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The map label for this gene is sapA [H]

Identifier: 209399749

GI number: 209399749

Start: 1830415

End: 1832034

Strand: Reverse

Name: sapA [H]

Synonym: ECH74115_1935

Alternate gene names: 209399749

Gene position: 1832034-1830415 (Counterclockwise)

Preceding gene: 209396371

Following gene: 209400983

Centisome position: 32.88

GC content: 54.38

Gene sequence:

>1620_bases
TTGGTGATTGCTGGACTTGTGAGTGGTCAGGCAATCGCCGCGCCTGAATCCCCCCCGCATGCTGATATCCGCGACAGCGG
TTTTGTCTATTGCGTCAGCGGGCAAGTCAACACCTTTAACCCATCCAAAGCGAGCAGTGGGTTAATTGTCGATACCCTTG
CCGCCCAGTTTTATGATCGACTGCTGGATGTCGATCCCTATACCTATCGCCTGATGCCAGAACTTGCCGAAAGCTGGGAA
GTACTCGACAACGGCGCGACCTATCGCTTCCACCTGCGTCGCGATGTCCCGTTTCAAAAAACCGCCTGGTTTACTCCCAC
TCGCAAAATGAATGCCGACGATGTGGTGTTTACCTTCCAGCGAATTTTTGACCGCAACAACCCGTGGCATAACGTCAACG
GCAGCAACTTCCCCTACTTCGACAGCCTGCAATTTGCCGATAACGTGAAAAGCGTCCGTAAACTGGATAATCATACCGTT
GAGTTCCGTCTGGCTCAGCCGGATGCTTCTTTTTTGTGGCACCTCGCAACCCATTATGCTTCGGTCATGTCGGCAGAATA
TGCCCGGAAGTTAGAGAAAGAAGATCGCCAGGAGCAACTCGACCGTCAACCGGTCGGCACTGGACCGTATCAGTTGTCGG
AATACCGCGCCGGGCAATTTATTCGCCTACAACGTCATGATGACTTCTGGCGCGGTAAACCGTTAATGCCGCAGGTGGTG
GTGGATTTAGGCTCCGGCGGCACCGGACGTCTGTCGAAACTCCTGACCGGGGAATGCGACGTTCTGGCCTGGCCTGCTGC
CAGCCAGCTATCCATTTTGCGTGACGACCCGCGCTTGCGTTTAACGCTGCGTCCTGGGATGAACGTCGCCTATCTGGCAT
TTAACACCGCCAAACCGCCGCTAAATAATCCCGCTGTCCGCCATGCGCTGGCACTGGCGATTAATAACCAGCGCCTGATG
CAATCCATCTATTATGGTACGGCTGAAACGGCGGCCTCTATTTTACCGCGCGCCTCGTGGGCCTATGACAACGAGGCTAA
AATTACTGAATACAATCCGGCGAAATCGCGCGAACAGTTGAAGTTGTTGGGGCTGGAAAATTTAACGCTGAAACTGTGGG
TGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACTGCCGAACTGATTCAGGCGGATATGGCGCAGGTTGGC
GTAAAAGTGGTGATTGTGCCGGTAGAAGGTCGCTTTCAGGAGGCGCGGTTGATGGATATGAGCCATGATCTGACGTTATC
CGGTTGGGCGACGGACAGTAACGACCCGGACAGTTTCTTCCGTCCGTTACTGAGCTGCGCGGCAATTCATTCACAGACCA
ACCTCGCCCACTGGTGCGATCCGAAATTCGACAGCGTGTTGCGTAAGACGCTCTCCTCGCAGCAGCTGGCGGCGCGTATT
GAAGCCTATGACGAAGCGCAGAGTATTCTGGCGCAGGAATTGCCCATTTTGCCGCTGGCGTCGTCATTGCGTTTGCAGGC
CTATCGGTACGATATCAAAGGTCTGGTACTTAGCCCGTTTGGTAACGCCTCCTTTGCTGGGGTGTATCGCGAGAAACAGG
ATGAGGTGAAAAAACCATGA

Upstream 100 bases:

>100_bases
CCTGAACACAAACGGTAATGGAAAATGTCATTAAATATTAGTAAGATAGCCTCTTTACATAAAATCCCTTAATATTATGC
GCCAGGTATTATCGTCTCTT

Downstream 100 bases:

>100_bases
TTATCTTCACCTTACGCCGCATTTTGTTATTGATTGTCACCTTGTTCCTGCTGACCTTCGTTGGCTTCAGCTTGAGCTAT
TTCACTCCACACGCGCCGTT

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWE
VLDNGATYRFHLRRDVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTV
EFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRGKPLMPQVV
VDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLM
QSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVG
VKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARI
EAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP

Sequences:

>Translated_539_residues
MVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWE
VLDNGATYRFHLRRDVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTV
EFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRGKPLMPQVV
VDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLM
QSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVG
VKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARI
EAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP
>Mature_539_residues
MVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWE
VLDNGATYRFHLRRDVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTV
EFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRGKPLMPQVV
VDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLM
QSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVG
VKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARI
EAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=539, Percent_Identity=99.2578849721707, Blast_Score=1108, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=512, Percent_Identity=35.546875, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787052, Length=510, Percent_Identity=27.4509803921569, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1787762, Length=467, Percent_Identity=23.1263383297645, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787495, Length=529, Percent_Identity=24.7637051039698, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=25, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789397, Length=524, Percent_Identity=23.2824427480916, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60680; Mature: 60680

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDR
CEEEEEECCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
LLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQKTAWFTPTRKMNADDVVFTFQ
HHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHH
RIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTVEFRLAQPDASFLWHLATHYA
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
SVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRGKPLMPQVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCHHHE
VDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPP
EECCCCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCCEEEEECCCCCEEEEEEECCCCC
LNNPAVRHALALAINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQL
CCCHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHE
KLLGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDM
EEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCC
SHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP
HHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDR
CEEEEEECCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
LLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQKTAWFTPTRKMNADDVVFTFQ
HHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHH
RIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTVEFRLAQPDASFLWHLATHYA
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
SVMSAEYARKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRGKPLMPQVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCHHHE
VDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPP
EECCCCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCCEEEEECCCCCEEEEEEECCCCC
LNNPAVRHALALAINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQL
CCCHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHE
KLLGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDM
EEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCC
SHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP
HHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]