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The map label for this gene is ebgR [H]

Identifier: 209399661

GI number: 209399661

Start: 4064080

End: 4065063

Strand: Direct

Name: ebgR [H]

Synonym: ECH74115_4389

Alternate gene names: 209399661

Gene position: 4064080-4065063 (Clockwise)

Preceding gene: 209397233

Following gene: 209400384

Centisome position: 72.94

GC content: 51.42

Gene sequence:

>984_bases
ATGGCAACACTAAAAGACATCGCAATCGAAGCTGGCGTATCCCTGGCGACAGTATCCAGGGTCTTAAATGACGATCCGAC
ATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGCGCCCGTA
AACTACAGACAGGCGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCT
TACTATCTGGCGATCCGCCACGGCATTGAAACTCAGTGCGAAAAGCTGGCGATCGAACTGACCAACTGTTATGAACACAG
CGGATTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCG
CGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGC
ATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGG
CAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCG
GCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTT
GCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCGATTCATGAACGTGGTCTGAACATCCCACAGGATATTTCGCTTAT
CAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAAATGATGGGAA
GTCAGGGCGTAAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTA
AAACTGCGCGGCACGACCCGTTAA

Upstream 100 bases:

>100_bases
CAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGTGACCCGATAAGCACTTCCTCTACAATGGGGGCGCACATCAG
GGAAAGTAAAAAAGGTAAAC

Downstream 100 bases:

>100_bases
ATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGGCAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGC
ATTTACCCGCTACTTTTTAG

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLAIELTNCYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR

Sequences:

>Translated_327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLAIELTNCYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR
>Mature_326_residues
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPY
YLAIRHGIETQCEKLAIELTNCYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARI
SKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFVA
SDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLK
LRGTTR

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=99.6941896024465, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=28.1437125748503, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=26.4984227129338, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=26.7647058823529, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788474, Length=347, Percent_Identity=25.6484149855908, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786540, Length=344, Percent_Identity=27.0348837209302, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=27.0627062706271, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36225; Mature: 36094

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYEHSGLPDIKNVTGILI
CCCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
VGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
GGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC
>Mature Secondary Structure 
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYEHSGLPDIKNVTGILI
CCCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
VGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
GGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]