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The map label for this gene is proC [H]

Identifier: 209396678

GI number: 209396678

Start: 468199

End: 469008

Strand: Reverse

Name: proC [H]

Synonym: ECH74115_0460

Alternate gene names: 209396678

Gene position: 469008-468199 (Counterclockwise)

Preceding gene: 209399948

Following gene: 209399711

Centisome position: 8.42

GC content: 54.81

Gene sequence:

>810_bases
ATGGAAAAGAAAATCGGTTTTATTGGCTGCGGCAATATGGGAAAAGCCATTCTCGGCGGTCTGATTGCCAGCGGTCAGGT
GCTTCCAGGGCAAATCTGGGTATATACCCCCTCCCCGGATAAAGTCGCCGCCCTGCATGACAAGTTCGGCATCAACGCCG
CAGAATCGGCGCAAGAAGTGGCGCAAATCGCCGACATCATTTTTGCCGCCGTTAAACCTGGCATCATGATTAAAGTGCTT
AGCGAAATCACCTCCAGCCTCAATAAAGACTCTCTGGTCGTTTCTATTGCTGCAGGTGTCACGCTCGACCAGCTTGCCCG
CGCGCTGGGCCATGACCGGAAAATTATCCGCGCCATGCCGAACACTCCCGCGCTGGTTAATGCCGGGATGACCTCCGTAA
CGCCAAACGCGCTGGTAACCCCAGAAGATACCGCTGATGTGCTGAATATTTTCCGCTGCTTTGGCGAAGCGGAAGTAATT
GCTGAGCCGATGATCCACCCGGTGGTCGGTGTGAGCGGTTCTTCGCCAGCCTACGTCTTTATGTTTATCGAAGCGATGGC
CGACGCCGCCGTGCTGGGCGGGATGCCACGCGCCCAGGCGTATAAATTTGCCGCTCAGGCGGTAATGGGTTCCGCAAAAA
TGGTGCTGGAAACGGGAGAACATCCGGGGGCACTGAAAGATATGGTCTGCTCACCGGGAGGCACCACCATTGAAGCGGTA
CGCGTACTGGAAGAGAAAGGCTTCCGTGCCGCAGTGATCGAAGCGATGACGAAGTGTATGGAAAAATCAGAAAAACTCAG
CAAATCCTGA

Upstream 100 bases:

>100_bases
TAATCCTCTATTGTGTCGCGCTTTTGCCTTCCGGCATAGTTCTGTTTATGCTTCTGCCAGCGATTATCAAAACAATGAAT
TTCACGGCAGGAGTGAGGCA

Downstream 100 bases:

>100_bases
TGACTTTCGCCGGACGTCAGGCCGCCACTTCGGTGCGGTTACGTCCGGCTTTCTTTGCTTTGTAGAGCGCCAAATCTGCC
GATTTCAACCACTCGCGATA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Sequences:

>Translated_269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
>Mature_269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI24797097, Length=266, Percent_Identity=38.3458646616541, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI24797095, Length=266, Percent_Identity=38.3458646616541, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI21361454, Length=266, Percent_Identity=38.3458646616541, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI198041662, Length=266, Percent_Identity=34.5864661654135, Blast_Score=168, Evalue=5e-42,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=99.6282527881041, Blast_Score=544, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17569021, Length=262, Percent_Identity=40.0763358778626, Blast_Score=184, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17540664, Length=297, Percent_Identity=35.3535353535354, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320861, Length=272, Percent_Identity=29.0441176470588, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648116, Length=277, Percent_Identity=38.2671480144404, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=38.8679245283019, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=41.4201183431953, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 28145; Mature: 28145

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDKFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDKFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6296787; 9278503 [H]