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The map label for this gene is dkgB

Identifier: 209397612

GI number: 209397612

Start: 232479

End: 233282

Strand: Direct

Name: dkgB

Synonym: ECH74115_0220

Alternate gene names: 209397612

Gene position: 232479-233282 (Clockwise)

Preceding gene: 209398642

Following gene: 209396743

Centisome position: 4.17

GC content: 46.39

Gene sequence:

>804_bases
ATGGCTATCCCTGCATTTGGTTTAGGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGAAAACGGCGCTTGA
ACTTGGTTATCGCGCAATTGATACTGCACAAATCTATGATAACGAAGCCGCAGTAGGTCTGGCGATTGCAGAAAGTGGCG
TGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCGAGTCTAAAA
GAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGT
TGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAAGAAGGGTTGACGCGTGAGATCGGTATTTCCAACTTCACGATCC
CATTGATGGAAAAGGCGATTGCTGCTGTTGGCGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAA
AACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCT
GAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGG
AAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTGAAAACCTGGAGAGTAATCTTAAGGCACAAAATTTACAGCTTGAT
GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAAGGTCTGGCTCCTGAATGGGA
TTAA

Upstream 100 bases:

>100_bases
AAAATCGCAAAAATCCTCTGCGTTTTACGCCCTTTTTCCGCAACATTCTAAAGCCCATAATCACCTCAGTTAACGAAAAT
AGCATTAAAAGAGGCATATT

Downstream 100 bases:

>100_bases
GCCTCTCTGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTAC
CGCACGGTCGCTGTAATAGA

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLK
ESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD
AEDKKAIAALDCNDRLVSPEGLAPEWD

Sequences:

>Translated_267_residues
MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLK
ESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD
AEDKKAIAALDCNDRLVSPEGLAPEWD
>Mature_266_residues
AIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKE
SLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQN
RKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLDA
EDKKAIAALDCNDRLVSPEGLAPEWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG)

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family

Homologues:

Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=32.3636363636364, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=30.8219178082192, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=30.8219178082192, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=30.8219178082192, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=30.4794520547945, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.8969072164948, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=30.976430976431, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=30.1038062283737, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=30.1038062283737, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=31.3993174061433, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=34.0277777777778, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=30.8771929824561, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=29.7658862876254, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI300116273, Length=269, Percent_Identity=30.8550185873606, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=29.6296296296296, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.9352517985612, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=38.5964912280702, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=38.5964912280702, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=98.501872659176, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=39.0625, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=28.6245353159851, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI48994888, Length=271, Percent_Identity=29.520295202952, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787674, Length=257, Percent_Identity=26.8482490272374, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=35.7933579335793, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=35.8620689655172, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.7638888888889, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71998625, Length=264, Percent_Identity=31.4393939393939, Blast_Score=140, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=267, Percent_Identity=31.8352059925094, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17561298, Length=276, Percent_Identity=31.1594202898551, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=277, Percent_Identity=31.7689530685921, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=281, Percent_Identity=31.3167259786477, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17566692, Length=262, Percent_Identity=32.0610687022901, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.8707482993197, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=27.3972602739726, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=29.368029739777, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324694, Length=276, Percent_Identity=35.8695652173913, Blast_Score=158, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=36.5248226950355, Blast_Score=155, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=30.0653594771242, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=27.027027027027, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319625, Length=275, Percent_Identity=30.1818181818182, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320079, Length=271, Percent_Identity=29.1512915129151, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24662789, Length=290, Percent_Identity=31.3793103448276, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=32.6530612244898, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=31.3588850174216, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI221378297, Length=314, Percent_Identity=30.8917197452229, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=31.1188811188811, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=31.1418685121107, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=34.6020761245675, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.8245614035088, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=33.9100346020761, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=28.5211267605634, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DKGB_ECO57 (Q8X7Z7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- EMBL:   AB035926
- PIR:   C85505
- PIR:   C90654
- RefSeq:   NP_285895.1
- RefSeq:   NP_308230.1
- ProteinModelPortal:   Q8X7Z7
- SMR:   Q8X7Z7
- EnsemblBacteria:   EBESCT00000029123
- EnsemblBacteria:   EBESCT00000056702
- GeneID:   914031
- GeneID:   956971
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0229
- KEGG:   ecs:ECs0203
- GeneTree:   EBGT00050000009262
- HOGENOM:   HBG605727
- OMA:   LAWAMQL
- ProtClustDB:   PRK11172
- BioCyc:   ECOL83334:ECS0203-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210
- Gene3D:   G3DSA:3.20.20.100
- PANTHER:   PTHR11732
- PIRSF:   PIRSF000097
- PRINTS:   PR00069

Pfam domain/function: PF00248 Aldo_ket_red; SSF51430 Aldo/ket_red

EC number: =1.1.1.274

Molecular weight: Translated: 29439; Mature: 29308

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1; PS00062 ALDOKETO_REDUCTASE_2; PS00063 ALDOKETO_REDUCTASE_3

Important sites: ACT_SITE 39-39 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGLAIAESGVPRHELY
CCCCCCCCCEEEECCHHHHHHHHHHHHHCHHHCCHHHEECCCCEEEEEEECCCCCCEEEE
ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKE
EEEEHEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT
CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEEHHH
LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCCHHHHHHHCCCCCCEEEC
AEDKKAIAALDCNDRLVSPEGLAPEWD
CCCCCEEEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure 
AIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGLAIAESGVPRHELY
CCCCCCCCEEEECCHHHHHHHHHHHHHCHHHCCHHHEECCCCEEEEEEECCCCCCEEEE
ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKE
EEEEHEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC
GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT
CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEEHHH
LAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCCHHHHHHHCCCCCCEEEC
AEDKKAIAALDCNDRLVSPEGLAPEWD
CCCCCEEEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008