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The map label for this gene is ydfJ [H]

Identifier: 209399099

GI number: 209399099

Start: 2071499

End: 2072782

Strand: Reverse

Name: ydfJ [H]

Synonym: ECH74115_2159

Alternate gene names: 209399099

Gene position: 2072782-2071499 (Counterclockwise)

Preceding gene: 209400958

Following gene: 209399925

Centisome position: 37.2

GC content: 47.04

Gene sequence:

>1284_bases
ATGGATTTCCAGTTATATTCGCTCGGCGCAGCGTTAGTGTTTCATGAAATATTTTTTCCTGAATCATCAACGGCAATGGC
GTTAATTCTGGCAATGGGAACCTACGGTGCAGGTTATGTGGCGCGTATTGTCGGAGCATTTATTTTCGGCAAAATGGGCG
ACAGAATAGGGCGTAAAAAAGTGCTCTTTATTACCATCACCATGATGGGGATCTGTACCACCTTAATTGGAGTGTTACCG
ACCTATGCACAGATTGGTGTTTTTGCCCCCATCTTGCTGGTGACGCTGCGTATTATTCAGGGATTGGGTGCAGGTGCGGA
AATTTCCGGTGCCGGTACGATGCTGGCGGAATATGCGCCAAAAGGTAAGCGCGGAATTATCTCCTCATTTGTGGCTATGG
GAACTAACTGCGGAACCTTGAGCGCAACGGCAATCTGGGCCTTTATGTTCTTCATTCTCAGTAAAGAGGAACTGCTGGCG
TGGGGATGGCGTATACCGTTCCTGGCGAGCGTTGTCGTGATGGTCTTTGCTATCTGGTTGCGTATGAATCTGAAAGAAAG
CCCGGTCTTTGAGAAGGTTAACGACAGCAACCAACCGACAGCAAAACCTGCACCTGCTGGTAGCATATTCCAGAGCAAAT
CCTTCTGGCTGGCAACAGGGCTGCGTTTTGGTCAGGCAGGTAACTCCGGGTTAATTCAGACTTTCCTTGCAGGCTATTTA
GTACAGACGTTATTGTTTAACAAAGCAATTCCAACAGATGCATTGATGATCAGTTCGATTCCCGGCTTTATGACCATTCC
GTTCCTTGGTTGGTTATCCGATAAAATTGGTCGCCGGATCCCGTATATTATTATGAATACCTCCGCGATTGTGCTGGCAT
GGCCAATGCTTTCTATCATCGTAGATAAAAGCTATGCCCCGAGCACCATTATGGTTGCACTGATTGTGATTCATAACTGT
GCGGTGCTGGGATTATTTGCTCTGGAAAACATTACCATGGCAGAAATGTTCGGCTGTAAAAACCGCTTTACCCGGATGGC
TATTTCTAAAGAAATTGGTGGTCTTATCGCTTCCGGTTTTGGTCCTATCCTGGCGGGTATTTTCTGCACCATGACGGAAT
CCTGGTATCCGATCGCCATTATGATCATGGCATATTCAGTGATTGGTTTAATCTCTGCGCTGAAAATGCCAGAAGTGAAA
GACCGTGATTTAAGTGCGCTGGAAGACGCTGCGGAAGATCAACCGCGTGTTGTAAGAGCTGCGCAACCTTCCAGAAGTCT
GTAA

Upstream 100 bases:

>100_bases
GCAAAATCGAGATTAGGGATTTTTAGAAAAAATACAACCATCTGAAAAAACTTAGAAAACACCCAAAAACCCACAGAATA
GTAAAAAACATCTATGAATT

Downstream 100 bases:

>100_bases
ATCCTCAATCCCTTCTCTTACCGGAGAGGGGATTTTTATTCATATAACAAAACATGTAGCTTGCCATATTTAAATTTAAG
GAATTGTTATGGGAAATAAT

Product: inner membrane metabolite transport protein ydfJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLP
TYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNC
AVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVK
DRDLSALEDAAEDQPRVVRAAQPSRSL

Sequences:

>Translated_427_residues
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLP
TYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNC
AVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVK
DRDLSALEDAAEDQPRVVRAAQPSRSL
>Mature_427_residues
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLP
TYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNC
AVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVK
DRDLSALEDAAEDQPRVVRAAQPSRSL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=402, Percent_Identity=33.5820895522388, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=29.8823529411765, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=29.0865384615385, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788942, Length=369, Percent_Identity=31.9783197831978, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082231, Length=310, Percent_Identity=23.2258064516129, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46234; Mature: 46234

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
VLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAP
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCC
KGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
HCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHCCHHCCCCCCHHHHHHHHHHH
VQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSII
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
VDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEC
AQPSRSL
CCCCCCC
>Mature Secondary Structure
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKK
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
VLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAP
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCC
KGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RMNLKESPVFEKVNDSNQPTAKPAPAGSIFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
HCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHCCHHCCCCCCHHHHHHHHHHH
VQTLLFNKAIPTDALMISSIPGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSII
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
VDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEC
AQPSRSL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]