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The map label for this gene is dacD [H]

Identifier: 209397891

GI number: 209397891

Start: 2661511

End: 2662683

Strand: Reverse

Name: dacD [H]

Synonym: ECH74115_2866

Alternate gene names: 209397891

Gene position: 2662683-2661511 (Counterclockwise)

Preceding gene: 209400917

Following gene: 209395889

Centisome position: 47.79

GC content: 49.28

Gene sequence:

>1173_bases
TTGCTGTTGAAACGCCGTCTTATTATTGCTGCTTCTTTGTTCATTTTTAACTTATCGTCTGGTTTTGCGGCGGAAAACAT
TCCTTTTTCACCTCAGCCTCCAGAGATTCATGCCGGCTCCTGGGTATTGATGGATTACACCACCGGTCAGATCCTCACCG
CGGGTAATGAGCATCAACAGCGCAATCCCGCCAGCCTGACAAAGCTGATGACGGGCTATGTCGTGGATCGCGCTATCGAT
AGTCATCGCATTACGCCAGACGATATTGTCACCGTGGGGCGCGATGCGTGGGCGAAAGATAATCCAGTGTTTGTCGGTTC
TTCACTGATGTTTTTGAAAGAGGGCGATCGCGTATCGGTACGTGATTTAAGCCGTGGTTTAATTGTGGATTCCGGAAATG
ACGCTTGTGTTGCACTGGCTGACTATATTGCCGGTGGGCAACGGCAGTTTGTTGAAATGATGAACAACTATGCCGAGAAG
CTGCATCTCAAGGATACGCATTTTGAAACAGTGCATGGTCTGGATGCACCAGGTCAGCATAGCTCGGCTTATGATTTAGC
TGTGCTTTCTCGCGCTATCATCCACGGCGAGCCCGAGTTTTATCATATGTACAGTGAGAAAAGTCTCACCTGGAACGGTA
TCACCCAGCAAAACCGTAACGGGTTATTGTGGGATAAAACCATGAATGTTGACGGCCTGAAAACGGGTCATACTTCTGGT
GCCGGGTTTAATCTCATTGCTTCGGCTGTAGATGGGCAGCGTCGTCTCATTGCAGTGGTAATGGGGGCTGACAGTGCAAA
AGGTCGTGAGGAAGAGGCAAGAAAATTACTGCGTTGGGGGCAACAAAACTTTACTACGGTGCAAATTTTGCACCGTGGGA
AAAAGGTCGGTACGGAACGCATCTGGTATGGCGATAAAGAAAATATCGCCCTGGGAACGGAACAAGAGTTCTGGATGGTG
CTACCGAAAGCCGAAATTCCACATATCAAAGCCAAATATACCCTTGATGGTAAAGAGCTCACCGCGCCAATTAGCGCCCA
TCAGCGGGTAGGGGAAATTGAACTTTACGACCGTGATAAACAGGTGGCGCACTGGCCGCTGGTTACCCTGGAATCTGTCG
GGGAAGGCAGCATGTTTTCTCGCCTGAGTGATTATTTCCACCATAAGGCCTGA

Upstream 100 bases:

>100_bases
CAGATAAAAATGAGAGGGTAGTCACATTTTCTTGCACTTTATTCCAGCCAGTTCATAAGTATTTCCGTAAAAAGAACAGC
TATTTGAAACTCCTGAGGGT

Downstream 100 bases:

>100_bases
CCTTTCTTTTGCAGCAGACTGGCAGGAGTGCGAGTCTGCTCGCATAATCAACACTCATTCCTTGTGGTTTTAATATTGCA
ACTATACTGTATATAAAAAC

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLLKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDYTTGQILTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRITPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEK
LHLKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLRWGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMV
LPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA

Sequences:

>Translated_390_residues
MLLKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDYTTGQILTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRITPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEK
LHLKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLRWGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMV
LPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA
>Mature_390_residues
MLLKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDYTTGQILTAGNEHQQRNPASLTKLMTGYVVDRAID
SHRITPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEK
LHLKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSG
AGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLRWGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMV
LPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=388, Percent_Identity=99.2268041237113, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1786851, Length=392, Percent_Identity=47.4489795918367, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787062, Length=385, Percent_Identity=47.7922077922078, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI87082054, Length=285, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43543; Mature: 43543

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDYTTGQILTAGNEHQQ
CCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
RNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCEEECCEEEEEECCCEEEH
RDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLHLKDTHFETVHGLDAPGQH
HHCCCCEEEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCCC
SSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSG
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCCC
AGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLRWGQQNFTTVQILHRGKKVGTER
CCHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHCCCCE
IWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDK
EEECCCCCEEECCCCCEEEEECCCCCCCEEEEEEECCHHEECCHHHHHCCCCEEEECCCC
QVAHWPLVTLESVGEGSMFSRLSDYFHHKA
CEEECCEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDYTTGQILTAGNEHQQ
CCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
RNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVFVGSSLMFLKEGDRVSV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCEEECCEEEEEECCCEEEH
RDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLHLKDTHFETVHGLDAPGQH
HHCCCCEEEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCCC
SSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSG
CCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCCC
AGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLRWGQQNFTTVQILHRGKKVGTER
CCHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHCCCCE
IWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLDGKELTAPISAHQRVGEIELYDRDK
EEECCCCCEEECCCCCEEEEECCCCCCCEEEEEEECCHHEECCHHHHHCCCCEEEECCCC
QVAHWPLVTLESVGEGSMFSRLSDYFHHKA
CEEECCEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]