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The map label for this gene is narU [H]

Identifier: 209399656

GI number: 209399656

Start: 1976508

End: 1977896

Strand: Reverse

Name: narU [H]

Synonym: ECH74115_2079

Alternate gene names: 209399656

Gene position: 1977896-1976508 (Counterclockwise)

Preceding gene: 209397006

Following gene: 209398888

Centisome position: 35.5

GC content: 49.32

Gene sequence:

>1389_bases
ATGGCACTGCAAAATGAGAAAAATAGTCGTTATCTTTTACGCGACTGGAAACCAGAAAATCCGGCCTTCTGGAAAAATAA
AGGAAAACATATTGCTCGAAGAAACCTCTGGATATCAGTCAGTTGTCTACTTCTTGCCTTCTGTGTCTGGATGCTATTTA
GCGCAGTTACCGTTAATCTCAATAAAATCGGTTTTAATTTCACTACCGATCAACTCTTTTTATTAACCGCATTACCCTCC
GTTTCTGGCGCATTATTACGTGTCCCCTACTCCTTTATGGTGCCTATATTCGGTGGACGTCGATGGACGGTTTTTAGTAC
TGCAATCCTGATTATTCCTTGCGTCTGGCTCGGAATTGCCGTGCAGAATCCGAATACTCCTTTTGGGATATTTATCGTTA
TCGCTTTGCTATGCGGTTTTGCAGGTGCGAACTTTGCTTCGAGCATGGGCAATATCAGTTTCTTCTTTCCAAAAGCCAGG
CAAGGTAGCGCTCTTGGGATTAATGGCGGATTAGGAAACTTAGGTGTAAGTGTAATGCAGCTGGTTGCACCGCTGGTCAT
TTTTGTACCCGTATTTGCCTTTCTCGGCGTCAATGGAGTACCGCAGGCCGACGGTTCGGTGATGTCGCTGGCGAATGCCG
CATGGATTTGGGTACCGCTACTAGCGATTGCCACGATCGCCGCCTGGTCAGGGATGAATGATATCGCCAGTTCACGCGCG
TCAATTGCCGACCAGCTCCCTGTCTTACAACGCCTGCATCTCTGGCTGCTGAGCCTGCTTTACCTTGCCACCTTCGGTTC
GTTTATCGGTTTTTCTGCGGGTTTCGCCATGCTGGCAAAAACCCAGTTCCCGGATGTGAATATTCTGCGCCTGGCGTTCT
TTGGCCCATTTATCGGTGCCATCGCGCGGTCGGTGGGTGGTGCTATTTCCGATAAATTCGGCGGCGTGCGGGTGACGTTG
ATCAACTTTATTTTTATGGCGATTTTCAGCGCCCTGCTGTTCCTTACCTTACCGGGCACAGGCTCCGGTAACTTCATCGC
CTTTTACGCCGTATTTATGGGGCTGTTTCTGACCGCGGGTCTGGGAAGTGGTTCTACTTTCCAGATGATCGCCGTCATCT
TTCGCCAGATAACCATTTATCGGATGAAGATGAAAGGCGGTAGTGATGAGCAAGCTCAGAGAGAAGCCGTCACCGAAACG
GCGGCGGCTCTGGGCTTTATCTCAGCCATCGGCGCAGTGGGCGGCTTTTTTATTCCGCAGGCATTTGGCATGTCACTCAA
TATGACCGGCTCTCCAGTGGGCGCGATGAAAGTGTTTTTAATCTTCTACATCGTTTGTGTGCTACTGACCTGGCTGGTTT
ATGGTCGGCGGAAGTTCAGCCAAAAATAA

Upstream 100 bases:

>100_bases
CACTTCACCCATTTCATCCTTAATTCTAATAACTGGCTATTGCGCCTTACCTCCTGTTTTGTCTTTTTTTCATTACCAAT
GTGTACATGTGAGGAACAAT

Downstream 100 bases:

>100_bases
GAGATCGATGCCTGATGTACAACCGTGTCGCATCCGACAAGGCATCAGATCCCAATTCATTCCTGGAGCAGGAGTCATGT
CATGAGTAAACTTTTGGATC

Product: nitrite extrusion protein 2

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPS
VSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTL
INFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQAQREAVTET
AAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK

Sequences:

>Translated_462_residues
MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPS
VSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTL
INFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQAQREAVTET
AAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
>Mature_461_residues
ALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSV
SGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKARQ
GSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRAS
IADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLI
NFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQAQREAVTETA
AALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=98.9177489177489, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI1787475, Length=454, Percent_Identity=75.5506607929515, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49969; Mature: 49838

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNL
CCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
NKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIA
HHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEE
VQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKARQGSALGINGGLGNLGVSVMQ
ECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
LVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAG
HHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHC
LGSGSTFQMIAVIFRQITIYRMKMKGGSDEQAQREAVTETAAALGFISAIGAVGGFFIPQ
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
HHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNL
CCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
NKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIA
HHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEE
VQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKARQGSALGINGGLGNLGVSVMQ
ECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
LVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAG
HHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHC
LGSGSTFQMIAVIFRQITIYRMKMKGGSDEQAQREAVTETAAALGFISAIGAVGGFFIPQ
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
HHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2233673; 7747940 [H]