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The map label for this gene is cusS

Identifier: 209398281

GI number: 209398281

Start: 679257

End: 680705

Strand: Reverse

Name: cusS

Synonym: ECH74115_0650

Alternate gene names: 209398281

Gene position: 680705-679257 (Counterclockwise)

Preceding gene: 209398379

Following gene: 209397950

Centisome position: 12.22

GC content: 52.8

Gene sequence:

>1449_bases
ATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTGGCCACCATCGCGGC
GTTTTTCGCCTTTGCATGGATCATGATCCACTCAGTAAAAGTGCATTTTGCCGAGCAGGATATTAATGATTTAAAAGAGA
TTAGTGCCACCCTTGAACGCGTACTAAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTGGAAGATATCGTC
AGTGGTTATTCCAATGTGTTGATTTCCCTGGCAGATAGCCACGGTAAAACGGTGTATCACTCCCCCGGTGCGCCTGATAT
CCGCGAGTTTGCGCGCGATGCCATACCCGATAAAGACGCGCGGGGCGGCGAGGTGTTTCTCCTCTCCGGCCCGACGATGA
TGATGCCAGGCCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATCAGTTTGCCAGTTGGCCCACTGGTTGAC
GGCAAACCGATTTATACGCTCTACATCGCGCTTTCGATCGATTTTCATCTTCATTACATAAATGATTTGATGAATAAACT
TATTATGACCGCATCGGTCATCAGCATCCTGATCGTCTTTATCGTGCTATTGGCGGTTCATAAAGGTCACGCGCCGATCC
GCAGCGTCAGCCGTCAAATCCAGAATATCACCTCGAAAGATCTCGACGTCCGCCTTGACCCGCAGACCGTGCCGATTGAG
CTGGAACAGCTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACCCGCCAGTCCAATTTCTCAGC
GGATATCGCCCACGAAATTCGCACACCAATTACTAATCTCATAACGCAAACGGAAATCGCCCTCAGCCAGTCGCGCAGCC
AGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGCGATATGCTGTTT
CTCGCCCAGGCCGATAACAACCAGTTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGGCAAAGTGTTCGA
TTTTTTCGAGGCTTTAGCGGAAGATCGCGGCGTGGAGCTGCAGTTTGTTGGCGACGAGTGCCAGGTTGCGGGCGATCCGC
TGATGCTGCGTCGGGCGTTAAGCAACCTGCTTTCGAACGCCCTGCGTTATACGCCACCCGGAGAGGCTATTGTAGTGCGC
TGCCAGACGGTCGATCATCTGGTGCAAGTTATCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAGCACTTACCGCGATT
GTTTGACCGTTTCTACCGCGTTGACCCCTCCCGCCAACGAAAAGGCGAAGGTAGCGGCATTGGGCTGGCGATAGTGAAAT
CAATTGTTGTCGCGCATAAAGGCACGGTTGCGGTTACGTCAAATGCGCGGGGGACAAGGTTTGTGATTGTGTTGCCGGAG
AGGGGGTGA

Upstream 100 bases:

>100_bases
GATGTGGCGGTGAAGCGGCTACGCGGCAAAATCGACAACGACTTTGAGCCGAAGCTGATTCAAACCGTGCGCGGCGTGGG
TTACATGCTTGAGGTGCCGG

Downstream 100 bases:

>100_bases
TTATTAAGTTCAAATTCAGGGCGAGGGTTTACGGCGTTTTTCGCTAAATTTCCATCATTATAAACAAGCCACCTATCATA
TAAAGCAATGAATAATTAAA

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVR
CQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSNARGTRFVIVLPE
RG

Sequences:

>Translated_482_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVR
CQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSNARGTRFVIVLPE
RG
>Mature_482_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVR
CQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSNARGTRFVIVLPE
RG

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=97.7035490605428, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=299, Percent_Identity=33.4448160535117, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=34.3396226415094, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=30.6273062730627, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.5180722891566, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.5819935691318, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=31.2056737588652, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693157, Length=240, Percent_Identity=27.0833333333333, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790551, Length=284, Percent_Identity=28.5211267605634, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787374, Length=347, Percent_Identity=23.6311239193084, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.503355704698, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082128, Length=295, Percent_Identity=24.7457627118644, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=24.5762711864407, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=25.4098360655738, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CUSS_ECO57 (Q8XBY4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85555
- PIR:   H90704
- RefSeq:   NP_286295.1
- RefSeq:   NP_308635.1
- ProteinModelPortal:   Q8XBY4
- SMR:   Q8XBY4
- EnsemblBacteria:   EBESCT00000025289
- EnsemblBacteria:   EBESCT00000055578
- GeneID:   916966
- GeneID:   957566
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0708
- KEGG:   ecs:ECs0608
- GeneTree:   EBGT00050000008661
- HOGENOM:   HBG643531
- OMA:   TIMMPGH
- ProtClustDB:   PRK09835
- BioCyc:   ECOL83334:ECS0608-MONOMER
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00304
- SMART:   SM00387
- SMART:   SM00388
- TIGRFAMs:   TIGR01386

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 53910; Mature: 53910

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2588dcec)-; HASH(0x25aa9520)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEHHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVRCQTVDHLVQVIVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSNARGTRFVIVLPE
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
RG
CH
>Mature Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEHHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVRCQTVDHLVQVIVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSNARGTRFVIVLPE
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
RG
CH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796