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The map label for this gene is ogt [H]

Identifier: 209399746

GI number: 209399746

Start: 1874895

End: 1875410

Strand: Reverse

Name: ogt [H]

Synonym: ECH74115_1982

Alternate gene names: 209399746

Gene position: 1875410-1874895 (Counterclockwise)

Preceding gene: 209399301

Following gene: 209399588

Centisome position: 33.66

GC content: 53.1

Gene sequence:

>516_bases
ATGCTGAGATTACTTGAAGAAAAAATTGCCACACCACTGGGTCCACTGTGGGTGATTTGCGATGAGCAATTTCGCCTGCG
GGCGGTTGAATGGGAAGAGTACAGCGAACGCATGGTGCAGCTGCTGGACATCCATTATCGCAAAGAAGGCTATGAGCGCA
TTTCTGCCACCAATCCAGGCGGTTTAAGCGACAAGCTTCGTGAATATTTTGCCGGTAATCTTAGCATTATTGATACGCTT
CCCACTGCTACGGGGGGGACGCCATTTCAGCGCGAAGTCTGGAAAACACTACGCACTATCCCCTGCGGGCAGGTAATGCA
TTACGGCGAACTGGCTGAGCAATTGGGCCGTCCTGGCGCGGCGCGTGCCGTTGGTGCGGCAAACGGATCGAATCCCATCA
GCATCGTCGTACCTTGCCATCGGGTTATTGGCCGAAACGGCACCATGACCGGATATGCAGGCGGAGTTCAGCGAAAAGAG
TGGTTATTGCGCCACGAAGGTTATCTTTTGCTGTAA

Upstream 100 bases:

>100_bases
ATCTTTTTGGTGGTATGGCTGCTGATGTTGCTGGCGTGGTATCTTGTGGGCCTGCCGATAGGTCCGGGTATTTACCCACG
TTTGTCTTAAGAGAGAACGG

Downstream 100 bases:

>100_bases
ACATTAAACAATTTGTGCCAGCTTGTTCACACTTTTATGTAAAGTTACCCTTAACAACTTAAGGGTTTTCAAATAGATAG
ACATATATTTACATCTAATA

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Sequences:

>Translated_171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL
>Mature_171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=83, Percent_Identity=49.3975903614458, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787596, Length=171, Percent_Identity=99.4152046783626, Blast_Score=350, Evalue=2e-98,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=50.561797752809, Blast_Score=103, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=93, Percent_Identity=38.7096774193548, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=93, Percent_Identity=38.7096774193548, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=48.1481481481481, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137554, Length=105, Percent_Identity=40.9523809523809, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19180; Mature: 19180

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
CCCHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGELAEQLGRPGA
CCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
HHEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
CCCHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGELAEQLGRPGA
CCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
HHEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]