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The map label for this gene is hyfR [H]

Identifier: 209396720

GI number: 209396720

Start: 3432160

End: 3434172

Strand: Direct

Name: hyfR [H]

Synonym: ECH74115_3713

Alternate gene names: 209396720

Gene position: 3432160-3434172 (Clockwise)

Preceding gene: 209399076

Following gene: 209397824

Centisome position: 61.6

GC content: 52.76

Gene sequence:

>2013_bases
ATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCGCAAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCAGAGCG
GTTAGCGCAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTA
TTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTAT
CAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCAC
CCACGGCAGTCGTTTTACTCCCCAGGATTGCACACTGTTTAACTGGCTGGCACGCATTATCACCCCGGTTCTGCAATCAT
GGCTCAATGATGAAGAACAACAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTGGATATC
ACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCT
GGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGCAGCGATCTTTCTGCCTCAC
ATTGTGCGTGTCTGCCACGCAATATGCCTGGCGACAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTG
ACGCACCGTGCAGACGATCTGTTTCTCTGGCAACGCGACCCGTTATTACACTTACTTGCATCTAACGGCTGCGAATCTTC
GCTCCTGATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTACCACTCTCTTTAGTG
AGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATG
ACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCGTCGGTGACAT
TATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGGTAGATATTGTGGCGAAGAGCGACAGTACGGTGTTGATTT
GTGGTGAAACCGGAACCGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTC
AAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCCTTTACTGGTGC
GATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGTACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAG
AACTTCAGCCTAAATTGCTGCGCGTATTGCAGGAGCGGGAAATTGAGCGTCTCGGCGGGAGTAGAACGATCCCGGTGAAT
GTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCG
CCTGAATGTCTTCCCACTGGAATTGCCGCCGCTGCGAGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGC
AAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGG
CCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGAGCGGTACTGCTGACTCGCGGTAACAGTCTGAATTTACATCT
TAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTGCTGCACC
CGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGG
CCCCGTGGCGCGGCGACACGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGTCTGGGGATCTCGGTTCG
CGAGGTGTTGTAA

Upstream 100 bases:

>100_bases
AATTCATTCATCTGTTGGATGAAATTGTGCTGGAACCGGCTATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTT
CTCATTAATAAGGACTGTTG

Downstream 100 bases:

>100_bases
TCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCTGA
ACGGATTGCCGCCCATAAAA

Product: formate hydrogenlyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEY
QGEMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTL
THRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGNHTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSM
TDLQESLQQENHQLSEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLV
KINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVN
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDW
PGNVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAG
PRGAATRLGMKRTTLLSRMQRLGISVREVL

Sequences:

>Translated_670_residues
MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEY
QGEMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTL
THRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGNHTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSM
TDLQESLQQENHQLSEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLV
KINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVN
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDW
PGNVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAG
PRGAATRLGMKRTTLLSRMQRLGISVREVL
>Mature_669_residues
AMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQ
GEMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDIT
NAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTLT
HRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGNHTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMT
DLQESLQQENHQLSEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVK
INCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVNV
RVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWP
GNVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGP
RGAATRLGMKRTTLLSRMQRLGISVREVL

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=670, Percent_Identity=98.955223880597, Blast_Score=1358, Evalue=0.0,
Organism=Escherichia coli, GI1789087, Length=573, Percent_Identity=50.7853403141361, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1788550, Length=353, Percent_Identity=48.1586402266289, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1790437, Length=315, Percent_Identity=48.2539682539683, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=55.1724137931034, Blast_Score=264, Evalue=2e-71,
Organism=Escherichia coli, GI1790299, Length=364, Percent_Identity=42.3076923076923, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=54.8936170212766, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=42.4615384615385, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1786524, Length=355, Percent_Identity=42.5352112676056, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=49.3506493506493, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=36.5325077399381, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=35.2941176470588, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789828, Length=237, Percent_Identity=37.5527426160338, Blast_Score=143, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 75353; Mature: 75222

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHEEEC
SNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLF
CCCEEEECCCCCCEEEEECCCCCEECCCCCEEEEECCCCHHEEEEEECCCCCCCCCHHHH
NWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVARE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHH
IHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTL
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHEECCCCCCCCCEEEEHHHHCCCCCH
THRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGNHTPGALLLAHTSTTLFSEENCQLL
HCCCCCEEEECCCHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEECCHHEECCCHHHHH
QHIADRIAIAVGNADAWRSMTDLQESLQQENHQLSEQLLSNLGVGDIIYQSQAMEDLLQQ
HHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHD
HHHEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHHHHCCC
KGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVN
CCCEEECCCCCCCEEEECCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHM
EEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
NRAIDAIPTEALRQLMSWDWPGNVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNE
HHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCC
DSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHH
RLGISVREVL
HHCCHHHHCC
>Mature Secondary Structure 
AMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHEEEC
SNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLF
CCCEEEECCCCCCEEEEECCCCCEECCCCCEEEEECCCCHHEEEEEECCCCCCCCCHHHH
NWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVARE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHH
IHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTL
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHEECCCCCCCCCEEEEHHHHCCCCCH
THRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGNHTPGALLLAHTSTTLFSEENCQLL
HCCCCCEEEECCCHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEECCHHEECCCHHHHH
QHIADRIAIAVGNADAWRSMTDLQESLQQENHQLSEQLLSNLGVGDIIYQSQAMEDLLQQ
HHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHD
HHHEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHHHHCCC
KGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVN
CCCEEECCCCCCCEEEECCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHM
EEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
NRAIDAIPTEALRQLMSWDWPGNVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNE
HHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCC
DSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHH
RLGISVREVL
HHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]