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The map label for this gene is mltF [H]

Identifier: 209399097

GI number: 209399097

Start: 3520301

End: 3521857

Strand: Direct

Name: mltF [H]

Synonym: ECH74115_3794

Alternate gene names: 209399097

Gene position: 3520301-3521857 (Clockwise)

Preceding gene: 209396380

Following gene: 209398568

Centisome position: 63.18

GC content: 50.67

Gene sequence:

>1557_bases
TTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCC
CTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCC
TGACGTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTA
AAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTCGATAATGGTAACGCCGACCTGCTGGCGGC
AGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTT
ATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTG
GTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGA
ATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTC
ACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACCTGGTTTAGCCCGTTAGATGGCGATAATACC
CTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGG
GCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCC
TGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAG
GCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCAAAAATACCGCGCAAAGCCTCGGCATTACGGATCGTAC
CGATGCCGAACAGAGCATCAGCGGTGGCGTGCGTTATTTGCAGGATATGATGAGTAAAGTGCCGGAAAGTGTGCCGGAGA
ACGAGCGGATCTGGTTTGCCCTCGCTGCGTACAATATGGGCTATGCGCATATGCAGGATGCCCGCGCCCTGACGGCAAAA
ACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCT
GACTTACGGCTACGCTCGTGGGCATGAAGCCTACGCTTATGTCGAAAATATTCGTAAGTATCAGATTAGCCTGGTGGGTT
ATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAG
TTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCT
GTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAA

Upstream 100 bases:

>100_bases
GGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACATTTTTTCAAGCTGGGACGCGCA
CGACACAGAGAATTAACTAA

Downstream 100 bases:

>100_bases
TCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCA
CTCATCCGCCAGTATTCCTT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQ
ATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQDARALTAK
TKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Sequences:

>Translated_518_residues
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQ
ATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQDARALTAK
TKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
>Mature_518_residues
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQ
ATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQDARALTAK
TKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=518, Percent_Identity=99.8069498069498, Blast_Score=1065, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 58318; Mature: 58318

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEIN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEEECCEEEECCC
GKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQ
CCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEEEHHEECHHHHCCCC
PGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVD
CCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHHHHHCCCCCCEEEEC
DKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCCH
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAE
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHH
QDMMSKVPESVPENERIWFALAAYNMGYAHMQDARALTAKTKGNPDSWADVKQRLPLLSQ
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHCC
KPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
CCCCCCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC
>Mature Secondary Structure
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEIN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEEECCEEEECCC
GKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQ
CCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEEEHHEECHHHHCCCC
PGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVD
CCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHHHHHCCCCCCEEEEC
DKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCCH
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAE
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHH
QDMMSKVPESVPENERIWFALAAYNMGYAHMQDARALTAKTKGNPDSWADVKQRLPLLSQ
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHCC
KPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
CCCCCCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA