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The map label for this gene is hisIE

Identifier: 209397099

GI number: 209397099

Start: 2733514

End: 2734125

Strand: Direct

Name: hisIE

Synonym: ECH74115_2959

Alternate gene names: 209397099

Gene position: 2733514-2734125 (Clockwise)

Preceding gene: 209397885

Following gene: 209400062

Centisome position: 49.06

GC content: 53.27

Gene sequence:

>612_bases
ATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTGATGCCGGTGATTGTGCAACACGCGGT
ATCCGGCGAAGTGTTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCATCGAAAGCGGCAAAGTCACCTTCT
TCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTAAATATTGCGCCGGAC
TGCGACAACGACACGCTACTGGTACTGGCGAATCCTATCGGACCAACCTGCCACAAAGGCACCAGCAGCTGCTTCGGCGA
CACCGCTCACCAGTGGCTGTTCCTGTATCAACTGGAACAATTGCTCGCCGAACGCAAATCTGCCGACCCGGAAACCTCCT
ACACCGCCAAACTGTATGCCAGCGGCACCAAACGCATTGCGCAGAAAGTGGGCGAAGAAGGCGTGGAAACCGCACTGGCC
GCGACTGTACATGACCGCTTTGAGCTGACCAACGAGGCGTCTGACTTGATGTATCACCTGCTGGTATTGTTGCAGGATCA
GGATCTGGATTTAACAACGGTGATTGAGAACCTGCGTAAACGTCATCAGTGA

Upstream 100 bases:

>100_bases
ACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACA
CAGGGCGTGGAGATCAGGAT

Downstream 100 bases:

>100_bases
GTTGCGGGGTAAGCGGATGCGATATTGTTGTCGCATCCGGCAAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTC
TTTAAAACCGAAAAGATTAC

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ

Sequences:

>Translated_203_residues
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ
>Mature_203_residues
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=203, Percent_Identity=98.5221674876847, Blast_Score=417, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319819, Length=204, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_ECO57 (Q9S5G3)

Other databases:

- EMBL:   AB008676
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85828
- PIR:   C90982
- RefSeq:   NP_288531.1
- RefSeq:   NP_310854.1
- ProteinModelPortal:   Q9S5G3
- EnsemblBacteria:   EBESCT00000026449
- EnsemblBacteria:   EBESCT00000060272
- GeneID:   912426
- GeneID:   962084
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3188
- KEGG:   ecs:ECs2827
- GeneTree:   EBGT00050000011321
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BioCyc:   ECOL83334:ECS2827-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 22841; Mature: 22841

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQR
CCCHHHHHCCCCHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEHHHHHHH
LWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQ
HHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHHHH
LLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHL
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLQDQDLDLTTVIENLRKRHQ
HHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQR
CCCHHHHHCCCCHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEHHHHHHH
LWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTSSCFGDTAHQWLFLYQLEQ
HHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHCHHHHHHHHHHHHHH
LLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHL
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLQDQDLDLTTVIENLRKRHQ
HHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796