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The map label for this gene is maeB [H]

Identifier: 209398066

GI number: 209398066

Start: 3396339

End: 3398618

Strand: Reverse

Name: maeB [H]

Synonym: ECH74115_3684

Alternate gene names: 209398066

Gene position: 3398618-3396339 (Counterclockwise)

Preceding gene: 209400356

Following gene: 209397456

Centisome position: 60.99

GC content: 54.25

Gene sequence:

>2280_bases
ATGGATGACCAGTTAAAACAAAGTGCACTTGATTTCCATGAATTTCCAGTTCCAGGGAAAATCCAGGTTTCTCCAACCAA
GCCTCTGGCAACGCAGCGCGATCTGGCGCTGGCCTACTCACCAGGCGTTGCCGCACCTTGTCTTGAAATCGAAAAAGACC
CGTTAAAAGCCTACAAATATACCGCGCGCGGTAACCTGGTGGCGGTGATCTCTAACGGTACGGCGGTGCTGGGGTTAGGC
AACATTGGCGCGCTGGCAGGCAAACCGGTGATGGAAGGCAAGGGCGTTCTGTTTAAGAAATTCGCTGGGATTGATGTATT
TGACATTGAAGTTGATGAACTCGACCCGGACAAATTTATTGAAGTCGTCGCTGCGCTCGAACCGACCTTCGGCGGCATCA
ACCTCGAAGACATTAAAGCGCCAGAATGTTTCTATATTGAACAGAAACTGCGCGAGCGGATGAATATTCCGGTATTCCAC
GACGATCAGCACGGCACGGCAATTATCAGCACTGCCGCCATCCTCAACGGCTTGCGCGTGGTGGAGAAAAACATCTCTGA
CGTGCGTATGGTGGTTTCCGGCGCGGGTGCTGCAGCAATCGCCTGTATGAACCTGCTGGTAGCGTTGGGTCTGCAAAAAC
ATAACATCGTGGTTTGCGATTCAAAAGGCGTTATCTATCAGGGCCGTGAGCCAAACATGGCGGAAACCAAAGCCGCATAT
GCGGTAGTGGATGACGGCAAACGTACCCTCGATGATGTGATTGAAGGCGCAGATATTTTCCTCGGCTGTTCCGGCCCGAA
AGTGCTGACCCAGGAAATGGTGAAGAAAATGGCTCGAGCGCCAATGATCCTGGCGCTGGCGAACCCGGAACCGGAAATTC
TGCCGCCGCTGGCGAAAGAAGTGCGTCCGGATGCCATCATCTGTACCGGCCGTTCCGACTACCCGAACCAGGTGAACAAC
GTCCTTTGCTTCCCGTTCATCTTCCGTGGCGCGCTGGACGTTGGCGCAACCGCCATCAACGAAGAGATGAAACTGGCGGC
GGTACGCGCGATTGCAGAACTGGCCCATGCGGAACAGAGTGAAGTGGTGGCTTCAGCGTATGGCGATCAGGATCTGAGCT
TTGGCCCTGAATACATCATTCCAAAACCGTTTGATCCGCGTCTGATAGTCAAGATTGCTCCTGCGGTCGCGAAAGCGGCG
ATGGAGTCGGGTGTGGCGACGCGTCCGATTGCTGATTTCGACGTCTACATCGACAAGCTGACTGAGTTCGTTTACAAAAC
CAACCTGTTTATGAAGCCGATTTTCTCCCAGGCTCGCAAAGCGCCGAAGCGCGTAGTTCTGCCGGAAGGGGAAGAGGCGC
GCGTTCTGCATGCCACTCAGGAACTGGTAACGCTGGGACTGGCGAAACCGATCCTTATCGGTCGTCCGAACGTGATCGAA
ATGCGCATTCAGAAACTGGGCTTGCAGATCAAAGCGGGCGTTGATTTTGAGATCGTCAATAACGAATCCGATCCGCGCTT
TAAAGAGTACTGGACCGAATACTTCCAGATCATGAAGCGTCGCGGCGTCACTCAGGAACAGGCGCAGCGGGCGCTGATCA
GTAACCCGACAGTGATCGGCGCGATCATGGTTCAGCGTGGCGAAGCCGATGCAATGATTTGCGGTACGGTGGGTGATTAT
CATGAACATTTTAGCGTGGTGAAAAATGTCTTTGGTTATCGCGATGGCGTTCACACCGCAGGTGCAATGAACGCGCTGCT
GCTGCCGAGTGGTAACACCTTTATTGCCGATACCTACGTCAATGACGAACCAGACGCAGAAGAGCTGGCGGAGATCACCT
TGATGGCGGCAGAAACTGTCCGTCGTTTCGGTATTGAACCGCGCGTTGCTCTGTTGTCGCACTCCAACTTTGGTTCTTCT
GACTGCCCGTCGTCGAGCAAAATGCGTCAGGCGCTGGAACTGGTCAGGGAACGTGCGCCAGAACTGATGATTGATGGTGA
AATGCACGGCGATGCAGCGCTGGTGGAAGCGATTCGCAACGACCGTATGCCGGACAGCCCTTTGAAAGGTTCTGCCAATA
TTCTGGTGATGCCGAACATGGAAGCTGCCCGCATTAGTTACAACTTACTGCGCGTTTCCAGCTCGGAAGGTGTGACTGTC
GGTCCGGTGCTGATGGGCGTGGCGAAACCGGTTCACGTGTTAACGCCGATCGCATCGGTGCGTCGTATCGTCAACATGGT
GGCGCTGGCCGTGGTTGAAGCGCAAACCCAACCGCTGTAA

