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The map label for this gene is dapF

Identifier: 209399378

GI number: 209399378

Start: 4891535

End: 4892359

Strand: Direct

Name: dapF

Synonym: ECH74115_5248

Alternate gene names: 209399378

Gene position: 4891535-4892359 (Clockwise)

Preceding gene: 209398527

Following gene: 209398950

Centisome position: 87.79

GC content: 54.67

Gene sequence:

>825_bases
ATGCAGTTCTCGAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCACC
GGAGCTGATTCGTCGCCTAGCTGATCGGCACCTGGGGGTAGGGTTTGACCAACTGCTGGTGGTTGAGCCGCCGTATGATC
CTGAACTGGATTTTCACTATCGCATTTTCAATGCTGATGGCAGTGAAGTGGCGCAGTGCGGCAACGGCGCACGCTGCTTT
GCCCGTTTTGTGCGTCTGAAAGGGCTGACCAATAAGCGCGATATCCGCGTCAGCACCGCCAACGGGCGGATGGTTCTTAC
CGTCACCGATGATGATCTGGTCCGCGTAAATATGGGCGAACCCAACTTCGAACCTTCTGCCGTGCCGTTTCGCGCTAACA
AAGCGGAAAAGACCTATATTATGCGCGCCGCCGAGCAGACAATCTTATGCGGCGTGGTGTCGATGGGAAATCCACATTGC
GTGATTCAGGTCGATGATGTCGATACTGCGGCGGTAGAAACACTGGGGCCTGTTCTGGAAAGCCACGAGCGTTTTCCGGA
GCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAA
CCCAGGCCTGCGGCAGCGGCGCATGTGCGGCGGTTGCAGTAGGGATTCAGCAAGGTTTGCTGGCCGAAGAAGTACGCGTG
GAACTCCCAGGCGGTCGTCTTGATATCGCCTGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGT
CTACGACGGATTTATTCATCTATGA

Upstream 100 bases:

>100_bases
ATCCACGGTGCCGGATAAAAACGATCGCGCCACTGGCGATGGTCCATCCCAGGTGAATTACTAAAAGTCAGTTTCTGTAC
CCGCGTGATTGGAGTAAATG

Downstream 100 bases:

>100_bases
AGCAACCAGGGGAAGAACTGCAGGAAACACTCACGGAGCTTGATGACCGGGTGGTTGTCGATTATCTGATTAAAAATCCT
GAGTTTTTTATCCGCAATGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
>Mature_274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=100, Blast_Score=566, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ECO24 (A7ZU14)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465293.1
- ProteinModelPortal:   A7ZU14
- SMR:   A7ZU14
- STRING:   A7ZU14
- EnsemblBacteria:   EBESCT00000021751
- GeneID:   5589486
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4328
- eggNOG:   COG0253
- GeneTree:   EBGT00050000011505
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   ECOL331111:ECE24377A_4328-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30209; Mature: 30209

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVETLGPVLE
CCCCCCCCCEECCCCCHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
SHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
HHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHEEE
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
ECCCCEEEEEEECCCCEEEEECCEEEEECCEEEC
>Mature Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVETLGPVLE
CCCCCCCCCEECCCCCHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
SHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV
HHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHEEE
ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL
ECCCCEEEEEEECCCCEEEEECCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA