Click here to switch to the map view.

The map label for this gene is xdhB

Identifier: 209397706

GI number: 209397706

Start: 3845342

End: 3846220

Strand: Direct

Name: xdhB

Synonym: ECH74115_4157

Alternate gene names: 209397706

Gene position: 3845342-3846220 (Clockwise)

Preceding gene: 209396943

Following gene: 209400478

Centisome position: 69.01

GC content: 53.36

Gene sequence:

>879_bases
ATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGGCTGACAACCCGCAGGCCAA
ACTGCTCGCCGGTGGCACTGACGTACTGATTCAGCTCCACCATCACAATGACCGTTATCGCCATATTGTTGATATTCATA
ATCTGGCGGAGCTGCGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTA
ATAGAAGATTCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCTCGTCCATTGCTGGACCGCAGATCCGTAA
CGTCGCTACCTACGGTGGAAATATTTGCAACGGTGCCACCAGCGCAGATTCTGCCACGCCAACGCTCATTTATGACGCGA
AACTGGAGATCCACTCCCCACGCGGTGTTCGTTTCGTCCCGATTAATGGCTTTCACACCGGGCCGGGCAAAGTGTCTCTT
GAGCATGACGAAATCCTTGTCGCCTTTCATTTTCCGCCCCAGCCGAAAGAACACGTGGGCAGCGCGCATTTTAAATATGC
CATGCGCGACGCAATGGATATTTCAACGATTGGCTGTGCCGCACATTGCCGACTGGATAACGGCAATTTCAGCGAATTAC
GCCTGGCATTTGGTGTTGCCGCGCCAACGCCGATTCGCTGCCAACATGCCGAACAGACTGCACAAAATGCGCCATTAAAC
CTGCAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTT
TCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGAAAATTGCAATGA

Upstream 100 bases:

>100_bases
GTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGG
ATTGATTTGAGGATAACATC

Downstream 100 bases:

>100_bases
ATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCATGCCGTACCAGGCACGCCGCTC
TCGGAATTACTCCGCGAACA

Product: xanthine dehydrogenase subunit XdhB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHNDRYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQL
IEDSITQRHLPALCAAASSIAGPQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFGVAAPTPIRCQHAEQTAQNAPLN
LQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ

Sequences:

>Translated_292_residues
MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHNDRYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQL
IEDSITQRHLPALCAAASSIAGPQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFGVAAPTPIRCQHAEQTAQNAPLN
LQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ
>Mature_292_residues
MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHNDRYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQL
IEDSITQRHLPALCAAASSIAGPQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFGVAAPTPIRCQHAEQTAQNAPLN
LQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ

Specific function: Presumed to be a dehydrogenase, but possibly an oxidase. Participates in limited purine salvage (requires aspartate) but does not support aerobic growth on purines as the sole carbon source (purine catabolism)

COG id: COG1319

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1789231, Length=292, Percent_Identity=98.972602739726, Blast_Score=597, Evalue=1e-172,
Organism=Drosophila melanogaster, GI17737937, Length=274, Percent_Identity=26.6423357664234, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XDHB_ECO57 (Q8X6C5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91096
- PIR:   H85941
- RefSeq:   NP_289437.2
- RefSeq:   NP_311767.1
- ProteinModelPortal:   Q8X6C5
- SMR:   Q8X6C5
- EnsemblBacteria:   EBESCT00000025440
- EnsemblBacteria:   EBESCT00000056634
- GeneID:   916440
- GeneID:   958358
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4206
- KEGG:   ecs:ECs3740
- GeneTree:   EBGT00050000011137
- HOGENOM:   HBG695787
- OMA:   NICRASP
- ProtClustDB:   PRK09971
- BioCyc:   ECOL83334:ECS3740-MONOMER
- InterPro:   IPR005107
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR002346
- Gene3D:   G3DSA:3.30.390.50
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10

Pfam domain/function: PF03450 CO_deh_flav_C; PF00941 FAD_binding_5; SSF55447 CO_deh_flav_C; SSF56176 FAD-binding_2

EC number: =1.17.1.4

Molecular weight: Translated: 31561; Mature: 31561

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS51387 FAD_PCMH

Important sites: BINDING 165-165 BINDING 184-184

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHNDRYRHIVDIHNLAELRG
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCCHHEEHHHHHHHHHCC
ITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAGPQIRNVATYGGNICNGAT
CEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCC
SADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSLEHDEILVAFHFPPQPKEHVG
CCCCCCCEEEEEEEEEEECCCCEEEEEECCEECCCCCEEEECCCEEEEEECCCCCHHHCC
SAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFGVAAPTPIRCQHAEQTAQNAPLN
CHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHEEEEEECCCCCCCEECCHHHHHHCCCCC
LQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHNDRYRHIVDIHNLAELRG
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEECCCCCHHEEHHHHHHHHHCC
ITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAGPQIRNVATYGGNICNGAT
CEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCC
SADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSLEHDEILVAFHFPPQPKEHVG
CCCCCCCEEEEEEEEEEECCCCEEEEEECCEECCCCCEEEECCCEEEEEECCCCCHHHCC
SAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFGVAAPTPIRCQHAEQTAQNAPLN
CHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHEEEEEECCCCCCCEECCHHHHHHCCCCC
LQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMTKKVISEAVAAAGGKLQ
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796