Upstream 100 bases:

>100_bases
GTCTGTAGACTCCGGCAAGGTAATAATGTGCGCCACGTTGTGGGCAGGGGCTGTTGCCCACACACTTTATTTGTGAACGT
TACGTGAAAGGAACAACCAA

Downstream 100 bases:

>100_bases
TTCTTTTTTAACTCTCACGCTTACCCTGAATATTCAGGGTAAGCGGTTTAGCTGCAATATATTAGTAAAGCTTATTACTG
AGTTTGCGAATAATAAAAAA

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDY
HEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
DCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL

Sequences:

>Translated_759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDY
HEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
DCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
>Mature_759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDY
HEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
DCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=402, Percent_Identity=25.8706467661692, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI62420882, Length=345, Percent_Identity=26.3768115942029, Blast_Score=77, Evalue=8e-14,
Organism=Homo sapiens, GI62420880, Length=345, Percent_Identity=26.3768115942029, Blast_Score=77, Evalue=8e-14,
Organism=Homo sapiens, GI239049447, Length=345, Percent_Identity=26.3768115942029, Blast_Score=77, Evalue=8e-14,
Organism=Escherichia coli, GI1788806, Length=759, Percent_Identity=99.8682476943347, Blast_Score=1546, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=349, Percent_Identity=31.5186246418338, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1788800, Length=326, Percent_Identity=34.6625766871166, Blast_Score=167, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537199, Length=398, Percent_Identity=25.8793969849246, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=268, Percent_Identity=29.8507462686567, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24646388, Length=346, Percent_Identity=28.0346820809249, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24646386, Length=346, Percent_Identity=28.0346820809249, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281362672, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281362674, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356279, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 82428; Mature: 82428

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKY
CCCHHHHHHCCHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCHHCCCCCHHHEEE
TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
EVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRV
HHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHEEE
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE
EEECCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHH
EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVATRPIADFDVYIDKL
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHH
TEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
HHHHHHHCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHH
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIG
HHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
AIMVQRGEADAMICGTVGDYHEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYV
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECC
NDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ELMIDGEMHGDAALVEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
CEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEEECCCCCEE
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKY
CCCHHHHHHCCHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCHHCCCCCHHHEEE
TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
EVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRV
HHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHEEE
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE
EEECCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHH
EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVATRPIADFDVYIDKL
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHH
TEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
HHHHHHHCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHH
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIG
HHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
AIMVQRGEADAMICGTVGDYHEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYV
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECC
NDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ELMIDGEMHGDAALVEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
CEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEEECCCCCEE
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]