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The map label for this gene is hsrA [H]

Identifier: 209397220

GI number: 209397220

Start: 4835724

End: 4837151

Strand: Reverse

Name: hsrA [H]

Synonym: ECH74115_5189

Alternate gene names: 209397220

Gene position: 4837151-4835724 (Counterclockwise)

Preceding gene: 209398283

Following gene: 209398514

Centisome position: 86.81

GC content: 49.02

Gene sequence:

>1428_bases
ATGAGCGATAAAAAGAAGCGCAGTATGGCGGGTTTGCCGTGGATCGCGGCGATGGCCTTCTTCATGCAGGCACTTGATGC
CACTATTCTGAATACCGCCTTACCCGCAATCGCTCATAGCCTTAATCGTTCTCCTCTCGCGATGCAATCAGCCATCATCA
GTTATACGCTGACGGTGGCGATGCTTATTCCGGTAAGCGGATGGCTAGCCGATCGCTTCGGTACGCGTCGCATTTTTACC
CTTGCCGTGAGTCTGTTCACATTGGGTTCTCTGGCCTGCGCACTTTCTAATTCGCTACCACAGCTGGTTGTCTTCCGGGT
TATTCAGGGGATAGGCGGCGCAATGATGATGCCTGTTGCTCGGCTGGCCTTACTGCGCGCTTATCCTCGTAATGAACTTC
TTCCAGTATTGAATTTTGTCGCCATGCCGGGTCTGGTGGGGCCAATTTTAGGCCCCATTCTTGGCGGCGTGCTGGTCACC
TGGGCAACCTGGCACTGGATATTTTTAATCAATATCCCCATAGGTATTGCGGGCCTTCTTTACGCGCGCAAACATATGCC
CAATTTCACCACCGCACGACGCAGATTCGATATCACTGGCTTTTTGCTGTTTGGCCTCAGCCTTGTTCTCTTCTCAAGCG
GAATAGAGCTATTCGGGGAAAAGATTGTCGCCAGCTGGATTGCCTTGACGGTAATTGTCACCAGCATCGGGTTACTGCTT
CTCTATATTCTCCATGCACGACGCACGCCAAACCCATTAATTTCATTAGATTTATTTAAAACCCGCACTTTCTCGATCGG
TATCGTAGGCAATATTGCAACCCGTCTGGGGACCGGCTGTGTACCGTTCCTTATGCCATTAATGTTACAGGTAGGATTTG
GTTATCAGGCGTTTATTGCTGGCTGTATGATGGCACCGACAGCGTTAGGTTCCATTATTGCAAAATCGATGGTTACCCAA
GTCTTACGTCGTCTGGGCTATCGCCATACATTAGTGGGGATCACGGTGATTATTGGGCTAATGATCGCTCAGTTCTCTTT
GCAATCACCGGCAATGGCAATATGGATGCTGATCTTGCCGTTGTTTATATTAGGGATGGCTATGTCGACGCAGTTTACCG
CGATGAATACCATCACACTTGCCGATCTGACCGATGACAACGCCAGCAGCGGTAACAGTGTTCTGGCGGTCACGCAGCAA
CTGTCTATCAGTTTAGGCGTAGCTGTAAGTGCGGCCGTCCTTCGCGTTTATGAAGGAATGGAAGGCACAACGACTGTCGA
ACAATTCCACTATACGTTTATCACAATGGGCATTATTACTGTTGCTTCAGCAGCAATGTTCATGCTTCTGAAAACAACCG
ATGGTAATAATTTGATCAAAAGACAGCGTAAATCTAAGCCGAACCACGTTCCATCAGAATCGGAGTAA

Upstream 100 bases:

>100_bases
GTCGATGCGATTATCCAAAGCGATGGCGACGCGGCATTTAAAGCTTGTCAGGCGCTGCTACGCTCACCTGATAAGTGATA
ACCCGATAACAGGACATTGA

Downstream 100 bases:

>100_bases
GTTGTAATCGTTGTTGCTGAAGGGTCGGCTGGGTTATCCGATGGATGAGTACATCAATCGCCAGCTCCCCCAGTTCATCT
TTTGGTTGATGGATCGTGGT

Product: drug resistance MFS transporter, drug:H+ antiporter-1 (DHA2) family

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFT
LAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQ
VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNHVPSESE

Sequences:

>Translated_475_residues
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFT
LAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQ
VLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQ
LSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNHVPSESE
>Mature_474_residues
SDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTL
AVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVTW
ATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLL
YILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQV
LRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQL
SISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNHVPSESE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=475, Percent_Identity=99.5789473684211, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=459, Percent_Identity=45.5337690631808, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=32.3943661971831, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=32.4942791762014, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=28.5024154589372, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=30.1470588235294, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=473, Percent_Identity=26.0042283298097, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.9727891156463, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=32.2834645669291, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=402, Percent_Identity=24.1293532338308, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51458; Mature: 51326

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVTWATWHWIFLINIPIGIAGLL
HHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHH
YARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIA
HHHHHHCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
GCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGM
HHHHHHHHHCHHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
EGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNHVPSESE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGGAMMMPVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVTWATWHWIFLINIPIGIAGLL
HHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHH
YARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIA
HHHHHHCCCCCCCEEEEEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
GCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRVYEGM
HHHHHHHHHCHHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCC
EGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSKPNHVPSESE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]