number: 209399782

Start: 2187072

End: 2187794

Strand: Direct

Name: folM [H]

Synonym: ECH74115_2317

Alternate gene names: 209399782

Gene position: 2187072-2187794 (Clockwise)

Preceding gene: 209398615

Following gene: 209400881

Centisome position: 39.25

GC content: 50.76

Gene sequence:

>723_bases
ATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCAT
TAATCAAAAGCAACCGGTGATTGTCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGT
GTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGT
GCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCA
GATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATA
TCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGAT
AATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAA
TGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCATTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGA
TCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGT
TAA

Upstream 100 bases:

>100_bases
AATGGTGCTGATTGGCATGCTGTTAATTGCCTCAGTTCCGGCGACCTGGATGCTGGTGGGATAACGTTCATCCCATCGTT
GGATAGCAAAGGAGATAACG

Downstream 100 bases:

>100_bases
TGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAA
GCGCATCATTCCGGTGAAAT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLR
AILHNASAWMAEKPGAPLADVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR

Sequences:

>Translated_240_residues
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLR
AILHNASAWMAEKPGAPLADVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
>Mature_239_residues
GKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRA
ILHNASAWMAEKPGAPLADVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDN
MTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR

Specific function: Catalyzes the reduction of dihydrofolate to tetrahydrofolate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787891, Length=240, Percent_Identity=100, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=26.2295081967213, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 26349; Mature: 26217

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFS
CCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCHHHEECCCC
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFS
CCCCCCEEEEECCCCEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCHHHEECCCC
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]