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The map label for this gene is yeiT

Identifier: 209398540

GI number: 209398540

Start: 3010389

End: 3011627

Strand: Direct

Name: yeiT

Synonym: ECH74115_3278

Alternate gene names: 209398540

Gene position: 3010389-3011627 (Clockwise)

Preceding gene: 209400436

Following gene: 209396337

Centisome position: 54.03

GC content: 51.41

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGCCGTTACTGGCGATTAAAGAAGCCTCTCGCTGTTT
ATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTC
GTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAG
AAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGA
TTTTGAACAACAAACCGGGATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTC
CTGCCGGATTGCAGGCCAGTGTGACGCTAACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGT
GGCTGGTTGCGTAACGGTATTCCGCAATTTCGGTTACCGCAATCAGTGCTGGATGCAGAAATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAATAACGAAATCGGTAAAACACTCACCCTGGAGCAACTGAAAGCGGAAAACCGCGCGGTAC
TGGTCACCGTGGGGTTATCAAGCGGTTCCGGGCTACCGCTGTTTGAGCATAGCGACGTTGAGATTGCCGTCGACTTCCTG
CTACGTGCACGACAGGCGCAAGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTCGCGATGGA
CGTAGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCGGTAACTTGCGTAGCGCGTGAAGAGTTAGATGAGTTTCCGGCAA
GCGAAAAAGAGTTTGCCAGCGCCCGGGAGCTGGGCGTTTCGATCATCGATGGATTCACGCCAGTAGCCGTGGAAGGCAAT
AAAGTCACGTTTAAGCATGTACGGTTATCGGGCGAACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCA
TGCCAGACTGGATGACTTTGCGAAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTATCAGACCCGCGATCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
CTTTCACAGTTTAAATTTCCCCACGCGCATAGCCTTAATATCAGCACGCAATATTTATTTAGCGTTCGCCTTAATTACTC
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGC
TATGAGATGTGTGCCAAAGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
LRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLSGELTMAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
LRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLSGELTMAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGG
WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFLL
RARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNK
VTFKHVRLSGELTMAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA

Homologues:

Organism=Homo sapiens, GI119943098, Length=440, Percent_Identity=32.2727272727273, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=48.8888888888889, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=98.3009708737864, Blast_Score=834, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=450, Percent_Identity=31.7777777777778, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=31.447963800905, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=29.1759465478842, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789243, Length=398, Percent_Identity=29.6482412060301, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=33.3333333333333, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=449, Percent_Identity=27.8396436525612, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=25.5965292841649, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=31.5668202764977, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=31.5668202764977, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=27.4774774774775, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=27.4774774774775, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=27.8026905829596, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=27.8026905829596, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEIT_ECO57 (Q8X645)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91008
- PIR:   H85852
- RefSeq:   NP_288729.1
- RefSeq:   NP_311065.1
- ProteinModelPortal:   Q8X645
- SMR:   Q8X645
- EnsemblBacteria:   EBESCT00000024249
- EnsemblBacteria:   EBESCT00000057174
- GeneID:   916742
- GeneID:   957019
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3401
- KEGG:   ecs:ECs3038
- GeneTree:   EBGT00050000009108
- HOGENOM:   HBG715033
- OMA:   VIDYGRC
- ProtClustDB:   PRK11749
- BioCyc:   ECOL83334:ECS3038-MONOMER
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- Gene3D:   G3DSA:1.10.1060.10
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; SSF46548 Helical_ferredxn

EC number: NA

Molecular weight: Translated: 44366; Mature: 44235

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
LRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDDFAKLEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEEECCCEEEHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHCCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
LRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDDFAKLEPQR
HHHHCHHHHCCCCCEEEECCEEEEEEEEECCCEEEHHCEEEEEECCCCCCCHHHHCCCCC
NTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
CCCCCCCCCCCCCEEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796