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The map label for this gene is gadX [H]

Identifier: 209398111

GI number: 209398111

Start: 4510198

End: 4511022

Strand: Reverse

Name: gadX [H]

Synonym: ECH74115_4877

Alternate gene names: 209398111

Gene position: 4511022-4510198 (Counterclockwise)

Preceding gene: 209396216

Following gene: 209397355

Centisome position: 80.96

GC content: 40.73

Gene sequence:

>825_bases
ATGCAATCATTACATGGGAATTGTCTAATTGCGTACGCAAGACATAAATATATTCTCACCATGGTTAATGGTGAATATCG
CTATTTTAATGGCGGTGACCTGGTTTTTGCGGATGCAAGCCAAATTCGAGTAGATAAGTGTGTTGAAAATTTTGTATTAG
TATCAAGGGATACGCTTTCATTATTTCTCCCGATGCTCAAGGAGGAGGCATTAAATCTTCATGCACATAAAAAAGTTTCT
TCATTACTCGTTCATCACTGTAGCAGAGATATTCCTGTTTTTCAGGAAGTTGCGCAACTATCGCAGAATAAGAATCTTCG
CTATGCAGAAATGCTACGTAAAAGAGCATTAATCTTTGCGTTGTTATCTGTTTTTCTTGAGGATGATCACTTTATACCGC
TGCTTCTGAACGTTTTACAACCGAACATGCGAACACGAGTTTGTACGGTTATCAATAATAATATCGCTCATGAGTGGACA
CTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTACGCGAAGAAGAGACATCATATTC
ACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTCGCAG
TGTCCTGTGGATATCACAGCGTGTCGTATTTCATTTACGTCTTTCGAAATTATTATGGGATGACGCCCACAGAGTATCAG
GAGCGATCGGCGCAGGGATTGCCGAACCGTGACTCGGCGGCAAGTATTGTTGCGCAAGGGAATTTTTACGGCACTGACTG
TTCTGCGGAAGGAATAAGATTATAG

Upstream 100 bases:

>100_bases
CTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTT
TATGTAATATTAAGTCAACT

Downstream 100 bases:

>100_bases
AGTTTTACTCAGACATAAAAAAAACCCGGCATAGGGGACCGGGAAGAGGATAGTCTGCCGTCTCCAGACTAATAAACCGT
TATAACACTCCCTGTTGGCA

Product: DNA-binding transcriptional regulator GadX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL

Sequences:

>Translated_274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL
>Mature_274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL

Specific function: Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW. Negatively regulates perA expression in acidic conditions and posi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789933, Length=274, Percent_Identity=98.1751824817518, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1790557, Length=147, Percent_Identity=44.8979591836735, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786778, Length=194, Percent_Identity=39.6907216494845, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787776, Length=136, Percent_Identity=43.3823529411765, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786776, Length=132, Percent_Identity=39.3939393939394, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789932, Length=128, Percent_Identity=38.28125, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31373; Mature: 31373

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHEECCHHHH
LFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL
HHHHCCCCCCCCHHHEEECCCEECCCCCCCCCCC
>Mature Secondary Structure
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHEECCHHHH
LFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL
HHHHCCCCCCCCHHHEEECCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA