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The map label for this gene is garR

Identifier: 209399219

GI number: 209399219

Start: 4114185

End: 4115069

Strand: Reverse

Name: garR

Synonym: ECH74115_4437

Alternate gene names: 209399219

Gene position: 4115069-4114185 (Counterclockwise)

Preceding gene: 209397366

Following gene: 209399632

Centisome position: 73.85

GC content: 52.77

Gene sequence:

>885_bases
ATGAAAGTTGGTTTTATTGGCCTGGGGATTATGGGTAAACCAATGAGTAAAAACCTTCTGAAAGCAGGTTACTCGCTGGT
GGTTGCTGACCGTAACCCAGAAGCTATTGCTGACGTGATTGCTGCAGGTGCAGAAACAGCTTCTACGGCTAAAGCGATCG
CTGAACAGTGCGACGTCATCATAACCATGCTGCCAAACTCCCCTCATGTGAAAGAGGTGGCGCTGGGTGAGAATGGCATT
ATTGAAGGCGCGAAGCCAGGTACGGTATTGATCGATATGAGTTCTATCGCACCGCTGGCAAGCCGTGAAATCAGCGAAGC
GCTGAAAGCGAAAGGCATTGATATGCTGGATGCTCCGGTGAGCGGCGGTGAACCGAAAGCCATCGACGGTACGCTGTCAG
TGATGGTAGGCGGCGACAAGGCTATTTTCGACAAATACTATGATTTGATGAAAGCGATGGCGGGTTCCGTGGTGCATACC
GGGGAAATCGGTGCAGGTAACGTCACCAAACTGGCAAATCAGGTCATTGTGGCGCTGAATATTGCCGCGATGTCAGAAGC
GTTAACGCTGGCAACTAAAGCGGGCGTTAACCCGGACCTGGTTTATCAGGCAATTCGCGGTGGACTGGCGGGCAGTACCG
TGCTGGATGCCAAAGCGCCGATGGTGATGGACCGCAACTTCAAGCCGGGCTTCCGTATTGATCTGCATATTAAGGATCTG
GCGAATGCGCTGGATACTTCTCACGGCGTCGGCGCACAACTGCCGCTCACAGCTGCGGTTATGGAGATGATGCAGGCACT
GCGTGCAGATGGTTTAGGAACGGCGGATCATAGCGCCCTGGCGTGCTACTACGAAAAACTGGCGAAAGTCGAAGTTACTC
GTTAA

Upstream 100 bases:

>100_bases
TCGGCAGCGATCTCGGCGTCTTCCGCTCTGCCACTCAGAAACTGGCTGATACCTTTAAAAAATAACCACCACCGCAACAA
GAGAGATGATTGATATGACT

Downstream 100 bases:

>100_bases
TGACGTCGTGCCCGGATGGCGTATTGCAATCCGGGCCTGGAATCCAGGCCGAATAAGAGAAACGCCCTCCGGCGTCATGT
GGTAACAGGCATAGGTATGA

Product: tartronate semialdehyde reductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGI
IEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHT
GEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR

Sequences:

>Translated_294_residues
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGI
IEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHT
GEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
>Mature_294_residues
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGI
IEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHT
GEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=35.4385964912281, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=29.4326241134752, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=100, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=43.9446366782007, Blast_Score=240, Evalue=7e-65,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=36.7857142857143, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1789092, Length=282, Percent_Identity=34.3971631205674, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=32.7272727272727, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24655230, Length=286, Percent_Identity=32.1678321678322, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922568, Length=286, Percent_Identity=32.1678321678322, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=25.531914893617, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GARR_ECOL6 (P0ABQ3)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755747.2
- ProteinModelPortal:   P0ABQ3
- SMR:   P0ABQ3
- EnsemblBacteria:   EBESCT00000045442
- GeneID:   1040122
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3880
- GeneTree:   EBGT00050000009837
- HOGENOM:   HBG729179
- OMA:   YSRILEN
- ProtClustDB:   PRK11559
- BRENDA:   1.1.1.60
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981
- PRINTS:   PR00076
- TIGRFAMs:   TIGR01505

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: =1.1.1.60

Molecular weight: Translated: 30428; Mature: 30428

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 170-170 BINDING 95-95 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVI
CEEEEEECCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCEE
ITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPV
EEECCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEECCCC
SGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALN
CCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHEEH
IAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHHHH
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVI
CEEEEEECCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCCEE
ITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPV
EEECCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEECCCC
SGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALN
CCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHEEH
IAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHHHH
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157