Click here to switch to the map view.

The map label for this gene is pphA [H]

Identifier: 209397009

GI number: 209397009

Start: 2430438

End: 2431094

Strand: Reverse

Name: pphA [H]

Synonym: ECH74115_2571

Alternate gene names: 209397009

Gene position: 2431094-2430438 (Counterclockwise)

Preceding gene: 209397388

Following gene: 209397373

Centisome position: 43.63

GC content: 48.1

Gene sequence:

>657_bases
ATGAAACAGCCTGCGCCCGTTTATCAGAGAATTGCGGGTCATCAATGGCGACATATCTGGCTTTCTGGCGATATACACGG
TTGTCTTGAGCAGTTGCGCCGCAAATTATGGCATTGTCGTTTTGATCCGTGGCGAGATTTACTTATCTCAGTGGGAGACG
TTATCGATCGTGGGCCGCAAAGTTTACGTTGTCTGCAGTTACTGGAACAACATTGGGTTCGTGCGGTAAGAGGCAATCAT
GAACAGATGGCGATGGATGCGCTGGCATCCCAGCAGATGTCTTTGTGGTTGATGAATGGCGGCGACTGGTTTATTGCGCT
GGCAGATAATCAACAGAAACAAGCGAAAACGGCGCTGGAAAAATGTCAGCATTTGCCCTTTATTCTTGAAGTACACAGCC
GCACCGGCAAACATGTTATTGCTCATGCCGATTATCCAGATGATGTTTATGAATGGCAAAAGGACGTTGATTTGCATCAG
GTCTTGTGGAGCCGCTCGCGATTAAGTGAACGCCAAAAAGGGCAGGGAATTACAGGTGCTGATCATTTCTGGTTTGGTCA
TACACCGTTGCGCCATCGCGTGGATATTGGCAACCTGCATTATATTGATACCGGCGCTGTCTTTGGGGGCGAACTGACTT
TCGTGCAATTGCAATAA

Upstream 100 bases:

>100_bases
CTTTATAGTCAACCATAACACACACTCTACTGTCTGAGTCCAGCGTTTTTTAACATTCTTGTTAAGATTATGTGATCTTT
AGCGCGGGAGGAAAATATTG

Downstream 100 bases:

>100_bases
TTAAAAATCACCATACTCCTGTGCTGGTCGCCAGAAACCATCTATAAAATCCTCAATCGGAAAACAACCGCCATGACGGA
TCCGTTGATCGCTCATAGAA

Product: serine/threonine protein phosphatase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ

Sequences:

>Translated_218_residues
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
>Mature_218_residues
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=218, Percent_Identity=98.6238532110092, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789090, Length=217, Percent_Identity=44.2396313364055, Blast_Score=190, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25391; Mature: 25391

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQ
CCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHH
SLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCEEEEECCCCHHHHHHHHH
KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLSERQKGQGITGA
HHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
CCEEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC
>Mature Secondary Structure
MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQ
CCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHH
SLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCEEEEECCCCHHHHHHHHH
KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLSERQKGQGITGA
HHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
CCEEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]