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The map label for this gene is eptA

Identifier: 209396834

GI number: 209396834

Start: 5263596

End: 5265239

Strand: Reverse

Name: eptA

Synonym: ECH74115_5627

Alternate gene names: 209396834

Gene position: 5265239-5263596 (Counterclockwise)

Preceding gene: 209396251

Following gene: 209397598

Centisome position: 94.49

GC content: 49.88

Gene sequence:

>1644_bases
ATGTTGAAGCGCCTACTAAAAAGACCCTCTTTGAATTTACTCGCCTGGCTATTGTTGGCCGCTTTTTATATCTCTATCTG
CCTGAATATTGCCTTTTTTAAACAGGTGTTGCAGGCGCTGCCGCTGGACTCGCTGCATAACGTACTGGTTTTCTTGTCGA
TGCCGGTCGTAGCCTTCAGCGTGATTAATATTGTCCTGACACTAAGCTCTTTCTTATGGCTTAATCGACCGCTGGCCTGC
CTGTTTATTCTGGTTGGCGCGGCTGCACAATATTTCATAATGACTTACGGCATCGTCATCGACCGCTCGATGATTGCCAA
TATTATTGATACCACTCCGGCAGAAAGCTATGCGCTGATGACACCGCAAATGTTATTAACGCTGGGATTCAGCGGCGTGC
TTGCTGCGCTGATTGCCTGCTGGATTAAAATCAAACCTGCCACCTCGCGTCTGCGCAGTGTTCTTTTCCGTGGAGCCAAT
ATTCTGGTTTCTGTACTGCTGATTTTGCTGGTCGCCGCACTATTTTATAAAGACTACGCCTCGTTGTTCCGCAACAACAA
AGAGCTGGTGAAGTCCTTAAGCCCCTCTAACAGCATTGTTGCCAGCTGGTCATGGTACTCCCATCAGCGACTGGCAAATC
TGCCGCTGGTGCGAATTGGTGAAGACGCGCACCGCAACCCATTAATGCAGAACGAAAAACGTAAAAATTTGACCATCCTG
ATTGTCGGCGAAACCTCGCGGGCGGAGAACTTCTCCCTCAACGGCTACCCGCGTGAAACTAACCCGCGGTTGGCGAAAGA
TAACGTGGTCTATTTCCCTAATACCGCATCTTGCGGCACGGCAACGGCAGTTTCAGTACCGTGCATGTTCTCGGATATGC
CGCGAGAGCACTACAAAGAAGAGCTGGCACAGCACCAGGAAGGCGTGCTGGATATCATTCAGCGAGCGGGCATCAACGTG
CTGTGGAATGACAACGATGGCGGCTGTAAAGGTGCCTGCGACCGCGTGCCTCACCAGAACGTCACCGCGCTGAATCTGCC
TGATCAGTGCATCAACGGCGAATGCTATGACGAAGTGCTGTTCCACGGGCTGGAAGAGTACATCAATAACCTGCAGGGTG
ATGGCGTGATTGTCTTACACACCATCGGCAGCCACGGTCCGACCTATTACAACCGCTATCCGCCGCAGTTCAGGAAATTT
ACCCCAACCTGCGACACCAATGAGATCCAGACCTGTACCAAAGAGCAACTGGTGAACACTTACGACAACACGCTGGTTTA
CGTCGACTATATTGTTGATAAAGCGATTAATCTGCTGAAAGAACATCAGGATAAATTTACCACCAGCCTGGTTTATCTTT
CTGACCACGGTGAATCGTTAGGTGAAAATGGCATCTATCTGCACGGTCTGCCTTATGCCATCGCCCCGGATAGCCAAAAA
CAGGTGCCGATGCTGCTGTGGCTGTCGGAGGATTATCAAAAACGGTATCAGGTTGACCAGAACTGCCTGCAAAAACAGGC
GCAAACGCAACACTATTCACAAGACAATTTATTCTCCACGCTATTGGGATTAACTGGCGTTGAGACGAAGTATTACCAGG
CTGCGGATGATATTCTGCAAACTTGCAGGAGAGTGAGTGAATGA

Upstream 100 bases:

>100_bases
CCCTTAATCCAGCAAAGATAAAAGCCAACCTTAAGAACTTAAGGTTGGCTTAATTTTGCTTTGCGAGCATATGCGCACTT
TGTTCGATGGAAACACCGTG

Downstream 100 bases:

>100_bases
AAATTCTGATTGTTGAAGACGATACGCTGTTATTGCAGGGACTGATTCTGGCGGCGCAAACCGAAGGCTACGCGTGCGAT
GGCGTGACAACCGCGCGGAT

Product: putative cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLAC
LFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTIL
IVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINV
LWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKF
TPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQK
QVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE

Sequences:

>Translated_547_residues
MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLAC
LFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTIL
IVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINV
LWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKF
TPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQK
QVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE
>Mature_547_residues
MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLAC
LFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTIL
IVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINV
LWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKF
TPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQK
QVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily

Homologues:

Organism=Escherichia coli, GI87082372, Length=547, Percent_Identity=100, Blast_Score=1135, Evalue=0.0,
Organism=Escherichia coli, GI87082286, Length=546, Percent_Identity=24.5421245421245, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082223, Length=462, Percent_Identity=24.8917748917749, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPTA_ECOLI (P30845)

Other databases:

- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   D14055
- RefSeq:   AP_004615.1
- RefSeq:   NP_418538.2
- ProteinModelPortal:   P30845
- DIP:   DIP-12561N
- IntAct:   P30845
- MINT:   MINT-1218890
- STRING:   P30845
- EnsemblBacteria:   EBESCT00000004410
- EnsemblBacteria:   EBESCT00000015212
- GeneID:   948629
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5730
- KEGG:   eco:b4114
- EchoBASE:   EB1570
- EcoGene:   EG11613
- eggNOG:   COG2194
- GeneTree:   EBGT00050000009020
- HOGENOM:   HBG478687
- OMA:   IDRSMIT
- ProtClustDB:   PRK11598
- BioCyc:   EcoCyc:EG11613-MONOMER
- Genevestigator:   P30845
- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917
- Gene3D:   G3DSA:3.40.720.10

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase; SSF53649 Alkaline_phosphatase_core

EC number: NA

Molecular weight: Translated: 61668; Mature: 61668

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x138e5350)-; HASH(0x11e0e08c)-; HASH(0x13a6960c)-; HASH(0x137b621c)-; HASH(0x136746b8)-;

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFS
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
TPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGANILVSVLLILLVAALFYKDYA
HHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTIL
HHHHCCHHHHHHCCCCCCEEEEECHHHHHHHHCCCEEEECCCHHCCCCCCCCCCCCEEEE
IVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKE
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHEECCHHHHCCCHHHHHH
ELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCEEEECCCHHHCCCCHHHHHH
FHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQTCTKEQLVNT
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHH
YDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQK
HCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECCCCEECCCCCC
QVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQ
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
TCRRVSE
HHHHHCH
>Mature Secondary Structure
MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFS
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
TPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGANILVSVLLILLVAALFYKDYA
HHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTIL
HHHHCCHHHHHHCCCCCCEEEEECHHHHHHHHCCCEEEECCCHHCCCCCCCCCCCCEEEE
IVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKE
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHEECCHHHHCCCHHHHHH
ELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVL
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCCEEEECCCHHHCCCCHHHHHH
FHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQTCTKEQLVNT
HHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHH
YDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQK
HCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECCCCEECCCCCC
QVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQ
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
TCRRVSE
HHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725