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The map label for this gene is rhtB

Identifier: 209397127

GI number: 209397127

Start: 4907864

End: 4908484

Strand: Reverse

Name: rhtB

Synonym: ECH74115_5265

Alternate gene names: 209397127

Gene position: 4908484-4907864 (Counterclockwise)

Preceding gene: 209399445

Following gene: 209398393

Centisome position: 88.09

GC content: 51.53

Gene sequence:

>621_bases
ATGACCTTAGAATGGTGGTTTGCCTACCTACTGACATCAATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACAC
TATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTCTATTGCCGGGCTTCAGACCGGACTGGCGATTCATA
TTGTGCTGGTTGGCGTGGGGTTGGGGACGCTATTTTCCCGCTCAGTGATTGCGTTTGAAGTGTTGAAGTGGGCAGGCGCG
GCTTACTTGATTTGGCTGGGAATACAGCAATGGCGCGCCGCTGGCGCAATTGATCTTAAATCGCTGGCCTCTACTCAATC
GCGTCGACATTTGTTCCAGCGCGCAGTTTTTGTGAATCTCACCAATCCCAAAAGTATTGTGTTTCTGGCGGCGCTATTTC
CGCAATTCATCATGCCGCAACAGCCGCAACTGATGCAGTATATCGTGCTCGGTGTCACCACTATTGTGGTCGATATTATT
GTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAGGCGCTGAATAA
GATTTTCGGCTCGTTGTTTATGCTGGTGGGAGCGCTGTTAGCATCGGCGAGGCATGCGTGA

Upstream 100 bases:

>100_bases
GCCTCTTAAACCACGTAAAATCGTTTTTTTTAGCGTGCCTGACACAACGCTGCGACAGTAGCGTATTGTGGCACAAAAAT
AGACACACCGGGAGTTCATC

Downstream 100 bases:

>100_bases
AAAATAATGTCGGATGCGGCGTAAACGCCTTATCCGACTTACTCTGAAGACGCGTCTGGCATTACCGCGAAATAATCAAA
TGAATGCCAAATCCGGCAAA

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGA
AYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA

Sequences:

>Translated_206_residues
MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGA
AYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA
>Mature_205_residues
TLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAA
YLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIV
MIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA

Specific function: Conducts the efflux of homoserine and homoserine lactone

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=100, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1788099, Length=204, Percent_Identity=27.4509803921569, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHTB_ECO57 (P0AG36)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91223
- RefSeq:   NP_290456.1
- RefSeq:   NP_312781.1
- ProteinModelPortal:   P0AG36
- EnsemblBacteria:   EBESCT00000028845
- EnsemblBacteria:   EBESCT00000056206
- GeneID:   915150
- GeneID:   960335
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5345
- KEGG:   ecs:ECs4754
- GeneTree:   EBGT00050000008904
- HOGENOM:   HBG759042
- OMA:   QWRARVE
- BioCyc:   ECOL83334:ECS4754-MONOMER
- InterPro:   IPR004778
- InterPro:   IPR001123
- TIGRFAMs:   TIGR00949

Pfam domain/function: PF01810 LysE

EC number: NA

Molecular weight: Translated: 22427; Mature: 22296

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13183448)-; HASH(0x12f1b228)-; HASH(0x12a3c0c4)-; HASH(0x133a837c)-; HASH(0x1319b18c)-; HASH(0x131a0570)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
TNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLAQRIALWIKGPK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKIFGSLFMLVGALLASARHA
HHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
TLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
TNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLAQRIALWIKGPK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKIFGSLFMLVGALLASARHA
HHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796