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The map label for this gene is puuA [H]

Identifier: 209398672

GI number: 209398672

Start: 1834438

End: 1835856

Strand: Reverse

Name: puuA [H]

Synonym: ECH74115_1939

Alternate gene names: 209398672

Gene position: 1835856-1834438 (Counterclockwise)

Preceding gene: 209399768

Following gene: 209398619

Centisome position: 32.95

GC content: 52.92

Gene sequence:

>1419_bases
ATGGAAACCAATATCGTTGAAGTAGAGAACTTTGTTCAGCAGTCAGAAGAGAGGCGGGGTAGCGCCTTTACGCAGGAAGT
GAAACGCTACCTGGAGCGCTACCCGAATACGCAATATGTTGATGTTTTACTTACCGATTTGAATGGTTGCTTCCGTGGAA
AACGTATTCCTGTTTCAAGCCTGAAGAAGCTCGAGAAAGGGTGTTATTTCCCGGCCTCGGTATTTGCAATGGATATTCTG
GGCAACGTAGTAGAAGAAGCAGGTCTGGGTCAGGAAATGGGCGAGCCGGATCGTACCTGTGTTCCTGTTCTCGGTTCCTT
AACCCCTTCTGCCGCCGATCCAGAGTTTATCGGTCAGATGCTCCTGACCATGGTCGATGAAGATGGCGCTCCCTTTGACG
TTGAGCCGCGGAACGTTCTTAACCGCCTGTGGCAGCAGCTGCGCCAGCGCGGATTGTTCCCGGTCGTAGCGGTAGAGCTG
GAGTTCTATTTACTGGATCGCCAGCGCGACGCTGAAGGGTATCTGCAACCGCCCTGCGCGCCCGGCACTGATGACCGCAA
TACACAAAGCCAGGTTTACTCCGTTGATAACCTCAACCACTTCGCCGACGTGCTCAATGATATTGATGAACTGGCGCAGT
TACAGCTGATTCCGGCAGATGGCGCGGTCGCTGAGGCCTCGCCGGGTCAGTTTGAAATCAACCTTTACCATACTGATAAC
GTGCTGGAAGCCTGCGATGATGCGCTGGCACTAAAACGCCTGGTGCGTCTGATGGCAGAAAAGCATAAGATGCACGCCAC
TTTTATGGCGAAGCCGTATGAAGAGCACGCGGGCAGCGGAATGCATATCCATATCAGTATGCAAAATAATCGTGGCGAGA
ACGTGCTTTCTGACACAGAAGGCGAAGATTCGCCGCTGCTGAAAAAGATGCTCGCCGGGATGATTGACCTGATGCCGTCA
TCAATGGCGTTGCTGGCACCAAACGTGAACTCGTATCGCCGCTTCCAGCCGGGGATGTATGTACCGACGCAGGCGTCGTG
GGGCCATAATAACCGTACCGTCGCCCTGCGTATTCCATGCGGCGATCGTCATAATCACCGCGTGGAATATCGCGTGGCGG
GTGCCGATGCCAACCCATATCTGGTGATGGCAGCGATTTTTGCCGGTATTTTGCATGGCCTTGATAACGAGCTGCCGTTG
CAGGAAGAAGTCGAAGGTAACGGGCTGGAACAGGAAGGCTTACCCTTCCCGATTCGCCAGAGCGATGCCCTGGGTGAGTT
TATCGAGAATGATCACCTGCGCCGCTATTTAGGCGAACGGTTTTGCCATGTGTATCACGCCTGTAAAAATGATGAGCTGT
TGCAGTTTGAGCGCCTCATCACAGAAACAGAAATTGAGTGGATGTTGAAAAACGCATAG

Upstream 100 bases:

>100_bases
TAACATTTGACAAACATTTAGTTTGCATACAGATTCGAATGGTGGTCATTATATTTTACGCTTTGATAACGAGCGGAAAA
CAAACCAAAGGCGAAGAATC

Downstream 100 bases:

>100_bases
TACCCCTTGCGGGGTTGTTTCATCGGCCTGAGCGGAGCCAGGCCTGACATTTGCTGGTAATCCAGACATTTCCCGGATGT
CGCGTTATGGAGCGCAGGCA

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPS
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPL
QEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA

Sequences:

>Translated_472_residues
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPS
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPL
QEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
>Mature_472_residues
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPS
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPL
QEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=440, Percent_Identity=25.6818181818182, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=99.7881355932203, Blast_Score=978, Evalue=0.0,
Organism=Escherichia coli, GI1790301, Length=401, Percent_Identity=26.1845386533666, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 53208; Mature: 53208

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSS
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHH
LKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHH
LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEEECCC
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEEECCCCCCCCCCCC
GEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCC
SDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSS
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHH
LKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHH
LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEEECCC
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEEECCCCCCCCCCCC
GEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCC
SDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]