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The map label for this gene is tmcA [H]

Identifier: 209397566

GI number: 209397566

Start: 3414056

End: 3416071

Strand: Reverse

Name: tmcA [H]

Synonym: ECH74115_3696

Alternate gene names: 209397566

Gene position: 3416071-3414056 (Counterclockwise)

Preceding gene: 209398291

Following gene: 209399161

Centisome position: 61.31

GC content: 55.01

Gene sequence:

>2016_bases
ATGGCTGAACTGACTGCGCTTCACACATTAACAGCGCAAATGAAACGTGAAGGGATCCGCCGCTTGCTGGTGTTGAGCGG
GGAAGAGGGTTGGTGTTTTGATCATGCGCTTAAGTTACGTGATGCCTTACCTGGCGACTGGCTGTGGATTTCGCCGCAGC
CAGATGCTGAAAACCACTGTTCTCCCTCGGCACTACAAACTTTACTTGGGCGCGAGTTCCGGCATGCGGTATTCGACGCC
CGCCACGGCTTTGATGCCGCTGCCTTTGCCGCACTTAGCGGAACGTTGAAAGCGGGAAGCTGGCTGGTTTTGTTACTCCC
TGTATGGGAAGAGTGGGAAAACCAACCTGATGCCGACTCGCTGCGCTGGAGTGATTGCCCTGACCCTATTGCGACGCCGC
ATTTTGTCCAGCATTTCAAACGCGTACTTACGGCGGATAACGACGCTATCTTCTGGCGGCAAAACCAGCCGTTCTCGTTG
GCGCATTTTACTCCCCGTACTGACTGGCACCCCGCGACCGGCGCACCACAACCAGAACAACAGCAAATCTTACAGCAGCT
ACTGACCATGCCGTCGGGCGTGGCAGCGGTAACTGCTGCGCGTGGGCGCGGTAAATCGGCGCTGGCAGGGCAACTCATTT
CTCGTATTGCGGGTAGTGCGATTATCACTGCGCCCGCAAAAGCGGCAACGGATGTACTGGCACAATTTGCGGGCGAGAAG
TTTCGCTTTATTGCGCCTGATGCCTTGTTAGCCAGCGATGAGCAAGCCGACTGGCTGGTGGTCGATGAAGCCGCAGCCAT
ACCTGCGCCGTTGTTGCATCAACTGGTATCGCGTTTTCCTCGAACGTTGTTAACCACTACGGTGCAGGGCTACGAAGGTA
CCGGACGTGGTTTTTTGCTGAAATTTTGCGCTCGCTTTCCGCATTTACACCGTTTTGAACTGCAACAGCCGATCCGCTGG
GCACAGGGATGCCCGCTGGAAAAAATGGTTAGTGAGGCACTGGTTTTTAACGATGAAAACTTCACCCATACACCACAAGG
CAATATCGTCATTTCCGCATTTGAACAAACGTTATGGCGAAGCGAGCCAGAAACGCCGTTAAAGGTTTATCAGTTATTGT
CTGGTGCGCACTACCGGACTTCGCCGCTGGATTTACGCCGCATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGCT
GGCGAAAACGAGATTGCCGGAGCGCTGTGGCTGGTGGATGAGGGGGGATTATCTCAAGAACTCAGTCAGGCGGTATGGGC
AGGTTTTCGTCGCCCGCGGGGTAATCTGGTGGCCCAGTCGCTGGCGGCGCACGGCAGCAATCCACTGGCGGCGACATTGC
GTGGACGGCGGGTCAGCCGGATAGCAGTTCATCCGGCGCGTCAGCGCGAAGGCGTTGGGCAACAGCTCATTGCCAGCGCT
TTGCAATATAGGCCTGGCCTCGACTATCTTTCGGTGAGTTTTGGTTACACCGGGGAGTTATGGCGTTTCTGGCAACGCTG
CGGTTTTGTGCTGGTGCGAATGGGTAATCATCGTGAAGCCAGCAGCGGTTGCTATACGGCGATGGCGCTGTTACCGATGA
GTGATGCGGGTAAACAGCTGGCTGAACGTGAGCATTACCGTTTACGTCGCGATGCGCAAGCTCTCGCGCAGTGGAATGGC
GAAACACTCCCTGTTGATCCACTAAACAATGCCGTCCTTTCTGACGACGACTGGCTTGAACTGGCCGGTTTTGCTTTCGC
TCATCGTCCGCTATTAACATCGTTAGGTTGCTTATTGCGTCTGCTACAAACCAGTGAACTGGCATTACCGGCGCTGCGTG
GGCGTTTACAGAAAAACGTCAGCGACGCGCAGTTATGTACCACACTTAAACTTTCAGGCCGCAAGATGTTACTGGTCCGT
CAGCGGGAAGAGGCCGCACAGGCGCTGTTCGCACTTAATGATGTTCGCACTGAGCGTCTGCGCGATCGCATAACGCAATG
GCAATTTTTTCACTGA

Upstream 100 bases:

>100_bases
CTTCTCAGCAACGCTACAGCTGGTTTAAACGTGGTTTCGACAGCGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGC
ATTTAACTTCCGGGGCAGGG

Downstream 100 bases:

>100_bases
CTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGACATCAGTTAAGGAGTTCGCCATGAAAC
ATGACCATTTTGTTGTTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAGSAIITAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
LQYRPGLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNG
ETLPVDPLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVR
QREEAAQALFALNDVRTERLRDRITQWQFFH

Sequences:

>Translated_671_residues
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAGSAIITAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
LQYRPGLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNG
ETLPVDPLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVR
QREEAAQALFALNDVRTERLRDRITQWQFFH
>Mature_670_residues
AELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDAR
HGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSLA
HFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAGSAIITAPAKAATDVLAQFAGEKF
RFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWA
QGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAG
ENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASAL
QYRPGLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGE
TLPVDPLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVRQ
REEAAQALFALNDVRTERLRDRITQWQFFH

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally si

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=671, Percent_Identity=95.8271236959762, Blast_Score=1314, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507621, Length=372, Percent_Identity=25.2688172043011, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324197, Length=388, Percent_Identity=25.5154639175258, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24640662, Length=450, Percent_Identity=24.2222222222222, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 74792; Mature: 74661

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHC
CCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCHH
QQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAGSAIITAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCCEEHEECCCHHHHCCCHHHHHHHH
LQYRPGLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHCCCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAQALAQWNGETLPVDPLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC
>Mature Secondary Structure 
AELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQPDAENHC
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCHH
QQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAGSAIITAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCCEEHEECCCHHHHCCCHHHHHHHH
LQYRPGLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHCCCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAQALAQWNGETLPVDPLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNVSDAQLCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]