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The map label for this gene is eutB [H]

Identifier: 209398786

GI number: 209398786

Start: 3384538

End: 3385899

Strand: Reverse

Name: eutB [H]

Synonym: ECH74115_3671

Alternate gene names: 209398786

Gene position: 3385899-3384538 (Counterclockwise)

Preceding gene: 209400026

Following gene: 209400550

Centisome position: 60.77

GC content: 55.58

Gene sequence:

>1362_bases
ATGAAACTAAAGACCACATTGTTCGGCAATGTATATCAGTTTAAGGATGTAAAAGAGGTGCTGGCTAAAGCCAACGAACT
GTGTTCGGGGGATGTGCTGGCGGGCGTCGCAGCGGCAAGCTCACAGGAGCGCGTGGCGGCAAAGCAGGTGTTGTCGGAAA
TGACCGTAGCGGACATCCGCAATAATCCGGTGATTGCCTATGAAGATGACTGCGTGACGCGGCTGATTCAGGACGATGTT
AACGAAACGGCCTACAACCAGATTAAAAACTGGAGCATCAGCGAACTGCGTGAGTATGTGCTGAGCGATGAAACCAGCGT
GGACGACATTGCCTTTACCCGCAAAGGGCTGACCTCGGAAGTGGTCGCGGCGGTAGCGAAGATTTGCTCCAACGCGGACC
TGATCTACGGCGCGAAGAAAATGCCGGTAATCAAAAAGGCCAATACCACCATCGGTATTCCGGGCACCTTCAGCGCCCGT
TTGCAGCCAAATGACACCCGTGACGACGTGCAAAGTATCGCCGCGCAAATCTACGAAGGGCTTTCCTTCGGGGTGGGCGA
TGCGGTGATCGGCGTTAACCCGGTGACTGACGACGTGGAAAACTTAAGCCGCGTGCTGGACACCATCTATGGCGTGATCG
ACAAATTCAACATCCCAACTCAGGGCTGCGTACTGGCGCACGTCACCACCCAGATCGAAGCGATTCGTCGCGGCGCGCCT
GGCGGACTGATTTTCCAGAGTATCTGTGGCAGCGAAAAAGGGCTGAAAGAGTTTGGCGTGGAACTGGCGATGCTCGACGA
AGCGCGCGCAGTGGGTGCAGAGTTCAACTGTATCGCCGGGGAAAACTGCCTCTACTTCGAAACCGGACAAGGTTCAGCGC
TATCCGCTGGCGCTAACTTCGGCGCAGACCAGGTGACGATGGAAGCACGTAACTATGGGCTGGCGCGTCATTACGATCCG
TTTATCGTCAACACCGTGGTCGGCTTTATTGGGCCGGAGTATCTCTACAACGACCGCCAGATTATCCGTGCGGGCTTAGA
AGATCACTTTATGGGCAAGCTGAGCGGCATCTCTATGGGCTGTGACTGCTGTTACACCAACCACGCTGACGCTGACCAGA
ACCTCAACGAAAACCTGATGATCCTGCTCGCCACCGCAGGCTGCAACTACATCATGGGGATGCCGCTGGGTGATGACATC
ATGCTCAACTACCAGACCACCGCATTCCACGATACCGCCACTGTGCGTCAGTTACTCAACCTGCGCCCGTCACCGGAGTT
TGAACGCTGGCTGGAAAGCATGGGCATTATGGCAAACGGTCGCCTGACCAAACGGGCGGGCGATCCGTCACTGTTCTTCT
GA

Upstream 100 bases:

>100_bases
GCGCGGGGGACTATATCGACATTGGTACGCCTCTTTTTGGCGGATCGGTTGTGCCGGTGACGGTGAAATCACTCGCATTT
CCTTCCTGAGGGAACGACTT

Downstream 100 bases:

>100_bases
TGACGCGGAGATAACACATCATGGATCAAAAACAGATTGAAGAAATTGTACGCAGCGTGATGGCGTCAATGGGACAAACG
GCCCCCGCGCCGTCAGAAGC

Product: ethanolamine ammonia-lyase, large subunit

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDV
NETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNCIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP
FIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

Sequences:

>Translated_453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDV
NETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNCIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP
FIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
>Mature_453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDV
NETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNCIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP
FIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=453, Percent_Identity=99.5584988962472, Blast_Score=930, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 49297; Mature: 49297

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
CCEEHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
NNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHH
VVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEG
HHHHHHHHHCCCCEEECCCCCCEEEECCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHH
LSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCC
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNCIAGENCLYFETGQGSALSAGANF
CCHHHHHHHCCCCCHHHHCCEEEEEHHHHHCCCCCEEEECCCEEEEECCCCCEEECCCCC
GADQVTMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CCHHEEHHHHHCCCHHCCCCHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHCCCCCC
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
CCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHC
LRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
CCCCCHHHHHHHHCCCEECCEEEECCCCCCCCC
>Mature Secondary Structure
MKLKTTLFGNVYQFKDVKEVLAKANELCSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
CCEEHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
NNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHH
VVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEG
HHHHHHHHHCCCCEEECCCCCCEEEECCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHH
LSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCC
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNCIAGENCLYFETGQGSALSAGANF
CCHHHHHHHCCCCCHHHHCCEEEEEHHHHHCCCCCEEEECCCEEEEECCCCCEEECCCCC
GADQVTMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CCHHEEHHHHHCCCHHCCCCHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHCCCCCC
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
CCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHC
LRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
CCCCCHHHHHHHHCCCEECCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]