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The map label for this gene is ycaN [H]

Identifier: 209396927

GI number: 209396927

Start: 354907

End: 355833

Strand: Direct

Name: ycaN [H]

Synonym: ECH74115_0347

Alternate gene names: 209396927

Gene position: 354907-355833 (Clockwise)

Preceding gene: 209399065

Following gene: 209399148

Centisome position: 6.37

GC content: 43.91

Gene sequence:

>927_bases
ATGATGAAAATAGAGCCTTCAATTTTGCCTTCTCTTGCCTGGTTTGCGCTGATTGTTCGTGCCGGCAGTTTTTCCAGAGC
CGCTTCTGAAATGGGTATTACGCTGGCCGCATTGTCCCAGAATTTAAAATCTCTTGAGGAACGTCTTAACACCAAGCTGA
TATACCGTACGACCCGCAATATCTCGCTTACTGAGGAAGGACAGCGTCTGTATGAAGTGCTGGTGTCGGCTTTGGGGCAA
ATTGATGATGCTTTGAAAGATGTTGGTGATACCCAACTTGAACCTACAGGCCTGTTGAGAATAAATTCCTCCCGTGTTGC
AGCACGGATGCTTGTTGAGCCTCATATTGGCGAGTTTCTTACTCGTTACCCCAAAACAAAGATAGAACTCATTATGGATG
ACGGTTTATCTAACATTATTGCGGACGGCTGTGATGTGGGCATACGTCTTGAGCAAGGACTGGATGAGCATATGACTGCC
GTTCCTGTTTCTCCGCTTATTAAACTGGTTACTGTGGCATCGCCAGATTATTTAAAAGAACACGGTATTCCTGAAACGCC
TCAGGAACTGAGTAACCATAATTGCCTTCGGTTGCGGCATAAAAGTAGCGGTGCACTCTCTGCATGGGAGTTTTCTAAGG
TTGTGGGGGGTAATGAAGAGTTCGAAATAGAGGTTTCAGGTAAGTATATTTCTAATGATGACGAAAGCATGATACGAATG
GCGCTGAATGGAACGGGTATCATTCAACATCTGGATTTTGCAATCGCTGAACATATCAATGCGGGCAAGTTACAGCCGAT
TCTTGAGGATTGGGCTGTTTCATTTCCTGGGTTTTATATTTATGTTTCATCACGGGTGAGAATGCCCTCTAAAGTTCGTG
CTTTTATTGATTTTATGGTGGAGAAAAGAGTAAAACTCGAAAATTGA

Upstream 100 bases:

>100_bases
CTGGCATTTGTGTTCATATCGAGAACGGCGTAATAGTATGACTTGTATTGGTTATCATCAATGGTCTAATATCTATCACA
GTGTTAAGTTAAGGTTTACA

Downstream 100 bases:

>100_bases
TGATATGTGTAATTGATGGTTGCTCTGGGCGAATATCGGTAAGAGGAATAACGTAATCATTAGGATGCGTTTACATAATA
AAGTTTCTGATAATTAATAC

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKSLEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQ
IDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGNEEFEIEVSGKYISNDDESMIRM
ALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN

Sequences:

>Translated_308_residues
MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKSLEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQ
IDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGNEEFEIEVSGKYISNDDESMIRM
ALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN
>Mature_308_residues
MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKSLEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQ
IDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGNEEFEIEVSGKYISNDDESMIRM
ALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=38.4879725085911, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=31.1643835616438, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=34.2465753424658, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1789440, Length=282, Percent_Identity=30.4964539007092, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=28.4246575342466, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=27.6094276094276, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=28.0821917808219, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=24.3986254295533, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790011, Length=300, Percent_Identity=24.3333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=159, Percent_Identity=27.0440251572327, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=28.1045751633987, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=23.1578947368421, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34312; Mature: 34312

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKSLEERLNTKLIYRTTRN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCC
ISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFL
CEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHH
TRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTAVPVSPLIKLVTVASPDYLKE
HHCCCCEEEEEECCCHHHHHHCCCCCCEEHHCCCHHHHHHCCHHHHHHHHHCCCCHHHHH
HGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGNEEFEIEVSGKYISNDDESMIRM
CCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCEECCCHHHHHHH
ALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMPSKVRAFIDFMV
HHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
EKRVKLEN
HHHHCCCC
>Mature Secondary Structure
MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKSLEERLNTKLIYRTTRN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCC
ISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRINSSRVAARMLVEPHIGEFL
CEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCHHHHHH
TRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTAVPVSPLIKLVTVASPDYLKE
HHCCCCEEEEEECCCHHHHHHCCCCCCEEHHCCCHHHHHHCCHHHHHHHHHCCCCHHHHH
HGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGNEEFEIEVSGKYISNDDESMIRM
CCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCEECCCHHHHHHH
ALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFPGFYIYVSSRVRMPSKVRAFIDFMV
HHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
EKRVKLEN
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]