Click here to switch to the map view.

The map label for this gene is hemG

Identifier: 209397190

GI number: 209397190

Start: 4933843

End: 4934388

Strand: Direct

Name: hemG

Synonym: ECH74115_5289

Alternate gene names: 209397190

Gene position: 4933843-4934388 (Clockwise)

Preceding gene: 209397794

Following gene: 209399915

Centisome position: 88.55

GC content: 52.2

Gene sequence:

>546_bases
GTGAAAACATTAATTCTTTTCTCAACAAGGGACGGACAAACGCGCGAGATTGCCTCCTACTTGGCTTCGGAACTGAAAGA
ACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTACGACCGTGTGGTCATTG
GTGCTTCTATTCGCTATGGTCACTACCACTCTGCGTTCCAGGAATTTGTCAAAAAGCATGCGACGCGGCTGAATTCGATG
CCGAGCGCGTTTTACTCCGTAAACCTGGTGGCGCGTAAACCGGAGAAGCGCACTCCACAGACCAACAGCTACGCGCGGAA
GTTTCTGATGAACTCGCAATGGCGTCCCGATCGCTGCGCGGTCATTGCCGGGGCGCTGCGTTACCCACGTTATCGCTGGT
ACGACCGTTTTATGATCAAGCTGATTATGAAGATGTCAGGCGGTGAAACGGATACGCGCAAAGAAGTTGTCTATACCGAT
TGGGAGCAGGTGGCGAATTTCGCCCGAGAAATCGCCCATTTAACCGACAAACCGACGCTGAAATAA

Upstream 100 bases:

>100_bases
AATGGATCCTGATTGCCAACATGCTGTTTGGTCGTCTCGAGGTCTTTACATTGCTGGTGCTCTTTACCCCGACTTTCTGG
CGTGAATGATGGAGTAATAC

Downstream 100 bases:

>100_bases
GCATAAAGAATAAAAAATGCGCGGTCAGAAAATTATTTTAAATTTCCCCTTGTCAGGCCGGAATAACTCCCTATAATGCG
CCACCACTGACACGGAACAA

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQWENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSM
PSAFYSVNLVARKPEKRTPQTNSYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
WEQVANFAREIAHLTDKPTLK

Sequences:

>Translated_181_residues
MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQWENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSM
PSAFYSVNLVARKPEKRTPQTNSYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
WEQVANFAREIAHLTDKPTLK
>Mature_181_residues
MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQWENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSM
PSAFYSVNLVARKPEKRTPQTNSYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
WEQVANFAREIAHLTDKPTLK

Specific function: Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX using menaquinone as electron acceptor

COG id: COG4635

COG function: function code CH; Flavodoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Escherichia coli, GI1790285, Length=181, Percent_Identity=100, Blast_Score=382, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMG_ECO57 (P0ACB6)

Other databases:

- EMBL:   AB035920
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91226
- PIR:   H86072
- RefSeq:   NP_290480.1
- RefSeq:   NP_312805.1
- ProteinModelPortal:   P0ACB6
- EnsemblBacteria:   EBESCT00000025510
- EnsemblBacteria:   EBESCT00000060471
- GeneID:   915124
- GeneID:   960310
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5372
- KEGG:   ecs:ECs4778
- GeneTree:   EBGT00050000010479
- HOGENOM:   HBG540004
- OMA:   EIEYTDW
- ProtClustDB:   PRK11104
- BioCyc:   ECOL83334:ECS4778-MONOMER
- InterPro:   IPR008254
- InterPro:   IPR001226

Pfam domain/function: PF00258 Flavodoxin_1

EC number: =1.3.5.3

Molecular weight: Translated: 21227; Mature: 21227

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00201 FLAVODOXIN; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQWENYDRVVIGASIRYG
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEEEECEECCC
HYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTNSYARKFLMNSQWRPDRCA
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHH
VIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTDWEQVANFAREIAHLTDKPTL
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCCC
K
C
>Mature Secondary Structure
MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQWENYDRVVIGASIRYG
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEEEECEECCC
HYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTNSYARKFLMNSQWRPDRCA
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHH
VIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTDWEQVANFAREIAHLTDKPTL
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796