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The map label for this gene is emrA [H]

Identifier: 209396692

GI number: 209396692

Start: 3639809

End: 3640993

Strand: Direct

Name: emrA [H]

Synonym: ECH74115_3930

Alternate gene names: 209396692

Gene position: 3639809-3640993 (Clockwise)

Preceding gene: 209400865

Following gene: 209396264

Centisome position: 65.32

GC content: 53.25

Gene sequence:

>1185_bases
GTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCT
GCTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCG
AAGAAACCGATGACGCATACGTGGCAGGGAATCAAATTCAAATTATGTCTCAGGTGTCTGGCAGCGTGACGAAAGTCTGG
GCCGATAACACCGATTTTGTAAAAGAAGGCGACGTGCTGGTCACTCTCGACCCGACAGATGCTCGCCAGGCGTTTGAAAA
AGCCAAAACTGCACTGGCTTCCAGCGTTCGCCAAACCCACCAGCAGATGATTAACAGCAAGCAGTTGCAGGCGAATATTG
AGGTGCAAAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGT
CGCGAAGAGCTGCAACACGCCCGCGACGCCGTTACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGC
CAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCTGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCT
GGCTGGCGCTGGAGCGTACTCGTATTGTCAGTCCGATGACCGGTTATGTCTCCCGCCGCGCGGTACAGCCTGGGGCGCAA
ATTAGCCCAACGACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACTTTAAAGAGACGCAGAT
TGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACCGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGG
TTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCGGCGCAAAATGCGACCGGTAACTGGATCAAAGTCGTT
CAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATACCCGCTGCGTATCGGTTTATCCACGCTGGT
GAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATAAAGTACGTTCCACTCCGGTAGCGGTAAGCACCGCGC
GTGAAATCAGCCTGGCACCTGTCAATAAACTGATCGACGATATCGTAAAAGCTAACGCTGGCTAA

Upstream 100 bases:

>100_bases
CAAAAATCCAGATTTATAAAAGAAAAAATGACCGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCA
GCCGATGAGTTAAGAAGATC

Downstream 100 bases:

>100_bases
TCCAGAGGTGCGTGTGATGCAACAGCAAAAACCGCTGGAAGGCGCGCAACTGGTCATTATGACGATTGCGCTGTCACTAG
CGACATTCATGCAGGTGCTG

Product: multidrug resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MENNMSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVW
ADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIG
REELQHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQ
ISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVV
QRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG

Sequences:

>Translated_394_residues
MENNMSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVW
ADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIG
REELQHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQ
ISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVV
QRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG
>Mature_394_residues
MENNMSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVW
ADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIG
REELQHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQ
ISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVV
QRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=390, Percent_Identity=99.4871794871795, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1788711, Length=378, Percent_Identity=49.2063492063492, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1790519, Length=310, Percent_Identity=30, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081951, Length=314, Percent_Identity=27.3885350318471, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789637, Length=317, Percent_Identity=25.2365930599369, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43226; Mature: 43226

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNMSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAY
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTH
EECCEEEEEEHHCCCEEEEECCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREELQHARDAVTSAQAQLDV
HHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQ
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
ISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGT
CCCCCCEEEEECCCCEEEECCCCCCEEECEECCCCEEEEEECCCCCCEECCEEEEEECCC
GSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLA
CCCEEEECCCCCCCHHHHHHHHCCCEEEECHHHHHHCCHHCCHHHEEEEECCCCCHHHHH
NKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG
HHHHCCCEEEEHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENNMSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAY
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTH
EECCEEEEEEHHCCCEEEEECCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREELQHARDAVTSAQAQLDV
HHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQ
HHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
ISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGT
CCCCCCEEEEECCCCEEEECCCCCCEEECEECCCCEEEEEECCCCCCEECCEEEEEECCC
GSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLA
CCCEEEECCCCCCCHHHHHHHHCCCEEEECHHHHHHCCHHCCHHHEEEEECCCCCHHHHH
NKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG
HHHHCCCEEEEHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]