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The map label for this gene is mhpA [H]

Identifier: 209398053

GI number: 209398053

Start: 430810

End: 432474

Strand: Direct

Name: mhpA [H]

Synonym: ECH74115_0422

Alternate gene names: 209398053

Gene position: 430810-432474 (Clockwise)

Preceding gene: 209396585

Following gene: 209399057

Centisome position: 7.73

GC content: 56.94

Gene sequence:

>1665_bases
ATGGCAATACAACACCCTGACATCCAGCCTGCTGTTAACCATAGCGTTCAGGTGGCGATCGCTGGTGCCGGTCCGGTCGG
GCTGATGATGGCGAACTATCTCGGCCAGATGGGCATTGACGTGCTGGTGGTGGAGAAACTCGATAAGTTGATCGACTACC
CGCGTGCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATAATGTTCTGCCGCATACT
ACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAGGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGG
CTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGC
GCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGG
CTGCGGGAAATAGTCAAAGCCCAGTGGCTGGTGGCCTGTGATGGTGGGGCAAGTTTTGTCCGTCGCACCCTGAATGTGCC
GTTTGAAGGTAAAACTGCGCCAAATCAGTGGATTGTGGTAGATATCGCCAACGATCCGTTAAGTACGCCGCATATCTATT
TGTGTTGCGATCCGGTGCGCCCGTATGTTTCTGCCGCGCTACCTCATGCGGTACGTCGCTTTGAATTTATGGTGATGCCG
GGAGAAACCGAAGAACAGCTGCGTGAGCCGCAAAATATGCGCAAGCTGTTAAGCAAAGTGCTGCCTAATCCGGACAATGT
TGAATTGATTCGCCAGCGTGTCTACACCCACAACGCGCGACTGGCGCAACGTTTCCGTATTGATCGCGTACTGCTGGCGG
GCGATGCCGCGCACATCATGCCGGTATGGCAGGGGCAGGGCTATAACAGTGGTATGCGCGACGCCTTTAACCTCGCATGG
AAACTGGCGTTGGTTATCCAGGGGAAAGCCCGCGATGCGCTGCTCGATACCTATCAACAAGAACGTCGCGATCACGCCAA
AGCGATGATTGACCTGTCCGTGACGGCGGGCAACGTGCTGGCTCCGCCGAAACGCTGGCAGGGTACGTTACGTGACGGCG
TTTCCTGGCTGCTGAATTATCTGCCGCCAGTAAAACGCTACTTCCTCGAAATGCGCTTCAAGCCGATGCCGCAATATTAC
GGCGGTGCGCTGGTGCGTGAGGGCGAAGCGAAGCACTCTCCGGTCGGCAAGATGTTTATTCAGCCGAAAGTCACGCTGGA
AAACGGCGACGTGACGCTGCTCGATAACGCGATCGGCGCGAACTTCGCGGTAATTGGCTGGGGATGCAATCCACTGTGGG
GGATGAGCGACGAGCAAATCCAGCAGTGGCGCGCGTTGAGCACACGCTTCATTCAGGTGGTGCCGGAAGTGCAAATTCAT
ACCGCACAGGATAACCACGACGGCGTACTACGCGTGGGGGATACGCAAGGTCGCCTGCGTAGCTGGTTCGCGCAACACAA
TGCTTCGCTGGTGGTGATGCGCCCGGATCGCTTTGTTGCCGCCACCGCCATTCCGCAAACACTGGGCAAGACCCTGAATA
AACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTCTGTCGAAAAGGTAGCCTGA

Upstream 100 bases:

>100_bases
ACTGAGCGCACAATAAAAAATCATTTACATATTTTTAACAAAATAAGTTGCGCTGTACTGTGCGCGCAACGGCATTTTGT
CCGAGTCGTGAGGTACTGAA

Downstream 100 bases:

>100_bases
TATGCACGCTTATCTTCATTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCACAAGAGGTACTCGATGAGG
TCAATGGCGTGATTGCCAGC

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: NA

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHT
TPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAW
KLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYY
GGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALSTRFIQVVPEVQIH
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA

Sequences:

>Translated_554_residues
MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHT
TPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAW
KLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYY
GGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALSTRFIQVVPEVQIH
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA
>Mature_553_residues
AIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTT
PWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEGL
REIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMPG
ETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWK
LALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYG
GALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALSTRFIQVVPEVQIHT
AQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=554, Percent_Identity=99.2779783393502, Blast_Score=1138, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 62169; Mature: 62037

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGID
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
DEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVD
HHHHHHHHHHCHHHHHCCCCCCHHHHEEECCCCCEEEECCCCHHHCCCCCCCCEECCCHH
AVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEGLREIVKAQWLVACDGGASFV
HHHHHHHHHCCCEEEEHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCEEEEECCCHHHH
RRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEECC
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIM
CCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHEE
PVWQGQGYNSGMRDAFNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVL
EEECCCCCCCCHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
APPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYGGALVREGEAKHSPVGKMFI
CCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCCEEEE
QPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALSTRFIQVVPEVQIH
CCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMT
ECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
LTRPDADVSVEKVA
CCCCCCCCCHHCCC
>Mature Secondary Structure 
AIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGID
CCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
DEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVD
HHHHHHHHHHCHHHHHCCCCCCHHHHEEECCCCCEEEECCCCHHHCCCCCCCCEECCCHH
AVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEGLREIVKAQWLVACDGGASFV
HHHHHHHHHCCCEEEEHHHHHHHHCCCCCCEEEEEECHHHHHHHHHCCEEEEECCCHHHH
RRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEECC
GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIM
CCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHEE
PVWQGQGYNSGMRDAFNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVL
EEECCCCCCCCHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
APPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYGGALVREGEAKHSPVGKMFI
CCCHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCCEEEE
QPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALSTRFIQVVPEVQIH
CCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMT
ECCCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHH
LTRPDADVSVEKVA
CCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA