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The map label for this gene is yneF [H]

Identifier: 209396620

GI number: 209396620

Start: 2049420

End: 2050838

Strand: Reverse

Name: yneF [H]

Synonym: ECH74115_2135

Alternate gene names: 209396620

Gene position: 2050838-2049420 (Counterclockwise)

Preceding gene: 209395871

Following gene: 209396611

Centisome position: 36.81

GC content: 48.84

Gene sequence:

>1419_bases
ATGCATGTACACCCCATTTCAACTTTCCGTGTGTTCCAGGAAGGTCATCTGCTACGTAATAGCATCGCTATTTTTGCGCT
AACCACGCTGTTCTATTTTATTGGTGCAGAGTTACGGCTGGTTCACGAACTTTCTCTTTTCTGGCCGCTGAATGGCGTAA
TGGCGGGGGTGTTTGCCCGCTATGTCTGGCTTAATCGACTGCATTACTATGCGATCAGTTATGTGGCGATGCTGGTTTAT
GATGCCATAACCACCGAATGGGGACTGGTTTCACTGGCTATCAATTTCTCCAATATGATGTTTATTGTTACCGTCGCCTT
ACTGGTCGTGCGGGATAAGCGTTTGGGGAAAAATAAATATGAGCCAGTAAGTGCCTTACGGCTATTTAATTACTGTCTGA
TTGCCGCATTATTATGCGCTATTGTCGGGGCGATTGGTTCGGTCAGTATTGATAGTCTGGATTTCTGGCCTTTGCTTGCG
GACTGGTTCAGTGAGCAATTCTCAACGGGCGTGTTGATCGTGCCTTGTATGCTGACGTTGGCTATTCCTGGAGTGCTGCC
GCGCTTTAAAGCAGAACAGATGATGCCCGCCATCGCGCTTATTGTGTCGGTTATTGCCTCGGTAGTCATTGGCGGAGCGG
GGAGTCTGGCGTTCCCGCTGCCTGCATTAATCTGGTGTGCAGTGCGCTATACGCCGCAGGTAACATGTCTGTTGACCTTT
GTCACCGGTGCGGTGGAAATCGTACTGGTGGCTAATTCGGTGATTGATATCTCGGTCGGTTCGCCGTTCTCCATTCCGCA
AATGTTCTCCGCACGTCTCGGTATTGCCACGATGGCGATATGCCCAATTATGGTTTCTTTTAGCGTGGCAGCGATCAATT
CGCTAATGAAGCAAGTTGCGCTGCGAGCCGACTTTGATTTTCTGACTCAGGTTTACTCACGGTCCGGTCTTTATGAGGCG
CTGAAAAGTCCATCGCTGAAACAGACACAACATCTGACTGTCATGCTACTTGATATCGACTATTTCAAAAGTATTAACGA
TAACTATGGACATGAATGTGGCGATAAAGTGTTAAGCGTGTTTGCCCGGCATATTCAGAAGATTGTCGGTGATAAGGGGC
TGGTGGCGCGCATGGGCGGCGAAGAATTTGCTGTTGCAGTGCCGTCAGTGAATCCTGTCGATGGTCTGCTAATGGCGGAA
AAAATCCGTAAAGGCGTTGAACTGCAACCATTCACCTGGCAACAAAAAACGCTTTATCTCACGGTAAGTATTGGCGTCGG
TAGTGGTCGCGCATCGTACCGAACGCTGACCGATGACTTTAATAAATTGATGGTCGAAGCCGATACCTGTCTGTATCGCT
CGAAGAAAGATGGGCGCAACCGTACCAGCACTATGCGTTACGGTGAGGAAGTTGTCTGA

Upstream 100 bases:

>100_bases
CTTTATTTGCCGCCGCTGTCAGCGAACATGCTATCCTTTATGAGAATTTACTTATTACGTTGAGATAATTGCCACCCGCA
ATTGTATGGACAGATAATTA

Downstream 100 bases:

>100_bases
CATCTTTATTCATTCCGCGCGATTTGAATGCAATGTGTTCTACCACTTTATGATCCAGGAAAAGGCGTATTTCCTCGATT
AAACCATGATCCGCGCCACA

Product: diguanylate cyclase (GGDEF) domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVY
DAITTEWGLVSLAINFSNMMFIVTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
VTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEA
LKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAE
KIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Sequences:

>Translated_472_residues
MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVY
DAITTEWGLVSLAINFSNMMFIVTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
VTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEA
LKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAE
KIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
>Mature_472_residues
MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVY
DAITTEWGLVSLAINFSNMMFIVTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
VTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEA
LKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAE
KIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=315, Percent_Identity=99.3650793650794, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=37.4269005847953, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=212, Percent_Identity=30.188679245283, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=36.2068965517241, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786584, Length=201, Percent_Identity=28.3582089552239, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=193, Percent_Identity=28.4974093264249, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=30.939226519337, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=30.939226519337, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI145693134, Length=133, Percent_Identity=32.3308270676692, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=33.1460674157303, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 52167; Mature: 52167

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure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EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]