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The map label for this gene is arrQ [H]

Identifier: 209396957

GI number: 209396957

Start: 2599686

End: 2600219

Strand: Reverse

Name: arrQ [H]

Synonym: ECH74115_2785

Alternate gene names: 209396957

Gene position: 2600219-2599686 (Counterclockwise)

Preceding gene: 209397622

Following gene: 209396218

Centisome position: 46.67

GC content: 54.87

Gene sequence:

>534_bases
ATGAACGCAAAAATCAGATACGGCCTGTCGGCTGCCGTTCTGGCGCTGATTGGTGCAGGGGCGTCTGCGCCTGAAATCCT
CGACCAGTTTCTTGACGAAAAAGAAGGTAACCACACCACGGCATACCGTGATGGTGCGGGTATCTGGACCATCTGCCGCG
GTGCCATCCTGGTGGATGGTAAACCTGTCGTTCCGGGCATGAAGTTGTCGAAGGAAAAATGCGACCAGGTTAACGCCATT
GAACGTGATAAGGCGCTGGAGTGGGTGGAGCGCAATATTAAAGTACCGCTGACCGAACCCCAGAAAGCGGGGATCGCGTC
ATTCTGTCCGTACAACATTGGTCCCGGTAAGTGTTTCCCGTCGACGTTTTACAGACGAATTAATGCAGGTGATCGCAGGG
GAGCGTGTGAGGCGATTCGCTGGTGGATTAAGGACGGTGGCAGAGACTGCCGTATTCGCTCAAACAACTGCTACGGTCAG
GTTTCACGGCGTGACCAGGAGAGCGCGCTGGCGTGCTGGGGAATTGACAGATAA

Upstream 100 bases:

>100_bases
AACTACCGGGAATAATTACGGGTTCTCTGAAGACTCCACCGCTGGCCTGAAGACTTAACATATCCAGGGATTTGAAATCG
ATAAACCCTGATAAATATCC

Downstream 100 bases:

>100_bases
GCAGAATATTTTGCTGAAAAATGCGGTTTGCTCACACTGGCGGATAACACGAAATCCTGCGAACTGGCAAAAACTAAGTG
AATAAAAGTAAAACCCCGTT

Product: phage lysozyme

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAI
ERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
VSRRDQESALACWGIDR

Sequences:

>Translated_177_residues
MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAI
ERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
VSRRDQESALACWGIDR
>Mature_177_residues
MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAI
ERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
VSRRDQESALACWGIDR

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1787836, Length=177, Percent_Identity=90.3954802259887, Blast_Score=338, Evalue=1e-94,
Organism=Escherichia coli, GI1786768, Length=143, Percent_Identity=31.4685314685315, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 19577; Mature: 19577

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAILVDG
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCEEEECC
KPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCH
STFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAILVDG
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCEEEECC
KPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCH
STFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]