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The map label for this gene is ecpD [H]

Identifier: 209398909

GI number: 209398909

Start: 1288803

End: 1289504

Strand: Reverse

Name: ecpD [H]

Synonym: ECH74115_1276

Alternate gene names: 209398909

Gene position: 1289504-1288803 (Counterclockwise)

Preceding gene: 209395888

Following gene: 209397811

Centisome position: 23.14

GC content: 46.15

Gene sequence:

>702_bases
ATGAACATTTTTGCATATTTACTGGTACTTGTATTTTCCATGAGCATGAGCAGCAGCGCGTTTGCCAGCGTGGTAATGAC
CGGAACCCGTATTATTTTCCCTGGTGACGCAAAGGAAAAAACCATCCAGTTGCGAAATACCAGCGATCAGCCCTATATCA
TTAATATCCATGTTGAGGATGAACGTGGTTCTGACAAGAATGTACCGTTTATGCCAACCCCGCAGACATTTCGCATGGAA
GCTGCCGCAGGTCAGGCGTTACGCCTGCTCTACACTGGTAATAATTTACCGCAGGATCGCGAGTCTGTTTTCTGGTTTAG
TTTCAGTCAACTACCTTATCTGAATAAGAATGATAAAAGTCAGAACCAGCTCATCCTGGCCCTGACTAATCGAGTCAAAA
TTTTCTATCGTCCCAGCTCGATTGTCGGTAAATCCAGTGACGCACCCAAAAACCTGACTTACCAGGTAAAACAGAACCGC
ATTGAAGTGACGAATCCCACGGGCTATTACGTCACAATTCGCGCCGCTGAACTGCTTAATAATGGTAAAAAAGTCCCCCT
CGCGAATTCGGTAATGATTGCTCCTCAAAGCACAACTGAATGGACACTACCCTCTGGCATCAGTGTCGCTCCCGGTGCGC
AGATCCATTTAGTGACCGTCAACGACTATGGCGTAAATGTTACGTCTGAGCATGCCTTATAA

Upstream 100 bases:

>100_bases
CTGAGCTACCTCTAATTAATCTACTGGCTGGCAGTAAATGCCAGCCAGTATTTCTTCGCACAATTAATAGTGATTTTTCA
TGGACATGGAAAAGATAAGA

Downstream 100 bases:

>100_bases
CCTCTTTCTCCTTTTTTGAGCGGTATCCTTTTTCACGCACGAGTACGTCGAGATGAATAAATCCGTTGTGTCAATTTCTG
CGGCAATGTTGGTTTTACTT

Product: gram-negative pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQLRNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRME
AAAGQALRLLYTGNNLPQDRESVFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLVTVNDYGVNVTSEHAL

Sequences:

>Translated_233_residues
MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQLRNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRME
AAAGQALRLLYTGNNLPQDRESVFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLVTVNDYGVNVTSEHAL
>Mature_233_residues
MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQLRNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRME
AAAGQALRLLYTGNNLPQDRESVFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLVTVNDYGVNVTSEHAL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=230, Percent_Identity=35.6521739130435, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1786743, Length=228, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789532, Length=230, Percent_Identity=30, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790771, Length=219, Percent_Identity=32.8767123287671, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788428, Length=224, Percent_Identity=29.9107142857143, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786936, Length=232, Percent_Identity=31.0344827586207, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787171, Length=220, Percent_Identity=29.0909090909091, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081806, Length=233, Percent_Identity=30.4721030042918, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788677, Length=201, Percent_Identity=30.3482587064677, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082203, Length=223, Percent_Identity=26.457399103139, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25894; Mature: 25894

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQLRNTSDQPYIINIHVED
CCHHHHHHHHHHHCCCCCHHHEEEEEECCEEEECCCCCCEEEEEECCCCCCEEEEEEEEC
ERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRESVFWFSFSQLPYLNKNDKS
CCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCC
QNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNRIEVTNPTGYYVTIRAAELLN
CCEEEEEEECCEEEEECCCHHCCCCCCCCCCEEEEEECCEEEEECCCCEEEEEEEHHHHC
NGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLVTVNDYGVNVTSEHAL
CCCCCCCCCEEEECCCCCCCEECCCCCEECCCCEEEEEEEECCCCEEECCCCC
>Mature Secondary Structure
MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQLRNTSDQPYIINIHVED
CCHHHHHHHHHHHCCCCCHHHEEEEEECCEEEECCCCCCEEEEEECCCCCCEEEEEEEEC
ERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRESVFWFSFSQLPYLNKNDKS
CCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCC
QNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNRIEVTNPTGYYVTIRAAELLN
CCEEEEEEECCEEEEECCCHHCCCCCCCCCCEEEEEECCEEEEECCCCEEEEEEEHHHHC
NGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLVTVNDYGVNVTSEHAL
CCCCCCCCCEEEECCCCCCCEECCCCCEECCCCEEEEEEEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]