Click here to switch to the map view.

The map label for this gene is yeaI [H]

Identifier: 209397879

GI number: 209397879

Start: 2378425

End: 2379759

Strand: Direct

Name: yeaI [H]

Synonym: ECH74115_2509

Alternate gene names: 209397879

Gene position: 2378425-2379759 (Clockwise)

Preceding gene: 209397249

Following gene: 209395799

Centisome position: 42.68

GC content: 38.88

Gene sequence:

>1335_bases
ATGGGTACACAAAAATTAAAAGCTCAAAGCTTTTTTATTTTCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTAC
CTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTA
ACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCA
TTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGAC
AAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTT
ATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACTGGCATTCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTT
CCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAATCATAC
TGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGTACAC
GATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTG
GATGAGTATAATTATACCATCTGGTATATCAGTCGCGGGATTGAAGTCTCCAGTAAACTGTTTGTTGTGTCTTTTCTGAT
TTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTGGCAGTTCATGATGTGCTGACCAATATTTATAATCGGCGCT
ACTTTTTCAACAGCGTAGAGTCATTATTGTCGCGACCTGTTGTTAAGGACTTCTGTGTCATGCTGGTTGATATTAATCAG
TTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTTTCAATTGTCGATATTATCCAGCAAAG
CATCCACCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGG
CAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAA
ATGACCATCAGTATTGGCACAGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGC
CTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAA

Upstream 100 bases:

>100_bases
ATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTG
CTCTCCTTTTTATGGTTTCT

Downstream 100 bases:

>100_bases
GTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCC
CAGCGGCGCAGAGATGAGTA

Product: diguanylate cyclase (GGDEF) domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLA
FLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVF
PLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
FKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
MTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI

Sequences:

>Translated_444_residues
MGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLA
FLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVF
PLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
FKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
MTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI
>Mature_443_residues
GTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFP
LLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLD
EYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQF
KRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQM
TISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1788085, Length=444, Percent_Identity=99.7747747747748, Blast_Score=900, Evalue=0.0,
Organism=Escherichia coli, GI1787262, Length=449, Percent_Identity=37.1937639198218, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=30.939226519337, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082007, Length=227, Percent_Identity=29.5154185022026, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786584, Length=234, Percent_Identity=26.4957264957265, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=27.810650887574, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=28.7292817679558, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 50775; Mature: 50644

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
MLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHH
LSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVD
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
ECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVE
HCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGE
HHHHCCHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCH
VFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLV
HHHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEE
MTEADKALREAKSEGGNKVIIHHI
EEHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
GTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
MLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHH
LSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVD
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
ECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVE
HCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIHPDDILARLEGE
HHHHCCHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCH
VFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLV
HHHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEE
MTEADKALREAKSEGGNKVIIHHI
EEHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]