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The map label for this gene is yfcG [H]

Identifier: 209398828

GI number: 209398828

Start: 3188153

End: 3188800

Strand: Direct

Name: yfcG [H]

Synonym: ECH74115_3442

Alternate gene names: 209398828

Gene position: 3188153-3188800 (Clockwise)

Preceding gene: 209399207

Following gene: 209399151

Centisome position: 57.22

GC content: 51.85

Gene sequence:

>648_bases
ATGATCGATCTCTATTTCGCCCCGACACCCAATGGCCACAAAATTACGCTGTTTCTCGAAGAAGCAGGGCTGGATTATCG
CTTGATAAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGCCCGGAATTTTTGCTCATTTCGCCTAACAACAAAATTCCGG
CAATTGTTGATCATTCTCCAGCCGATGGCGGCGAACCGCTAAGCCTCTTTGAATCTGGTGCCATTTTGTTGTATCTGGCT
GAGAAAACGGGGCTCTTTTTGAGTCATGAAACGCGTGAACGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGG
ACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATC
AGGTTGAAACTCAGCGTCTGTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGC
ATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATAGACCTCGCTATGTATCCGGCAGTCAA
GAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGG
ATAGTTAA

Upstream 100 bases:

>100_bases
CTATAGAGCCTTTTTGTCACTGTCACGTGACGAATACTCACGTAAGTCGCCGCGCTTTCTATAATTGTGGTGTTCAGATG
ATTAGACAACCGGAGCTCCA

Downstream 100 bases:

>100_bases
CAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATT
CGTATAAAGAACCTTCGTTT

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLLISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS

Sequences:

>Translated_215_residues
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLLISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS
>Mature_215_residues
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLLISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLA
EKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=215, Percent_Identity=99.0697674418605, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI87082195, Length=212, Percent_Identity=40.0943396226415, Blast_Score=151, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=34.3347639484979, Blast_Score=122, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28571670, Length=176, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864598, Length=205, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864592, Length=199, Percent_Identity=25.6281407035176, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864594, Length=193, Percent_Identity=25.3886010362694, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI85725204, Length=201, Percent_Identity=26.865671641791, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17737923, Length=201, Percent_Identity=26.865671641791, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45549270, Length=199, Percent_Identity=24.6231155778894, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646249, Length=202, Percent_Identity=27.2277227722772, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 24401; Mature: 24401

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLLISPNNKIPAIVDHSP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
ADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNH
CCCCCCHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEE
AMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS
EECHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFLLISPNNKIPAIVDHSP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
ADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFNH
CCCCCCHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEE
AMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS
EECHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]